组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2016, Vol. 12 ›› Issue (1): 6-10.doi: 10.3969/j.issn.1673-0364.2016.01.002

• 论著 • 上一篇    下一篇

SDF-1-CXCR4/CXCR7信号对脂肪干细胞促血管新生能力的影响

李强，张林霞，张爱君，陶常波，李雪阳，马志兵，金培生   

  1. 徐州医学院附属医院整形外科；中国医学科学院整形外科医院
  • 发布日期:2020-07-23

The Influence of Stromal Cell-derived Factor-1 Promoting Angiogenesis of Adipose-derived Stem Cells via CXCR4 and CXCR7 Pathway in vitro

LI Qiang,ZHANG Linxia,ZHANG Aijun,TAO Changbo,LI Xueyang,MA Zhibing,JIN Peisheng   

  1. 1. Plastic Surgery Department, Xuzhou Medical College Affiliated Hospital, Xuzhou 221000, China; 2 Plastic Surgery Hospital, Peking Union Medical College ＆ Chinese Academy of Medical Sciences, Beijing 100144, China）
  • Published:2020-07-23

摘要： 目的 研究SDF-1对ADSCs体外促血管新生能力的影响,并探讨CXCR4、CXCR7在其中的作用。方法 体外培养ADSCs,检测其CXCR4、CXCR7的表达;SDF-1刺激ADSCs,并分别加入CXCR4、CXCR7封闭抗体,检测ADSCs-CM中VEGF、HGF、β-FGF含量,及其对h UVECs微血管形成的影响。结果 成功培养ADSCs;ADSCs体外培养传代过程中CXCR4、CXCR7表达持续下降;SDF-1刺激上调CXCR4、CXCR7表达,并提高ADSCs对血管活性因子的分泌,及促进h UVEC微血管的形成;封闭CXCR4、CXCR7均可显著抑制SDF-1的促进作用。结论 体外环境下,SDF-1可上调ADSCs中CXCR4、CXCR7表达,并通过SDF-1-CXCR4/CXCR7两条通路提高ADSCs的促血管新生能力。

关键词: 脂肪干细胞, 基质细胞衍生因子-1, 血管新生

Abstract: Objective To investigate the role of SDF-1 in promoting ADSCs angiogenesis, as well as CXCR4, CXCR7.Methods ADSCs were primary cultured by enzyme digestion method. Flow cytomertry and western blot assays were performed to detect the expression of CXCR4, CXCR7. ADSCs were treated with SDF-1 and neutralizing anti-CXCR4 and/or anti-CXCR7 antibody. ELISA was used to detect the secretion of VEGF, HGF, and β-FGF. h UVECs tube formation assay was used to examine the effect of SDF-1 and neutralizing antibody CXCR4, CXCR7 on promoting angiogenesis property of ADSCs. Results ADSCs were successfully isolated and cultured from human liposuction tissue. CXCR4 and CXCR7 expression were significantly decreased during cell culture. When treated with SDF-1, CXCR4 and CXCR7 expression were significantly increased. In addition, SDF-1 pretreating also promoted the secretion of angiogenesis factors VEGF, HGF, β-FGF and increased the ability of tube formation of h UVECs induced by ADSCs. The blocking of CXCR4 and CXCR7 activation also inhibited the promotion of angiogenesis property of ADSCs caused by SDF-1. Conclusion SDF-1 pretreating can up-regulate the expression of CXCR4 and CXCR7 in ADSCs. Furthermore, it also promotes the angiogenesis ability of ADSCs through CXCR4 and CXCR7 pathways.

Key words: Adipose-derived stem cells, Stromal cell-derived factor-1, Angiogenesis

中图分类号: