Effects of FAK SiRNA On the Expression of Integrinα1,TGF-βR,FAK and α-SMA in Hypertrophic Scar Fibroblasts In Vitro

doi:10.3969/j.issn.1673-0364.2008.06.006

Journal of Tissue Engineering and Reconstructive Surgery ›› 2008, Vol. 4 ›› Issue (6): 320-322.doi: 10.3969/j.issn.1673-0364.2008.06.006

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Effects of FAK SiRNA On the Expression of Integrinα1,TGF-βR,FAK and α-SMA in Hypertrophic Scar Fibroblasts In Vitro

CHEN Rui¹,FAN Zhihong¹,FU Mingang¹,WANG Ling¹,PING Ping²

1 Department of Plastic Surgery;Renji Hospital;Shanghai Jiaotong University School of Medicine;2 Department of Urology Surgery;Shanghai 200127;China.;

Published:2020-07-23
Contact: 上海市卫生局面上项目(054042);;上海市教委科研创新项目(08YZ40);;上海交通大学医学院附属仁济医院临床基础合作项目(074119606)

Abstract

Abstract: Objective Explore the effects of FAK SiRNA on the expression of integrin α1, TGF-βR, focal adhesive kinase (FAK) and α-smooth muscle actin (α-SMA) of hypertrophic scar fibroblasts. Methods After hypertrophic scar fibroblasts were transfected by FAK SiRNA, FQ-PCR assay was applied to detect the mRNA expression of integrin α1, TGF-βR, FAK, α-SMA. Results 48 hours after transfection the mRNA expression of integrin α1, TGF-βR, FAK, α-SMA was significantly lower than before transfection (P<0.05). Conclusion F...

Key words: Hypertrophic scar, Focal adhesive kinase, Small interfering RNAs （SiRNA）, FQ-PCR

CLC Number:

R318.04

CHEN Rui,FAN Zhihong,FU Mingang,WANG Ling,PING Ping. Effects of FAK SiRNA On the Expression of Integrinα1,TGF-βR,FAK and α-SMA in Hypertrophic Scar Fibroblasts In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2008, 4(6): 320-322.

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Effects of FAK SiRNA On the Expression of Integrinα1,TGF-βR,FAK and α-SMA in Hypertrophic Scar Fibroblasts In Vitro

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