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The effect of extracellular vesicles secreted by adipose-derived mesenchymal stem cells after being stimulated by ultrasound on the biological properties of adipose-derived mesenchymal stem cells LIU Zibo, WANG Yikai, ZHENG Yi, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 403-.   Abstract （738）      PDF（pc） （5906KB）（25）       Knowledge map    Save Objective　To collect extracellular vesicles（ EVs） secreted by adipose mesenchymal stem cells（ ADSCs） after ultrasound stimulation， compare with EVs secreted by normal ADSCs， and to explore the effects of EVs secreted after ultrasound stimulation on the biological characteristics of ADSCs， including cell proliferation， migration， and adipogenic differentiation abilities. Methods　Firstly， some ADSCs were treated with low-intensity ultrasound， cell culture supernatant was collected and extracellular vesicles （U-EVs） were extracted by ultrafiltration method. Some ADSCs were cultured normally and their extracellular vesicles（ N-EVs） were also collected. The experiments were performed in the following three groups： PBS （blank control group）， EVs secreted by untreated cells （N-EVs group）， and EVs secreted by ultrasoundtreated cells （U-EVs group）. The CCK-8 assay was used to assess the viability of ADSCs under treatment with EVs at different concentrations. The concentration that most strongly enhanced cell viability was selected. At this concentration， cell migration was detected by scratch test， cell proliferation was detected by EdU method， and adipogenic induction medium was used to induce and compare adipogenic differentiation ability of ADSCs. Results　EVs secreted after ultrasound stimulation met the identification standards， and the optimal concentration for affecting ADSCs was about 150 µg/mL. At this concentration， the cell proliferation rate of the U-EVs group（ 19.2%±1.8%） was significantly higher than that of the N-EVs group（ 11.9%±3.4%）， and the Oil Red O staining area of the U-EVs group was（ 2.78±0.23） times that of the control group， significantly higher than （2.11±0.18） times of the N-EVs group. However， there was no significant difference in cell migration rates between the N-EVs （16.3%±2.0%） and U-EVs （21.5%±2.3%） groups. Conclusion　 EVs secreted by ultrasound-stimulated ADSCs significantly enhance the vitality of ADSCs and improve their abilities in cell proliferation， migration， and adipogenic differentiation. Related Articles | Metrics | Comments（0）

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Application of head-mounted video system in the teaching of free flap reconstructive surgery ·CHENG Chen, CHENG Kaixiang, SHAO Jing, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 500-.   Abstract （570）      PDF（pc） （941KB）（11）       Knowledge map    Save Objective　To evaluate the effect of head-mounted video system in the teaching of free flap reconstructive surgery. Methods　A total of 45 surgeons with further study program in the Department of Plastic and Reconstructive Surgery of Shanghai Ninth People's Hospital from June 2022 to December 2023 were included and randomly divided into experimental group （n=23） and control group （n=22）. Both groups were given lectures about basic theoretical knowledge and surgical procedure before operation. The experimental group observed the surgery by video system， and the video data was directly taken from the head-mounted video device of the operator and accompanied by explanation， while the control group was observed in the operation room. The basic theoretical assessment， surgical procedure assessment and teaching satisfaction rate were compared between the two groups. Results　The scores of surgical procedure assessment and teaching satisfaction rate of experimental group were higher than those of control group， the difference was statistically significant（ P<0.05）， but there were no statistically significant difference in the basic theoretical assessment scores between the two groups （P>0.05）. Conclusion　The head-mounted video system can improve the teaching effect and teaching satisfaction of flap reconstructive surgery， which has certain promotion significance. Related Articles | Metrics | Comments（0）

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Hypodermic needle guided suturing for distant tension-offloading suture PAN Sida, WU Qinghua, PENG Qian, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 456-.   Abstract （519）      PDF（pc） （1487KB）（13）       Knowledge map    Save Distant tension-offloading suture is one of the solutions for tension impeded wound healing and scarring after surgery. In this paper， an innovated technique of achieving tension-offloading suturing using hypodermic needle guidance was introduced. Hypodermic needle guided suturing（ HGS） technique makes high quality wound closure simple， with cheap and accessible equipment. Related Articles | Metrics | Comments（0）

