Journal of Tissue Engineering and Reconstructive Surgery ›› 2024, Vol. 20 ›› Issue (3): 285-.

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Enrichment of signaling pathways and dynamic expression of the FZD family during endogenous periosteum-guided bone regeneration

  

  • Online:2024-06-01 Published:2024-07-05

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Abstract:

Objective To investigate the signaling pathway enrichment and dynamic expression of FZD family during
endogenous periosteum-guided bone regeneration. Methods A porcine closed rib periosteum-guided bone regeneration
model was established， and periosteum and regenerated bone tissues were collected at seven time points for gene sequencing，
differential gene screening and enrichment analysis of their related signaling pathways. The FZD molecular family， which is
closely related to the process of bone regeneration， was selected for focused analysis， and the dynamic expression changes of
each member of FZD and the expression correlation between them were detected and analyzed. Results A total of seven
genes were persistently differentially expressed at different time points within 1 month after surgery in the experimental group
compared with the control group. Differential gene enrichment showed that there was mainly an immune response in the early
stage， and tissue development and remodeling were achieved later. The expression of each member of the FZD family
fluctuated at different time points， and the expression peak of most members was 3 days after surgery， while the fold change
of the experimental group was more obvious than that of the control group at 1 month after surgery， and FZD1 and FZD2 were
the highest， and there was a high correlation between them. Conclusion The differential genes in the process of periosteum
guided bone regeneration and the related signaling pathways screened in this study provide a theoretical basis for further
molecular mechanism studies， while the dynamic expression changes of the FZD molecular family play an important role in
the process， and the specific biological mechanisms involved in the process need to be verified by further studies.

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