Abstract: As the largest endocrine organ and the main energy storage system in the human body， adipose tissue plays a pivotal role in regulating energy homeostasis. Pathological conditions such as insulin resistance， chronic inflammation and mitochondrial dysfunction are closely related to adipose metabolic disorders. In vitro models are significant tools for studying the mechanism of associated disorders. At present， three-dimensional culture is a common model for in vitro adipose tissue research because it can imitate the process of adipose metabolism to a certain extent. In order to serve as a resource for future scientific investigations， an overview of three-dimensional adipose tissue models and their properties was provided in this paper.

Key words: Adipose tissue, 　Adipose metabolism, 　In vitro models, 　Three-dimensional culture, 　Microfluidics