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Advances in minimal-invasive blepharoplasty YANG Baofeng, LI Jie, MA Jiguang Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 491-.   Abstract （511）      PDF（pc） （969KB）（17）       Knowledge map    Save The application of minimal-invasive blepharoplasty has been increasing year by year because of its advantages such as invisible surgical scar， stable and natural double eyelid and short recovery period. This review summarized the advances of minimal-invasive blepharoplasty at home and abroad， and introduced the surgical procedure of minimal-invasive blepharoplasty and the advantages， disadvantages and complications， so as to provide reference for the clinical application of minimal-invasive blepharoplasty. Related Articles | Metrics | Comments（0）

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Progress in research methods of studying the neural crest JIANG Leheng, YIN Ningbei Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 469-.   Abstract （471）      PDF（pc） （993KB）（101）       Knowledge map    Save The neural crest is a migratory， multipotent stem cell population during vertebrate embryogenesis， and lineage tracing is the focus of its research. Over the 150 years since its first discovery， understanding of the neural crest has gone through a transition from morphological observations to single-cell level. In this paper， important approaches applied to study the neural crest were reviewed including radioisotope labeling， chimeras， vital dye labeling， in vitro clonal analysis， conditional genetic recombination and single-cell sequencing. Related Articles | Metrics | Comments（0）

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modRNA enhance the effect of cell therapy in tissue repairing and regeneration ·WANG Xiangying, FU Wei Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 465-.   Abstract （415）      PDF（pc） （976KB）（20）       Knowledge map    Save 　Cell therapy has been developed rapidly in recent years. However， the effect of cell therapy in tissue repairing and regeneration is ineffective. Therefore， a new strategy to enhance the therapeutic effect of cell therapy is urgently demanded. Some studies in recent years have found that the combination of mRNA with cell therapy， that is， transfect mRNA into transplanted cells， can promote the efficiency of tissue repairing and regeneration， which could further promote the repair and regeneration of multiple tissues such as cardiovascular， skin， bone and articulus. In this paper， the advantages of the combination of mRNA with cell therapy， other applications in tissue repairing and regeneration， and the difficulties of clinical transformation were all reviewed， aiming to provide new ideas for the tissue repairing and regeneration and the strategies of the clinical transformation. Related Articles | Metrics | Comments（0）

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Clinical effects and prognosis of oral sirolimus in the treatment of abdominal lymphatic malformations XU Zhenguo, CHEN Tao, TAI Maozhong, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 434-.   Abstract （364）      PDF（pc） （9690KB）（12）       Knowledge map    Save Objective　 To observe the clinical effect and prognosis of oral sirolimus in the treatment of abdominal lymphatic malformations. Methods　From March 2020 to August 2022， clinical data of 5 patients with abdominal lymphatic malformations treated with oral sirolimus were retrospectively analyzed. Results　 All patients had abdominal pain before admission and were treated with oral sirolimus for 2 years. Subjective evaluation of clinical symptom improvement： Progress in 0 case， stability in 2 cases， improvement in 3 cases， cure in 0 case. Objective evaluation of lesion improvement： Grade Ⅰ（poor） in 2 cases， grade Ⅱ（ medium） in 0 cases， grade Ⅲ（ good） in 1 case， grade Ⅳ（ excellent） in 2 cases. During the treatment， 2 patients developed oral ulcers and 1 patients developed mild anemia， all of which were improved after symptomatic treatment. All patients had no serious complication. After 1 year of follow-up， abdominal pain and other symptoms did not appear again， and the tumors of 3 cases （macrocystic lymphatic malformations） were significantly reduced， and 2 cases （microcystic lymphatic malformations） did not show significant tumor growth. Conclusion　Sirolimus is safe and effective in the treatment of abdominal lymphatic malformations， and can significantly relieve abdominal discomfort symptoms， especially for macrocystic lymphatic malformations， with significant tumor size reduction and satisfactory results.  Related Articles | Metrics | Comments（0）

