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23 July 2020, Volume 14 Issue 5 Previous Issue    Next Issue

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Preliminary Application of Urethral Tissue Construction with Multiple Layers Using 3D Bio -printing PI Qingmeng,WANG Xiaolei,ZHANG Zhiliang,ZHOU Biyu,REN Xiaoyun,LU Yi,HUANG Yixiong,FU Mingang,FAN Zhihong 2018, 14 (5):  241-244.  DOI: 10.3969/j.issn.1673-0364.2018.05.001 Abstract ( 349 )   Objective To investigate the feasibility of multiple-layers reconstruction of urethral tissue with 3D bioprinting technology. Methods Mixed blend of 7% GelMA and 2% Alginate with green and read microbeads respectively, or 2×106cells/mL urethral epidermal cells and smooth muscle cells, printed on the system of co-axial multiple channel bioprinting platform. The speed of inner and outer layer printing was 10-20 μL/min and CaCl2extruding was 80-200 μL/min. Then the printed structure was observed under microscopy, and the printed hollow tissues loaded urethral cells were tested with live/dead and F-actin staining from day 0 to day 7. The printed hollow structure was perfused with color medium after cultured 24 hours in vitro. Results With hydrogels mixed with microbeads, two distinct sublayers of printed hollow structure can be observed clearly under microscopy. The diameter of inner and outer layer was (663.66±51.74) μm and (976.84±63.43) μm respectively, and wall length of inner and outer layer was (62.06±10.45) μm and (93.78±10.25) μm respectively. The viability of printed urethral tissue was 84.52% and 86.26% at day 0 and day 7. F-actin expression were observed on day 7 after immunofluorescent staining and showed the spread cells. During the process of perfusion of hollow tissue, colored medium can circulate the channel smoothly without color exuding. Conclusion Urethral hollow structure can be printed with combined hydrogel by the method of co-axial multiple channel bioprinting system. And the urethral cells can survive for long term after printing. Related Articles | Metrics

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The Construction of Tissue Engineered Cartilage by Using Gelatin/Curcumin Nanofibres Membranes in Subcutaneous Model Model KANG Bokyoung,CHEN Weiming,ZHOU Guangdong,CAO Dejun 2018, 14 (5):  245-249.  DOI: 10.3969/j.issn.1673-0364.2018.05.002 Abstract ( 346 )   Objective To explore the feasibility of constructing tissue engineered cartilage by using gelatin/curcumin nanofibres membranes as scaffold in subcutaneous model. Methods The seed cells were obtained from rabbit ear cartilage. After in vitro culture and expansion, cartilage was constructed with gelatin nanofibers membrane (control group) and gelatin/curcumin nanofibers membrane (experimental group) as scaffolds. Gross observation was performed after the material was implanted into the back of the BALB/c nude mice; SEM observation of the material and cell-material complex were all performed; The cell proliferation rate of the cell-material complex was measured; The cell-material complex cultured in vitro for 1 week was implanted into the back of BALB/c nude mice. Histological examination was performed 3 and 12 weeks after implantation, and cartilage formation at different time points was compared. Results Gross observation showed that compared with the control group, the vascularization of the tissue around in the experimental group was significantly inhibited; The SEM observation showed that the chondrocyts in the two groups adhered well; Growth curve of the chondrocyts showed that curcumin had no significant effect on cell growth; Histological observation showed that the cell-material complex of both groups had cartilage lacuna formation after implanted for 3 and 12 weeks, but compared with the control group, the material in experimental group showed obvious absorption, and the cartilage matrix was thicker and more homogenization. Conclusion Gelatin/curcumin membranes could be successfully used as scaffold in the construction of tissue engineering cartilage in subcutaneous animal model. It is a kind of scaffold material with potential application prospect. Related Articles | Metrics

