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Table of Content

    23 July 2020, Volume 9 Issue 5 Previous Issue    Next Issue
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    Original article
    Expression of ADAM10 During Craniomaxillofacial Intramembranous Ossification of Mice
    TAN Yu,FU Runqing,FANG Bing,YANG Zhi
    2013, 9 (5):  241-244.  DOI: 10.3969/j.issn.1673-0364.2013.05.001
    Abstract ( 400 )  
    Objective To investigate the expression of ADAM10 during craniomaxillofacial ossification of mice. Methods Alcian blue and Alizarin red staining were used to detect the intramembranous ossification areas during craniomaxillofacial development of wild-type C57BL/6 mice. Expression of ADAM10 in mice craniomaxillofacial bone was analyzed by immunohistochemistry. The subcellular localization of ADAM10 in MC3T3 was observed by double immunofluorescent staining and the expression of ADAM10 in mice mandibular was determined by western blot. Results Skull, nasal cartilage, maxilla, palate plate and mandibular of mice displayed intramembranous ossification and expression of ADAM10 showed strong singal in these regions, especially the regions undergoing intramembranous ossification. In MC3T3, ADAM10 distributed in cytoplasm, especially in cell membrance and cell nucleus, and the high expression signal of ADAM10 near cell nucleus was co-localized with GM130, a marker of Golgi, indicating ADAM10 decorated in Golgi before transported to the cell membrance. The expression of ADAM10 in mice mandibular was significantly down-regulated when grown up. Conclusion ADAM10 is widely expressed in mice intramembranous ossification regions, providing the evidence of its regulation in intramembranous ossification of mice.
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    Allogeneic Adipose Derived Stem Cells Combining with Demineralized Bone Matrix for Repairing of Rat Ulnar ;Defects
    XIONG Zhuyou,FANG Xiaokui,XU Jing,ZHANG Li,WANG Chen,LI Guangzao
    2013, 9 (5):  245-270.  DOI: 10.3969/j.issn.1673-0364.2013.05.002
    Abstract ( 323 )  
    Objective To investigate the feasibility of allogeneic ADSCs to repair the tubular bone defects. Methods Scapular fat was isolated from SD Rats, and further cultured in vitro (rADSCs). rASCs of P3 were seeded onto demineralized bone matrix (DBM) and cultured in osteogenic medium after 24 hours. The adhesion and proliferation of rA DSCs after seeded onto DBM were observed and the osteogenic potential of rASCs was tested. Bilateral tubular bone defects of rats were created. The defects were repaired with rADSCs-DBM constructs (experimental group) or DBM alone (control group). After 8 and 24 weeks, DR testing and histological examination were performed to observe the bone regeneration. Results rADSCs adhere to the surface of DBM well and grow fine in vitro. The osteogenic potential of rASCs was maintained. 24 weeks after implantation, DR testing showed that new bones were formed in experimental group, while no bone tissue formation in control group. Histological examination revealed that the defect was repaired by typical bone tissue in experimental group, while only minimal bone formation with fibrous connection was observed in control group. Conclusion The allogeneic rADSCs with DBM scaffolds can repair critical-sized bone defects successfully.
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    Construction of Tissue Engineered Meniscus with Bone Marrow Mesenchymal Stem Cells in Vitro
    LI Dan,ZHOU Guangdong,LIU Yi,YIN Zongqi,WANG Zhenxing,ZHU Lian
    2013, 9 (5):  251-278.  DOI: 10.3969/j.issn.1673-0364.2013.05.003
    Abstract ( 349 )  
    Objective To explore the techniques of constructing tissue engineered meniscus with bone marrow mesenchymal stem cells (BMSCs) in dog model in vitro. Methods Biodegradable scaffolds in shape of meniscus were fabricated with polyglycolic acid (PGA) and polylactic acid (PLA) by mold. BMSCs were harvested from bone marrow of dogs and cultured in vitro. Then the expanded cells were seeded onto the scaffolds, and transferred to chondrogenic medium after 5 days. Gross observation, histological observation and biomechanical test were carried out after cultured for 6 weeks. Results The constructs cultured in vitro for 6 weeks maintained three-dimensional structure of meniscus approximately, and formed smooth white cartilage-like tissue with elasticity. HE staining showed typical mature lacuna, suggesting the formation of mature cartilage tissue. Safranin'O staining showed abundant proteoglycan. Biomechanical analyses indicated that Young's modulus of constructs can reach 13.2% of native meniscus. Conclusion BMSCs can differentiate into heterogeneous cartilage-like tissue successfully through cultivation of inducing system, and construct tissue engineered meniscus in vitro.