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Constructing a quality evaluation index system based on Donabedian theory for surgical care in plastic and reconstructive surgery specialties QIAN Fuwen, YANG Yuelai, ZHU Yonggan Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 447-.   Abstract （328）      PDF（pc） （968KB）（39）       Knowledge map    Save Objective　To establish the evaluation index system of nursing quality of plastic and reconstructive surgery specialties based on Donabedian theory. Methods　 Thirty five experts from Three-A hospitals in China in plastic and reconstructive surgery were consulted through email from April to June in 2023. The primary nursing quality evaluation index system of plastic and reconstructive surgery specialties was drawn up through literature research and qualitative interview methods. Two rounds of expert consultation were conducted by Delphi method to establish the nursing quality evaluation index system of plastic and reconstructive surgery specialties. Results　The composition of experts in the two rounds of consultation was consistent， and the Cr of the experts was 0.777 1. In this study， the coefficient of variation of the secondary and tertiary indexes was 0.107-0.129， and the coefficient of variation was below 0.15， indicating that the two round consultations were credible and the coordination of the experts was good. Eventually， experts were consistant with each other in regard to all the indexes， the evaluation indexes of nursing quality in plastic and reconstructive surgery including 3 primary indexes， 10 secondary indexes and 47 tertiary indexes were determined through two round consultations by email. Conclusion　 This study established a relatively scientific， rigorous and comprehensive evaluation index system for the quality of surgical care in plastic and reconstructive surgery， providing a theoretical basis for optimizing the quality of nursing care in plastic and reconstructive surgery and improving the satisfaction of patients and doctors. Related Articles | Metrics | Comments（0）

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Research progress of intramuscular fatty infiltration LU Baixue, GAO Weicheng Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 481-.   Abstract （286）      PDF（pc） （970KB）（62）       Knowledge map    Save Fat infiltration plays an important role in the pathological development of muscle related diseases， and is closely related to disease recurrence and poor prognosis. In recent years， the research on the potential mechanism of intramuscular fat infiltration has been highly popular at home and abroad， mainly focusing on the basic and clinical research related to skeletal muscle and myocardial fat infiltration. In this paper， the relevant literature on the pathogenesis of intramuscular fat infiltration in skeletal muscle and myocardium in recent years was reviewed， to provide assistance for the basic and clinical research of intramuscular fat infiltration related diseases. Related Articles | Metrics | Comments（0）

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Neuroprotective effect of Liraglutide（LRG）on spinal cord injury（SCI）in rats by inhibiting ferroptosis DING Shuai, ZHANG Guangquan, GAO Kun, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 422-.   Abstract （281）      PDF（pc） （2406KB）（18）       Knowledge map    Save Objective　To observe the neuroprotective effect of liraglutide（ LRG） on spinal cord injury（ SCI） rats， and to explore whether its mechanism is related to inhibiting ferroptosis. Methods　A total of 90 female Wistar rats were randomly divided into sham group（ n=30）， SCI group（ n=30） and LRG+SCI group（ n=30）. A rat model of SCI was established using the modified Allen’s method. For the SCI+LRG group， LRG （200 µg/kg） was administered immediately via subcutaneous injection（ s.c.） following SCI， and then subsequently given once a day for the next ten days. Animals in both the sham and SCI group were administrated with equal volumes of sterile PBS. The iron content and glutathione（ GSH） level in spinal cord were detected by the kit at 24， 48 and 72 h after operation. The expression levels of xCT and GPX4 protein were analyzed by Western blot. The BBB locomotion rating scale was used to investigate the hindlimb function 1， 3， 7， 14， 28 days after injury. HE staining and Nissl Staining were performed to evaluate the structural damage and surviving neurons around the injured area 28 days after operation， and the neuron apoptosis was detected by immunofluorescence staining. Results.　Compared with the sham operation group， the iron content in SCI group was significantly increased within 3 days after injury （P<0.05）， and GSH level was significantly decreased （P<0.05）. LRG treatment effectively decreased the iron contents in injured spinal cords（ P<0.05）， and increased GSH level （P<0.05）. In addition， LRG treatment significantly increased the expression levels of xCT and GPX4 in injured spinal cords（ P<0.01）. Compared with SCI group， the BBB score of LRG group was significantly higher at 7， 14 and 28 days after injury （P<0.05）. HE staining and Nissl staining showed that cavity of damaged regions of LRG group was lower than that of SCI group （P<0.05）， and the number of motoneurons in the spinal ventral horn of rat treated with LRG was higher than that of SCI group （P<0.05）. Immunofluorescence staining showed that the ratio of cleaved-caspase 3-positive neurons was remarkably increased after SCI（ P<0.05） and LRG significantly reduced the ratio of cleaved-caspase 3-positive neurons（ P<0.05）. Conclusion　LRG treatment after SCI may promote SCI repair， protect damaged neurons and restore motor function through inhibiting ferroptosis in the microenvironment at the lesion site. Related Articles | Metrics | Comments（0）

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The application and thinking of problem enriched instruction in clinical teaching of dermatology and venereology based on psychology of learning ·HUANG Linting, LIU Lanjun, WANG Xue, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 496-.   Abstract （264）      PDF（pc） （967KB）（17）       Knowledge map    Save Based on psychology of learning， this study analyzed the conditions and levels of problem enriched instruction from the perspective of learning condition theory and problems， summarized the characteristics of various problems， and the teaching design of the chapters of“ herpes zoster” in the textbook of dermatology and venereology was performed， aiming to broaden students’ understanding of herpes zoster， and try to improve students’ understanding through multiple dimensions such as the clinical professional competence， medical exploration and medical ideological and political education through the layer-by-layer analysis based on the minimum problem unit. Related Articles | Metrics | Comments（0）

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Advancements of fractional photoelectric technology in periorbital rejuvenation YAN Li, CHEN Xiaoying, PAN Meng Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 486-.   Abstract （258）      PDF（pc） （981KB）（11）       Knowledge map    Save The periorbital region is one of the earliest areas to show signs of aging， and its aging process can effectively reflect a person's level of aging. Periorbital aging involves various issues such as dark circles， wrinkles， tear troughs， and under-eye bags， which can give people a tired， depressed， or angry appearance. Fractional photoelectric technology is widely used in periorbital rejuvenation as it adopts the principle of fractional photothermolysis， which can form dozens of microscopic treatment zones， stimulating collagen regeneration and enabling rapid repair of the surrounding normal skin tissues. In this paper， the application of fractional photoelectric technology in periorbital rejuvenation was reviewed， aiming to provide references for clinical practice. Related Articles | Metrics | Comments（0）

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Selection of vascular anastomosis methods for breast reconstruction using lobed free lower abdominal flap transplantation SONG Dajiang, ZHANG Tianyi, WANG Zhiyuan, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 428-.   Abstract （217）      PDF（pc） （9081KB）（19）       Knowledge map    Save Objective　To summarize the indications and vascular anastomosis methods selection for the transplantation of lobed free lower abdominal flap for breast reconstruction. Methods　 From October 2019 to June 2023， 17 female breast cancer patients received lobulated free lower abdominal flap transfer for breast reconstruction， including 10 cases of immediate breast reconstruction and 7 cases of delayed breast reconstruction. All the cases were completed by transplantation of lobulated free lower abdominal flaps， including 20 M1 type free rectus abdominis skin flaps， 6 M2 types， and 8 M3 types. The specific vascular anastomosis methods include supercharge in 9 cases and turbocharge in 8 cases. The recipient vessels include 14 sides of the proximal end of the internal mammary vessels， 10 sides of the main trunk of the thoracodorsal vessels， 3 sides of the serratus anterior branches， 2 cases of the lateral thoracic artery and vein， and 5 sides of the distal end of the internal mammary vessels. Results　 Each side flap had a length of （14.2±0.4） cm， a width of （11.5±1.0） cm， and a thickness of（ 4.9±0.3） cm. All flaps had good blood circulation and all survived smoothly. All patients were followed up for 6 to 38 months （20.7 months on average）， no recurrence was noted. The reconstructed breasts had good appearance， satisfactory texture， no flap contracture and deformation， and was basically symmetrical with the healthy breast. The flap donor site and breast recipient area only had linear scars left， and there was no significant impact on abdominal wall function.Conclusion　The lobed free lower abdominal flap is suitable for breast reconstruction with large base and sagging convexity. Choosing a reasonable vascular anastomosis method can ensure the free placement of the flap and satisfactory breast shaping. Related Articles | Metrics | Comments（0）

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Research progress on the mechanism of skin aging caused by ultraviolet radiation CHEN Heng, WANG Jue, ZHOU Xu Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (3): 382-.   Abstract （212）      PDF（pc） （992KB）（2098）       Knowledge map    Save Skin exposed to sunlight for a long period of time will have impaired function and even genetic material alteration， which is manifested by aging characteristics such as water loss， elasticity loss， wrinkle growth， melanin deposition and dryness. There are many ways to cause skin photoaging and the mechanism is complex， the ultraviolet light in sunlight has a particularly obvious effect on skin aging， the ultraviolet light that can act on the skin is divided into two kinds： UVA and UVB. They mainly act on the skin through five mechanisms that lead to photoaging： oxidative stress， damaging genetic material， changing the epidermal microbial community， destroying the function and structure of organelles， and reshaping the extracellular matrix （ECM）.In this paper， these five mechanisms were reviewed， and research directions and prospects in this field were proposed. Related Articles | Metrics | Comments（0）

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Clinical application value of spinal robot assisted system in MIS-TLIF surgery LIU Yang, LIU Daokuo, LI Changkuan, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (3): 300-.   Abstract （180）      PDF（pc） （3575KB）（66）       Knowledge map    Save Objective To study the clinical application value of spinal robot assisted system in minimally invasive transforaminal fusion （MIS-TLIF） surgery. Methods From March 2019 to November 2022，78 patients who underwent surgical treatment for lumbar degenerative diseases were divided into traditional MIS-TLIF group （group A，41 cases） and robotic MIS-TLIF group （group B，37 cases） according to different surgical methods. Perioperative indicators （intraoperative bleeding， surgical time， incision length， and fluoroscopy frequency）， postoperative CT findings （screw placement accuracy，screw abduction angle）， functional recovery （VAS， JOA scores）， and perioperative complications were compared between the two groups. Results The intraoperative bleeding volume and fluoroscopy frequency in Group B were less than those in Group A， and the operative time and incision length were shorter than those in Group A （P<0.05） . A total of 164 screws were implanted in Group A，148 in Group B. The acceptable screws in Group B were significantly higher than those in Group A （P< 0.05） . The abduction angle of screws in Group A was 18.12°±7.50°， and that in Group B was 23.56°±6.64°. The abduction angle in Group B was greater than that in Group A （P<0.05） . One month after surgery， the VAS score of the two groupsdecreased compared to that before surgery， while the JOA score increased （P<0.05）.However， there was no statistically significant difference between the two groups （P>0.05）.There were no serious complications such as blood vessels and nerves in both groups； In group A，3 patient had a positioning error and 2 patient had a dural sac tear， all of which recovered after corresponding treatment； Group B had no early complications. The incidence of early complications in Group B was lower than  that in Group A （P<0.05）.Conclusion The spinal robot assisted system can shorten the operation time of MIS-TLIF，reduce intraoperative bleeding， and improve the accuracy of pedicle screw placement. It has high clinical application value. Related Articles | Metrics | Comments（0）

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Clinical study on the combined application of autologous epidermal cell suspension and meshed autologous split thickness skin graft in the treatment of deep partial thickness burns YANG Huizhong, XU Zhenpeng, YUAN Bo, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 411-.   Abstract （172）      PDF（pc） （5183KB）（25）       Knowledge map    Save Objective　To evaluate the effect of autologous epidermal cell suspension combined with mesh skin graft in deep II-degree burn wounds. Methods　Patients with deep partial thickness burns were selected as the research subjects and randomly divided into two groups， control group and experimental group， according to the random table method. Control group received split-thickness mesh skin graft（ n=8）， and experimental group received autologous epidermal cell suspension combined with split-thickness mesh skin graft（ n=8）. The epidermal cell suspension was prepared from autologous donor skin by cell separator. The wound healing time， wound healing rate on first week after treatment， and scar score （Vancouver scale） on 8th week after surgery were statistically analyzed in the two groups. At the same time， the concentration and viability of epidermal cells in the cell suspension were detected. Results　The healing time of experimental group was （10.2±0.3） days， compared with（ 12.1±0.6） days in control group（ P<0.05）. The healing rates of control group and experimental group were 76.97%±10.68% vs. 94.85%±10.01% （P<0.05） one week after treatment， and the Vancouver scar scores of control group and experimental group were 6.13±3.44 vs. 2.92±1.07 （P<0.05） at 8 weeks after surgery. The concentration of cell suspension was（ 1.77±0.11）×106 cells per cm² skin sample， and the cell viability was 87.85%±4%. Conclusion　This study preliminarily confirmed that autologous epidermal cell suspension combined with mesh skin transplantation in the treatment of deep partial thickness burns can promote skin grafting healing time， reduce donor area， and improve postoperative scar hyperplasia. Related Articles | Metrics | Comments（0）

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Low-level laser therapy for androgenetic alopecia ；A consensus among chinese experts Hair Medicine Branch of the Chinese Society of Aesthetic and Plastic Surgery, Chinese Non-government Medical Institutions Association Dermatology Branch Hair Medicine and Scalp Health Management Professional Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (2): 157-.   Abstract （167）      PDF（pc） （959KB）（260）       Knowledge map    Save Low-level laser therapy for androgenetic alopecia has been used clinically for over ten years， with definite efficacy and high safety. A variety of low-level laser devices have been approved and marketed domestically， and are widely used in clinical settings， yet there are currently no unified usage standards. This consensus compiled and summarized relevant guidelines and clinical research from both domestic and international sources in recent years， providing a reference for the correct selection and use of low-level laser therapy for androgenetic alopecia in clinical practice. Related Articles | Metrics | Comments（0）