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Tissue Engineered Biofilm Dynamic Culture System Based on Medium Pressure Control ZHAO Yanyuan,YAN Huangping,CHEN Yanping,ZENG Shaojun,YIN Hao 2018, 14 (5):  250-257.  DOI: 10.3969/j.issn.1673-0364.2018.05.003 Abstract ( 397 )   Objective To investigate the culture system that can control the culture pressure and realize the dynamic culture of tissue engineered biofilm. Methods According to the basic principle of Pascal's law, a control method of media pressure is designed, and a tissue engineered biofilm dynamic culture system is established. The system is composed of culture dish, peristaltic pump, pressure control module, main controller and detection module. Results This system can realize the pressure control of the culture with a pressure range of 0.12-19.76 mmHg. Conclusion This system provides the pressure environment of biofilm in suitable pressure range required for culture. It provides a new method for tissue engineered biofilm dynamic culture technology. Related Articles | Metrics

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Improve ment of the Survival of Human Autologous Fat Transplantation with Chitosan Sustained Release FGF2 Gene Carrier MA Li,JIANG Sijing,JIA Daping,ZHAO Yu 2018, 14 (5):  253-257.  DOI: 10.3969/j.issn.1673-0364.2018.05.004 Abstract ( 399 )   Objective To construct a novel core-shell-structured chitosan particles with sustained-release FGF2 plasmids (the core was pFGF2-EGFP-loaded TACS and the shell was HBC), and to explore the effect of the particles on the survival rate of adipose tissue transplantation. Methods The core-structured particles (TACS-pFGF2-EGFP) and core-shell-structured particles (HBC@TACS-pFGF2-EGFP) were prepared to explore the release regulation of pFGF2-EGFP of these particles. The expression of FGF2 protein was detected by Western Blot after in vitro transfection of 293T cells. Eighteen New Zealand white rabbits were used for adipose tissue transplantation experiment. The left ears were treated as experimental group and the right ears were treated as control group. The ears in experimental group were implanted with 2 mL fat particles and HBC@TACS-pFGF2 particles while the ears in control group were implanted with 2 mL fat particles and HBC@TACS-empty plasmids. The new adipose tissues were harvested to analyze the survival rate at 4, 8, 12 weeks after transplantation. Besides, the biological characteristics of grafts and the density of new blood vessels were observed at the same time by HE staining and immunohistochemistry. Results The FGF2 protein can be slowly released by HBC@TACS-pFGF2 particles in vitro and be successfully expressed after transfection of 293T cells. At different time point, new fat tissues were observed. The volume of adipose tissues were gradually reduced after transplantation. The survival rates of adipose tissues in the experimental group were significant higher than in control group at any time point (P<0.05). HE staining showed the new fat cells arranged more regularly in experimental group than the cells in control group. Immunohistochemistry staining showed the density of new blood vessels in adipose tissue in experimental group was higher than that in control group (P<0.05). Conclusion HBC@TACS-pFGF2-EGFP particles can improve the survival rate of fat transplantation. Related Articles | Metrics

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Effectiveness of Post-operative Superficial X-Ray Radiotherapy for Keloid GU Weixiang,CHEN Libin,CHANG Zhengwu 2018, 14 (5):  258-265.  DOI: 10.3969/j.issn.1673-0364.2018.05.005 Abstract ( 766 )   Objective To investigate the efficacy of postoperative superficial X-ray radiotherapy for keloids. Methods From April 2016 to April 2017, a total of 172 patients who underwent surgical resection of keloids were enrolled (263 lesions), and were given postoperative superficial X-ray radiotherapy within 24 hours. Each patient got 5 Gy/fraction/day for 4 days. Effectiveness, adverse reactions and recurrence rates were observed and evaluated. Results The total effective rate of the superficial X-ray radiotherapy was 92.8% (244/263). According to the lesion area analysis, the total effective rate of face and neck area, trunk area and limb area was 94.6% (106/112), 91.5% (86/94), and 91.3% (52/57) respectively. No serious adverse reactions were observed. Conclusion Surgical resection combined with superficial X-ray radiotherapy is a safe and effective method for keloid treatment. Related Articles | Metrics

