Abstract: Objective To investigate the feasibility of allogeneic ADSCs to repair the tubular bone defects. Methods Scapular fat was isolated from SD Rats, and further cultured in vitro (rADSCs). rASCs of P3 were seeded onto demineralized bone matrix (DBM) and cultured in osteogenic medium after 24 hours. The adhesion and proliferation of rA DSCs after seeded onto DBM were observed and the osteogenic potential of rASCs was tested. Bilateral tubular bone defects of rats were created. The defects were repaired with rADSCs-DBM constructs (experimental group) or DBM alone (control group). After 8 and 24 weeks, DR testing and histological examination were performed to observe the bone regeneration. Results rADSCs adhere to the surface of DBM well and grow fine in vitro. The osteogenic potential of rASCs was maintained. 24 weeks after implantation, DR testing showed that new bones were formed in experimental group, while no bone tissue formation in control group. Histological examination revealed that the defect was repaired by typical bone tissue in experimental group, while only minimal bone formation with fibrous connection was observed in control group. Conclusion The allogeneic rADSCs with DBM scaffolds can repair critical-sized bone defects successfully.

Key words: Bone defect, Bone tissue engineering, Adipose derived stem cells, Demineralized bone matrix