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    Biological Characteristics of Bone Marrow Mesenchymal Stem Cells Expressing hTERT Gene
    MA Di,YAN Jiqi,PENG Chenghong,SHI Minmin,CHEN Xuehua,WANG Weijie,LI Hongwei
    2013, 9 (5):  256-259.  DOI: 10.3969/j.issn.1673-0364.2013.05.004
    Abstract ( 371 )  
    Objective To identify the biological characteristics after infecting the rat BMSCs with recombinant virus supernatant containing hTERT gene. Methods BMSCs were infected with hTERT recombinant virus supernatant (MSC-hTERT group) and empty virus supernatant (MSC-GFP group), the uninfected group (MSC group) was also set up. The expression of target gene was detected by realtime PCR. Number of passages, cell morphology and cell cycle in MSC group and MSC-hTERT group were observed and analyzed. Cell markers of cells in MSC-hTERT group were detected and the potential of differentiation was also evaluated. Results The expression of hTERT was higher in MSC-hTERT group than in the other two groups. Number of passages and proliferation index in MSC-hTERT group were higher than in MSC group. The ability of multi-lineage differentiation potential of cells in MSC-hTERT group was maintained. Conclusion MSCs transfected by hTERT have the same biological characteristics as primary BMSCs and the ability of cell proliferation was strengthened. More cells could be reserved for tissue engineering and cell transplantation therapy.
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    The Effects of Sertoli Cells on the Induced Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
    YING Hanru,JIANG Lihua,WANG Weilun,LI Weiyi
    2013, 9 (5):  260-262.  DOI: 10.3969/j.issn.1673-0364.2013.05.005
    Abstract ( 325 )  
    Objective To explore the effects of Sertoli cells on the induced adipogenic, osteogenic and neuroblastic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were isolated and purified with the density gradient centrifugation method, Sertoli cells were isolated with two-step enzyme digestion;The adipogenic, osteogenic and neuroblastic differentiation of P3/P4 BMSCs were induced. Sertoli cells were co-cultured with BMSCs and then the morphological change of BMSCs were observed. Results After adipogenic induction, the lipid droplets that have strong refraction in the cytoplasm were observed by oil red O staining. The intracellular lipid droplets shrunk and the orange stained lipid droplets were hard to observe after adding Sertoli cells; After osteogenic induction, calcium nodules were shown by alizarin red S staining. More clearer calcium nodules were observed and the red-staining was stronger after adding Sertoli cells. No morphological change was observed on BMSCs before and after adding Sertoli cells. Conclusion Sertoli cells could promote BMSCs’ osteogenic differentiation, inhibit their adipogenic differentiation and have no effect on their neuroblastic differentiation.
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    The Influence of Scar Tissue on Surgical Site Infection and Bacterial Colonization
    LIU Wenhui,HUANG Xiaolu,XIE Yun,LI Qingfeng
    2013, 9 (5):  263-267.  DOI: 10.3969/j.issn.1673-0364.2013.05.006
    Abstract ( 378 )  
    Objective To compare the surgical site infection rate of scar tissue and normal tissue; To determine the difference of leading pathogens colonization and their resistance to common antibiotics between scar tissue and normal tissue. Methods A prospective cohort study was conducted among 129 patients who received plastic and reconstructive surgery and were divided into scar group or non-scar group according to whether scars were involved in their surgery or not. Data on background information, risk factor for bacterial colonization and related confounding factors were collected. The diagnose criteria of SSI was in consistence with the guideline of the Ministry of Health. Microorganism specimen was taken before the surgery. Further identification was undertaken to determine the colonization pattern by antibiotics sensitivity analysis. Multiple Logistic regression analysis was conducted to investigate the association between risk factors and bacterial colonization profile. Results Scar tissue was featured with more surgical site infection (2.3 times, 4.8% vs. 2.1%, P<0.05) and bacterial colonization (1.4 times, 73.2%vs. 53.2%, P<0.05) than normal tissue. Bacterial colonization of scar tissue was significantly connected with factors such as electricity, trauma, chemical injury, site of head and neck. Staphylococcus aureus, as the most common leading pathogen, accounted for 22.4% of all the pathogens and 19.4% of it was found multiple antibiotic resistant. Conclusion Scar tissue is an independent risk factor for surgical site infection with more antibiotic resistant bacteria than normal tissue. Bacterial colonization was significantly related to several etiological factors and anatomic site.