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Nonsurgical Correction of Congenital Ear Deformities ZHANG Shuai,LI Jun,LIU Xiangqi,DONG Chenbin 2018, 14 (5):  260-262.  DOI: 10.3969/j.issn.1673-0364.2018.05.006 Abstract ( 368 )   Objective To explore the effectiveness of the nonsurgical correction of congenital auricular deformities. Methods A retrospective was performed on 28 infants (34 ears) with congenital auricular deformity who were treated with the ear molding system from July 2017 to December 2017. The average age at initiation of treatment was 37.62 days (12~105 d). Doctors who were not involved in treatment and parents carried out a survey one month after treatment. All cases were divided into two groups according to the age: <6 weeks group and ≥6 weeks group, and the effectiveness of the two groups was analyzed and compared. Results Thirty-four infant ear deformities were treated with the ear molding system. The mean duration of treatment was 33.88 days (18~59 d). Thirty-one ears (91.2%) and 3 ears (8.8%) were rated as very satisfied or satisfied by doctors who were not involved in treatment; Twenty-nine ears (85.3%) and 5 ears (14.7%) were rated as very satisfied or satisfied by parents. There was no significant difference between the<6 weeks group and ≥6 weeks group (P>0.05). Complications included minor superficial excoriation (n=1) and rashes (n=2). Conclusion The nonsurgical treatment of the ear molding system is effective in treating auricular deformities in 3 months of age, especially for newborn ear deformities. Related Articles | Metrics

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Effectiveness of Modified Watte Flap in Repairing Palatal Fistula after Maxillary Fracture ZHANG Yong,YANG Chengshuai,ZHANG Chuxi,HUI Wenyu,LIU Nian,ZHANG Shilei 2018, 14 (5):  263-265.  DOI: 10.3969/j.issn.1673-0364.2018.05.007 Abstract ( 314 )   Objective To evaluate the effectiveness of modified watte flap in repairing palatal fistula after maxillary fracture. Methods From 2015 to 2017, 10 patients with palatal fistula after maxillary fracture were treated by modified wat-te flap under general anesthesia. Results All wounds healed well. All patients were followed up for 1-6 months, the palate was well formed with no functional abnormality. Conclusion Modified watte flap is a simple and practical method to repair palatal fistula after maxillary fracture with less surgical injury. It can achieve good results in selected cases. Related Articles | Metrics

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Application of Simultaneous Breast Augmentation after the Resection of the Benign Breast Lesions under Endoscope CHEN Jing,WANG Shaoqiang 2018, 14 (5):  266-268.  DOI: 10.3969/j.issn.1673-0364.2018.05.008 Abstract ( 344 )   Objective To explore the feasibility of simultaneous breast augmentation after the resection of the benign breast lesions under endoscope. Methods From May 2013 to June 2017, 43 patients with benign breast lesions were treated. According to the patient's condition, the extent of breast lesion removal was marked, the range of the needed dissection to implant the prosthesis was designed, and the suitable prosthesis was chosed. The retromammary space were established under endoscope through transaxillary incision. Benign breast lesions were located and removed by puncture guiding technique. Silicone implants were placed through the axillary incision. The vacuum drainage was performed for 3 to 6 days. Results All operations were successfully completed. The operative time was (113.6±20.4) min, blood loss was (36.6± 4.8) mL, postoperative drainage was used for (3.4±1.2) days, unilateral breast drainage was (216.5±21.7) mL. Patients were followed up for 12 months, no significant nipple and areola sensory loss were observed. Two cases appeared mild local dent on the surface of the breast, 1 case appeared capsular contracture of Baker Ⅱlevel. The breast of the remaining patients was plump and round in shape with soft hand-feel and good activity. No prosthesis displacement, bimodal breast shape and other complications were observed. Conclusion For patients with benign breast lesions, simultaneous breast augmentation after the resection of the breast lesions under endoscope is safe and feasible. Related Articles | Metrics

