Abstract:

Objective To explore the impact of miR-4674 expression changes on the biological characteristics of BGC-823 gastric cancer cell line based on bioinformatics research. Methods Through bioinformatics screening, miR-4674 was prioritized as a gastric cancer-associated miRNA. We constructed miR-4674 mimic, inhibitor, and corresponding negative control (NC) transfected into the BGC-823 cell line. Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression changes of miR-4674 in BGC-823 cells. Functional assays included: MTT assay for cell proliferation ability; Transwell assay for migration capacity; TUNEL staining for cell apoptosis detection.Results Compared with the control group and cells transfected with NC, the level of miR-4674 was significantly increased in cells transfected with miR-4674 mimic, and the proliferation and migration abilities of the cells were significantly improved (P< 0.05). In cells transfected with miR-4674 inhibitor, the level of miR-4674 was significantly decreased, and the proliferation and migration abilities of the cells were significantly reduced (P<0.05). The result of TUNEL showed no significant differences in apoptotic rates were observed across all groups. Conclusions In the BGC-823 gastric cancer cell line, inhibition of miR-4674 expression can reduce its malignancy, while overexpression of miR-4674 can enhance its malignancy, suggesting its potential as a therapeutic target for gastric cancer intervention, providing a new strategy for the treatment of gastric cancer.

Key words: miR-4674, Gastric cancer, BGC-823 cell line, Malignancy degree