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    Clinical Observation of Multi-modalities Therapy in Auricular Keloid
    ZHANG Wei,ZHANG Huifeng,ZHENG Jianyun,LEI Huibin,WANG Fang,CHEN Ying
    2013, 9 (5):  268-270.  DOI: 10.3969/j.issn.1673-0364.2013.05.007
    Abstract ( 375 )  
    Objective To evaluate the clinical effects of surgery combining with early local injection of triamcinolone acetonide in the treatment of auricle keloid. Methods Twelve patients (20 auricles) with keloid were treated by surgery. The keloids were partly or completely excised and corrected with local skin flap. Triamcinolone acetonide 10~20 mg diluted with 2% lidocaine was injected into the incision or scar tissue once 2 weeks 10 days after operation. Administration of this drug was gradually tapered according to the reduction of the scar. Results All the patients were followed up for 6 months to 3 years. Eight cases were cured without relapse, 4 cases showed partial remission. The rate of therapeutic effect was 100%. Conclusion Surgery combining with early injection of triamcinolone acetonide was an improved therapeutic method in the treatment of auricle keloid.
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    Evaluation of the Masticatory Efficiency after Mandibular Angle Ostectomy
    BIAN Jie,GUO Jun,MENG Zhibing,CHANG Caiwang,YANG Le,HUANG Jinhua
    2013, 9 (5):  271-278.  DOI: 10.3969/j.issn.1673-0364.2013.05.008
    Abstract ( 337 )  
    Objective To evaluate the masticatory efficiency after mandibular angle ostectomy. Methods Fifteen patients who accepted mandibular angle ostectomy (bilateral jaw edge of the mandibular angle resection and mandibular body outer panel split) were selected. Periodontal disease, temporomandibular joint pain and partial jaw were screened. The masticatory efficiency of the patients were evaluated by means of sieving and weighing almond and jujube before operation, 1 month and 6 months after operation. Results There were significant differences between the average masticatory efficiency 1 month after operation and before operation (P0.05). Conclusion Mandibular angle ostectomy has no effect on the masticatory efficiency.
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    The Efficacy and Safety of Locking Plate Internal Fixation for the Treatment of Complex Tibial Plateau Fractures
    WANG Fang,SHI Fajian,ZHAO Xiaolong
    2013, 9 (5):  273-275.  DOI: 10.3969/j.issn.1673-0364.2013.05.009
    Abstract ( 358 )  
    Objective To investigate the clinical efficacy and safety of locking plate internal fixation for the treatment of complex tibial plateau fractures. Methods From April 2010 to December 2012, 110 patients with complex tibial plateau fracture were divided into observation group (n=55) and control group (n=55). Patients in observation group were treated with locking plate internal fixation and patients in control group were treated with anatomic plate internal fixation. The general information, complications and clinical outcome of two groups were observed and compared. Results There were no significant differences in operation time and anatomical restoration rate between two groups (P>0.05). However, the peri-operative bleeding, time of hospitalization, loading time after operation, fracture healing time, range of knee motion 6 months after operation, HSS score of knee joint function 6 months after operation, complication rate and efficacy in observation group were significantly better than those in control group (P<0.05). Conclusion Locking plate internal fixation is effective and reliable for the treatment of complex tibial plateau fractures and is worthy of popularization.
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    Clinical Effects of Expert Tibial Nail in Treating Distal Tibial Fracture
    ZHU Weiguo
    2013, 9 (5):  276-278.  DOI: 10.3969/j.issn.1673-0364.2013.05.010
    Abstract ( 481 )  
    Objective To investigate the clinical effects of expert tibial nail in treating distal tibial fracture. Methods From January 2010 to December 2012, 74 patients with distal tibial fracture were randomly divided into 2 groups, experimental group (n=37) and control group (n=37). Patients in experimental group were treated with expert tibial nail fixation while patients in control group were treated with anatomical plate fixation. The surgical information and postoperative recovery of the two groups were compared. Results There was no significant differences of operative time, blood loss, times of X-ray exam, postoperative hospital stay and incidence of major complications between the two groups ( P>0.05);Preoperative hospital time, total hospitalization time, full weight bearing time and bone healing time of experimental group were shorter than that of control group (P0.05). Conclusion Expert tibial nail is effective in treating tibial fracture, which is conducive to the patient's early recovery, and is worthy of promotion.