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Application of Miniature Stent Combining Hand Orthosis in the Prevention and Treatment of Thumb Web Contracture YE Huiping,Wang Yingqiong,GAO Junqing,ZHAGN Jiasheng,FU Jile 2018, 14 (5):  269-294.  DOI: 10.3969/j.issn.1673-0364.2018.05.009 Abstract ( 392 )   Objective To explore the clinical effect of miniature stent combining hand orthosis in the prevention and treatment of thumb web contracture. Methods From February 2013 to October 2014, 42 cases with thumb web contracture were selected and randomly divided into 4 groups according to different treatments: stent & orthosis treatment group, stent treatment group, orthosis treatment group and conventional treatment group. After 16 weeks of follow-up, the width and angle of thumb web were observed and recorded. The superiority of each scheme was analyzed statistically. Results The width of thumb web increased (24.1±5.8) mm, (9.6±4.6) mm, (15.8±1.9) mm, (4.0±5.4) mm in stent & orthosis treatment group, stent treatment group, orthosis treatment group and conventional treatment group respectively 16 weeks after operation, and the differences among 4 groups were statistically significant (P<0.05). The angle of thumb web increased (23.5±6.1)°, (9.6±4.6)°, (15.7±1.9)°, (3.9±5.4)°in stent&orthosis treatment group, stent treatment group, orthosis treatment group and conventional treatment group respectively 16 weeks after operation, and the differences among 4 groups were statistically significant (P<0.05). Expect for one case of pinhole infection which cured after dressing change, no adverse reaction was observed. Conclusion Miniature stent combining hand orthosis for the prevention and treatment of thumb web contracture can effectively increase the width and angle of thumb web with less adverse reactions. It is worthy of popularization and application. Related Articles | Metrics

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Application of Platysma Musculocutaneous Flap in the Repair of Maxillofacial Trauma LIU Li,LIU Hongchao 2018, 14 (5):  272-274.  DOI: 10.3969/j.issn.1673-0364.2018.05.010 Abstract ( 447 )   Objective To explore the clinical efficacy of platysma musculocutaneous flap in the repair of maxillofacial trauma. Methods A total of 125 patients with maxillofacial trauma treated in our hospital from June 2015 to December 2017 were retrospectively analyzed. Sixty -three patients were treated with platysma musculocutaneous flap as the experimental group, while 62 patients were treated with anterolateral thigh musculocutaneous flap as the control group. The operation time, intra-operative blood loss, hospitalization days, donor wound healing time, wound healing time, maxillofacial function score and complication rate were compared between the two groups. Results The operation time, intra-operative blood loss and wound healing time of the donor side in the experimental group were significantly lower than those in the control group (P<0.01). There was no significant difference in the length of hospital stay and wound healing time between the two groups (P>0.05). The score of maxillofacial function in the experimental group was significantly higher than that in the control group (P<0.01). The skin flap necrosis rate (3.17% vs. 11.29%) and the complication rate (12.70% vs. 24.9%) of the experimental group were both significantly lower than that of the control group (P<0.05). Conclusion Repairing the maxillofacial trauma with the platysma musculocutaneous flap can significantly reduce the intra-operative risk, improve the prognostic function of the maxillofacial region and reduce complications. It is worthy of clinical promotion. Related Articles | Metrics

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Auto-Transplantation of Granulated Orbital Septum Fat for Correction of Tear Trough Deformity GAO Hui,LIU Chao,WU Xiaohui,LI Yan,XIE Miao,GUO Li,KANG Yanling,MIAO Menghan,YANG Jun 2018, 14 (5):  275-276.  DOI: 10.3969/j.issn.1673-0364.2018.05.011 Abstract ( 322 )   Objective To explore the clinical effect of auto-transplantation of granulated orbital septum fat for correction of tear trough deformity. Methods From October 2015 to October 2017, 60 patients with pouch, tear trough deformity and lower eyelid skin relaxation were selected in this study. The orbital septum fat was released and removed by lower eyelid myocutaneous flap, and then was transplanted into tear trough after being cut into tiny particles. The excess skin and musculi dormitator were removed after transplantation. Results All patients were followed up for 6 months. Except for one patient who had been filled twice due to the deficiency of orbital septum fat, all eyelid pockets disappeared, the tear tough and the surrounding wrinkles were improved in appearance, and the lower eyelid area was flat and tight, without bump and no touch of nodules. Conclusion Transplantation of granulated orbital septum fat has satisfactory effect for correction of tear trough deformity and lower eyelid skin relaxation in pouch patients, the survival rate of fat is high. The operation is worth popularizing. Related Articles | Metrics

