Study on microRNA-9-5p reducing sensitivity of breast cancer cells to doxorubicin through targeting HIC1

doi:10.16139/j.1007-9610.2020.03.011

Abstract

Abstract:

Objective To study the effect of microRNA-9-5p (miR-9-5p) which regulate hypermethylated in cancer 1 (HIC1) in reducing sensitivity of breast cancer MDA-MB-231 cells to doxorubicin (DOX) and the mechanisms. Methods ①Up-regulation or down-regulation of miR-9-5p in cells was constructed by lentivirus transfection. Cell viability and apoptosis were detected with CCK-8 and flow cytometry after adding different concentrations of DOC. ②Expression of HIC1 was detected by reverse transcription-polymerase chain reaction and Western blotting when miRNA-9-5p was overexpressed or inhibited. The interaction between miR-9-5p and HIC1 was analyzed by database and luciferase assay. ③HIC1 was down-regulated to detect cell viability and apoptosis after adding different concentrations of DOX. Results ①Up-regulation of miR-9-5p increased cell viability, and reduced apoptosis. Down-regulation of miR-9-5p reduced cell viability and increased apoptosis. ②miR-9-5p directly targeted HIC1 and negative relationship of expression was present between miR-9-5p and HIC1. ③Down-regulation of HIC1 could reverse the down-regulation effect of miR-9-5p. Conclusions miR-9-5p reduces the sensitivity of breast cancer cells to DOX through down-regulation of HIC1.

Key words: Breast cancer, MicroRNA-9-5p, Hypermethylated in cancer 1, Doxorubicin, Sensitivity

CLC Number:

R737.9

GAO Hang, ZHAO Feng, WU Yan, PEI Wenjiang, ZHONG Ming, GU Yan, GUO Shanyu, DAI Qiancheng, ZHANG Wei. Study on microRNA-9-5p reducing sensitivity of breast cancer cells to doxorubicin through targeting HIC1[J]. Journal of Surgery Concepts & Practice, 2020, 25(03): 227-233.

Figures/Tables 9

References 25

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