Abstract: Objective To investigate the inhibitory effects of Oxymatrine coating agent on hypertrophic scar. Methods Human hypertrophic scar tissues were subcutaneously transplanted into the back of 30 nude mice to create the animal model of hypertrophic scar. Ten days after the operation, the mice were distributed into the control group, Oxymatrine coating agent group and positive medicine group, and were treated with blank coating agent, Oxymatrine coating agent and triamcinolone acetonide acetate coating agent respectively. Drugs were applied 2 times a day for 8 weeks. The growth of the scar was observed and photographed every week, and the size was measured 10 days after operation, and 35, 49, 56 days after drug treatment. HE staining was used for histological observation. RT-PCR and western blot assessment were used to detect the expression of Collagen Ⅰ, Ⅲ and α-SM-Actin. Results After drug treatment for 35, 49 and 56 days, the scar sizes in Oxymatrine coating agent group shrunk obviously and were much smaller compared with control group and positive medicine group (P<0.05). According to RT-PCR assessment, the expression of Collagen Ⅰ,Ⅲandα-SM-Actin were declined sharply in Oxymatrine coating agent group and positive medicine group, compared with the control group. Conclusion Oxymatrine coating agent has obvious inhibitory effects on hypertrophic scar, and also has anti-fibrosis effect.

Key words: Oxymatrine, Coating agent, Hypertrophic scar, Fibrosis