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The Efficacy and Safety of Fractional Laser Technology in Facial Rejuvenation CAI Yuanyuan,JING Changrui,LI Yongzhong 2018, 14 (5):  277-279.  DOI: 10.3969/j.issn.1673-0364.2018.05.012 Abstract ( 424 )   Objective To explore the effects and safety of fractional laser technology in facial rejuvenation. Methods From May 2016 to May 2017, 60 cases with facial skin aging were selected and were treated with fractional laser technology, the therapeutic effects were evaluated. Results There were 46 cases with obvious therapeutic effect, 13 cases with effective effect and 1 case with invalid effect. The total effective rate was 98.33%. The shape of nasolabial folds, face and eyebrows were significantly improved after 2 months of treatment. Furthermore, the lift degree of nasolabial folds, face and eyebrows after 6 months of treatment were significantly higher than that after 2 months of treatment (P<0.05). VAS score immediately after treatment was (2.65±1.01). Treatment overall satisfaction rate of patients was 96.67%. No scar, infection, pigmentation, blisters, pimples and other significant adverse reactions occurred. Conclusion Fractional laser technology for facial rejuvenation is effective with fewer side effects. It is a safe and effective treatment method for facial rejuvenation. Related Articles | Metrics

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Electromyography Analysis of Masseter Muscles after Mandibular Angle Osteotomy BIAN Jie,MENG Zhibing,HUANG Jinhua,GUO Jun 2018, 14 (5):  280-281.  DOI: 10.3969/j.issn.1673-0364.2018.05.013 Abstract ( 312 )   Objective To explore the changes of masseter muscle function after mandibular angle osteotomy through electromyography analysis. Methods From October 2010 to June 2015, 18 patients who received mandibular angle osteotomy were selected. The masseter electromyogram was analyzed before operation, one month and one year after operation. The difference was statistically analyzed. Results Compared with the masseter electromyogram before operation, the masseter electromyogram one month after operation was significantly decreased (P<0.05). While there was no significant difference between the masseter electromyogram one year after operation and that before operation (P>0.05). Conclusion The masseter function can bebasically recovered one year after mandibular angle osteotomy. Related Articles | Metrics

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Application of Conscious Sedation with Dexmedetomidine and Sufentanil in Liposuction Surgery FANG Shudong,ZHOU Chi,ZHI Yankang,SUN Yu,JIANG Hong 2018, 14 (5):  282-284.  DOI: 10.3969/j.issn.1673-0364.2018.05.014 Abstract ( 377 )   Objective To observe the effectiveness and safety of conscious sedation with dexmedetomidine and sufentanil in liposuction surgery. Methods From January 2018 to June 2018, 40 female patients (ASA Ⅰ-Ⅱ) scheduled for liposuction surgery were included. The patients were infused l μg/kg intravenously within 10minutes as loading dose followed by a continuous infusion of dexmedetomidine 0.4μg/Kg·h-1. Sufentanil 0.2 μg/Kg were administrated intravenously before infusion of tumescent solution. Sufentanil 5-10 μg was given increased because of movement caused by surgical stimulation. The drug infusion was discontinued at 5 to 10 min before the end of the surgical procedure. Hemodynamics, respiratory changes, Ramsay Sedation Score (RSS) were observed and registered at the time point of before sedation, 15, 30, 60 min after sedation and the end of the surgery. The complications (anoxemia, apnea, bradycardia, etc), anesthesia duration and drug consumption were recorded. Results All patients were satisfied with the conscious sedation (Ramsay sedation level: 2~4). The induction of sedation produced a significant decrease in HR (P<0.05). The SBP, DBP were significantly decreased 15 minutes after sedation, compared with pre-sedation (P<0.05). There were no significant differences in SBP and DBP at other time points after sedation compared with pre-sedation (P>0.05). There were no significant changes in RR and SpO2after sedation (P>0.05). Hypoxia and bradycardia were observed in 2 patients respectively. No serious adverse reactions occurred. The anesthesia duration and consumption of dexmedetomidine, sufentanil were (168±58) min, (126±36) μg, (15±5) μg respectively. Conclusion Conscious sedation with dexmedetomidine and sufentanil is an effective and safety anesthetic technique in patients for liposuction surgery. Related Articles | Metrics