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    Cannulated Screws Combining Limited incision for the Fixation of Garden III, IV Type Femoral Neck Fractures in Younger Adults
    LI Jiade,CHEN Yu,ZHONG Yongxiang,CHEN Yongzhi,Xin Weiguang,HUANG Yang
    2013, 9 (5):  279-281.  DOI: 10.3969/j.issn.1673-0364.2013.05.011
    Abstract ( 410 )  
    Objective To investigate the efficacy of cannulated screw combining limited incision for the treatment of Garden Ⅲ, Ⅳ type femoral neck fractures in younger adults. Methods From September 2009 to July 2011, 26 cases of femoral neck fractures were treated using cannulated screw combining limited incision. The clinical efficacy was evaluated according to Harris score. Results All the patients were followed up for 6 months to 3 years (mean 1.6 years). The incisions were healed by first intension and the fractures were healed in 7 months on average. According to Harris score, 13 cases were evaluated as excellent, 8 cases as good, 4 cases as fair and 1 case as poor, the choice rate was 81%. Conclusion Cannulated screw combining limited incision is effective for the treatment of Garden III, IV type femoral neck fractures in younger adults.
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    The Efficacy of Thromboprophylaxis after Surgical Treatment of Ankle Fractures
    WANG Jianjun,XUE Shuyu,XING Caiyun,LI Dapeng,YOU Youfeng
    2013, 9 (5):  282-284.  DOI: 10.3969/j.issn.1673-0364.2013.05.012
    Abstract ( 302 )  
    Objective To explore the effects of risk factors and preventive treatments of thrombus on the incidence of thromboembolic events. Methods From January 2008 to March 2013, 328 patients underwent surgical treatment ankle fracture were conducted in this retrospective study. Charts were reviewed to identify risk factors, such as tumor, hormone use, pregnancy, blood dyscrasia, history of thromboembolic events, smoking history, obesity, dyslipidosis, atherosclerotic vascular disease, paralysis, etc. Fifty-three patients (16.15%) were treated with thromboprophylaxis (low-molecular-weight heparin or warfarin). The effects of risk factors and preventive treatments of thrombus on the incidence of thromboembolic events were statistical analysed. Results The incidence of thromboembolic events was 2.74% (9 cases), with 2.13% (7 cases) involving deep venous thrombosis and 0.61% (2 cases) involving nonfatal pulmonary embolism. One hundred and forty eight patients (45.1%) had one or more risk factors. Patients with one or more risk factors had a greater risk of thromboembolic events (relative risk 0.66). No significant correlation could be identified between the occurrence of thromboembolic events and risk factors or preventive treatments of thrombus. Conclusion Thromboprophylaxis should not be suggested after the surgical treatment of ankle fractures.
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    Research Progress of Scaffold for Bladder Tissue Engineering
    ZHAO Yang,ZHANG Ming,WANG Zhong,LU Mujun
    2013, 9 (5):  289-291.  DOI: 10.3969/j.issn.1673-0364.2013.05.014
    Abstract ( 367 )  
    The bladder scaffold for tissue engineering is vital to bladder repair. In recent years, there were an increasing number of experiments and clinical studies on bladder repair and reconstruction by using tissue engineering technology. It is necessary to pay more attention to the research of scaffold for bladder tissue engineering. In this paper, the research progress of tissue engineering scaffold for bladder repair was reviewed.
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    Research Progress in Mechanisms of Macrophages Participating in Skeletal Muscle Regeneration
    QIAO Juan,HAO Xiaoyan,GUO Shuzhong
    2013, 9 (5):  292-294.  DOI: 10.3969/j.issn.1673-0364.2013.05.015
    Abstract ( 537 )  
    In recent years, many researches have demonstrated that macrophages play a vital role in organ or tissue regeneration. Macrophages are necessary for skeletal muscle regeneration after injury. The recruitment of circulating mono-cytes/macrophages was mediated through myogenic cells and muscle resident macrophages. Macrophages are involved in both phases of skeletal muscle regeneration: first, swallowing necrotic myo-fibers, then the phenotype of macrophages switched from pro-inflammatory to anti-inflammatory. Second, factors secreted by anti-inflammatory macrophages improved the differentiation of myogenic cells and resulted in regeneration of skeletal muscle.
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    Research Progress of Bone Marrow Mesenchymal Stem Cells in Degenerated Intervertebral Disc
    YUAN Chaoqun,ZHANG Hailong
    2013, 9 (5):  295-297.  DOI: 10.3969/j.issn.1673-0364.2013.05.016
    Abstract ( 371 )  
    The further understanding of cellular scaffolds, cell factors, intervertebral disc (IVD) cell pathophysiology and BMSCs biology have laid the foundation of cell-based therapies for IVD degeneration. Moreover, a series of animal studies and vitro experiments also confirm the feasibility and effectiveness of cell-based therapies for IVD degeneration. It shows a wide prospect of clinical application in the future. The current research status of BMSCs in IVD tissue engineering was reviewed.
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