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Effectiveness of Extended Nursing Intervention on Improving Health Outcomes of Children with Cleft Lip and Palate Huang Ying,HU Jiahua 2018, 14 (5):  285-287.  DOI: 10.3969/j.issn.1673-0364.2018.05.015 Abstract ( 339 )   Objective To explore the effectiveness of extended nursing intervention on improving health outcome of children with cleft lip and palate. Methods From February 2017 to February 2018, 90 children received cleft lip and palate treatment were selected, and were divided into control group and experimental group according to a convenient grouping method. There were 45 cases in each group. Children in control group were treated with conventional nursing method, while children in experimental group were treated with extended nursing intervention method. Results The incidence of postoperative complications, the SDS and SAS scores in the experimental group were all lower than in the control group (P<0.05). The degree of satisfaction in family nursing care in the experimental group was higher than in the control group (P<0.05). Conclusion The application of extended nursing intervention on children with cleft lip and palate can reduce the probability of postoperative complications, improve their family's nursing care ability, and is worthy of clinical promotion. Related Articles | Metrics

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Design and Clinical Application of a Comic-style Health Education Pamphlet on Infants' Postoperative Hand Care YANG Jiafei,BIAN Weiwei,WANG Hairong,LU Wei,DONG Liping 2018, 14 (5):  288-290.  DOI: 10.3969/j.issn.1673-0364.2018.05.016 Abstract ( 338 )   Objective To design a comic-style health education pamphlet on infants' postoperative care after hand surgery, and to investigate its clinical application. Methods Sixty child patients who received reconstructive hand surgery were included. In accordance with the random number table, the patients were divided into 2 groups with thirty patients each:experimental group and control group. The control group was treated with traditional oral health education, while the experimental group was introduced an additional comic-style pamphlet regarding postoperative care. Parents' feedbacks of two groups were compared and analyzed. Results The parents of the experimental group mastered the contents of postoperative education significantly better than the control group (P<0.05), and the difference was statistically significant. Conclusion Compared with traditional oral-only health education, the comic-style education pamphlet is a better way to enhance parents'understanding and memorizing of postoperative care. Related Articles | Metrics

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Treatment Progress of Dermatofibrosarcoma Protuberans ZHOU Xiaobo,YANG Jun,LUO Xusong 2018, 14 (5):  291-294.  DOI: 10.3969/j.issn.1673-0364.2018.05.017 Abstract ( 518 )   Dermatofibrosarcoma protuberans (DFSP) is a rare soft tissue sarcoma. Its high recurrence rate is a clinical challenge. The complete resection of the sarcoma with the maximum reservation of normal tissue is the key to the treatment. The three-dimensional pre-operative reconstruction technique has been applied into many kinds of tumor. It contributes greatly to operative planning and precising resection of tumor. In this paper, the treatment progress of DFSP and the application prospects of 3D pre-operative reconstruction technique in DFSP were discussed, providing a new idea for the precise treatment of DFSP. Related Articles | Metrics

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Advances of the Long-Term Preservation of Adipose Tissue ZHANG Peiqi,ZHOU Shuangbai,LI Qingfeng 2018, 14 (5):  295-297.  DOI: 10.3969/j.issn.1673-0364.2018.05.018 Abstract ( 317 )   Since the use of liposuction in 1980s, adipose tissue has become a common material on soft tissue repair. However, multiple fat transplantations are still necessary for clinical use. Hence the proper methods of the adipose tissue preservation after a single liposuction enable the reinjections on proper time, which can eliminate the repeated liposuction and reduce the pain of patients. Thus it is critical to preserve the adipose tissue and improve the retention after fat transplantation. In this paper, the advances of the long-term preservation of adipose tissue were reviewed. Related Articles | Metrics

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Research Progress of Nano-Fat in Autologous Fat Transfer KANG Deni,QIAN Chuanmu,LUAN Jie 2018, 14 (5):  298-300.  DOI: 10.3969/j.issn.1673-0364.2018.05.019 Abstract ( 341 )   Autologous fat transplantation (AFT) is an indispensable method in the field of plastic surgery, which has many advantages such as alternative sources, availability, biological compatibility and minor surgical trauma, but high absorption severely limits its development. Nano-fat is a research hotspot in recently and plays an important role in tissue regeneration and anti-aging. In this paper, the research progress of nano-fat in autologous fat transfer was reviewed. Related Articles | Metrics