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Experimental study of human umbilical cord mesenchymal stem cells on recovery of cavernosum nerve function in a rat model of diabetic-induced erectile dysfunction YANG Yang, LIU Fang, LI Shuaixiu, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (4): 395-.   Abstract （163）      PDF（pc） （6071KB）（28）       Knowledge map    Save Objective　 To explore the restoration effects of human umbilical cord mesenchymal stem cells on erectile function in rats by repairing and regenerating cavernous nerve. Methods　Umbilical cords of healthy full-term newborns were collected， human umbilical cord mesenchymal stem cells（ hUCMSC） were isolated and cell surface markers were identified. The rat model of diabetes-induced erection dysfunction （DIED） was established by intra-abdominal streptozotocin （STZ） injection in combination with high-fat diet， and an apomorphine（ APO） test was performed to screen diabetic rat model with erectile dysfunction after 10 weeks. Eight weeks after the transplantation of hUCMSC， erectile function was evaluated by APO test and maximum intracavernous pressure/mean arterial pressure （ICP/MAP） ratio. The levels of neuronal nitric oxide synthase （nNOS） in corpus cavernosum tissues of rats were analyzed by immunohistochemistry analysis. In addition， the expression levels of neurotrophic growth factors including BDNF， NGF， VEGF， and IGF-1 in the corpus cavernosum tissues of SD rats were detected by ELISA. Results　 The hUCMSC was successfully isolated from human umbilical cords， and results of flow cytometry showed that CD73， CD90 and CD105 were highly expressed while CD11b， CD19， CD34， CD45 and HLA-DR were low expressed on the surface of P4 hUCMSC. The rat model of diabetic-induced erectile dysfunction was successfully established. After 8 weeks of hUCMSC therapy， the results of APO test showed that hUCMSC enhanced the erectile function of DIED rats， as evidenced by an increase in the number of complete erection. Compared with the negative group， the blood flow in the penis was improved as indicated by obvious increases in ICP/MAP ratio values in medium-dose and high-dose groups of hUCMSC treatment. Immunohistochemistry results suggested that after the treatment of hUCMSC in medium and high doses， hUCMSC treatment significantly elevated the expression level of nNOS in the corpus cavernosum tissue of DIED rats， then induced penile erection in rats. Furthermore， ELISA test results indicated that the expression levels of BDNF， NGF， VEGF and IGF-1 could be restored in different degrees after treatment with medium- and high-dose of hUCMSC. Conclusion　hUCMSC is a kind of adult pluripotent stem cells， which is effective and dose-dependent in the treatment of DIED rats induced by high fat diet combined with STZ following intracavernosal transplantation. The mechanism of hUCMSC treatment is related to neuroprotective and regenerative repair effects provided by upregulation of the expression levels of BDNF， NGF， VEGF and IGF-1. Related Articles | Metrics | Comments（0）

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Clinical study of novel supramolecular salicylic acid technique in the treatment of acne rosacea HUANG Yan Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (2): 216-.   Abstract （161）      PDF（pc） （1822KB）（409）       Knowledge map    Save Objective To explore the efficacy and safety of 30% supramolecular salicylic acid in the treatment of acne rosacea. Methods A total of 165 patients diagnosed with rosacea from 2019 to 2021 were selected and randomly divided into control group and observation group. Among them， 85 patients in the observation group received traditional treatment combined with supramolecular salicylic acid and skin repair moisturizer. The control group of 80 patients was treated with traditional regimen. The therapeutic effect and incidence of adverse reactions were statistically analyzed in the two groups. Results Before treatment， there were no statistical differences in facial spots， red areas and pores between the two groups （P>0.05）， and after treatment， the above indexes were improved， and the observation group was better than the control group （P<0.05） . The total effective rate of the observation group was 97.65%， which was significantly higher than that of the control group （85%， P<0.05） . The incidence of adverse reactions in observation group was 17.65%， which was significantly lower than that in control group （35%， P<0.05）. Conclusion Supramolecular salicylic acid combined with traditional drugs has significant effect and low incidence of adverse reactions in the treatment of rosacea， and can be used as an adjuvant therapy for rosacea. Related Articles | Metrics | Comments（0）

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Discussion on talent cultivation of plastic surgery in the new era ZHANG Hanrui, HUANG Xin, LI Qingfeng, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (3): 388-.   Abstract （155）      PDF（pc） （927KB）（92）       Knowledge map    Save Plastic surgery is a specialized branch of surgery that has two main development directions： Reconstructive plastic surgery and aesthetic plastic surgery. In recent years， there has been a growing inclination among young doctors to prioritize aesthetic plastic surgery over reconstructive surgery， which has hindered the sustainable development of plastic surgery. Meanwhile， emerging technology has provided new opportunities for talent cultivation. The education model for young doctors should advance with time. Therefore， the recommendations such as strengthening medical history education， guiding correct understanding， and innovating teaching models， have been proposed to improve the quality and efficiency of talent cultivation. These efforts aim to cultivate highly qualified talents with strong professional skills and a sense of responsibility， who can adapt to the development needs of the discipline. Related Articles | Metrics | Comments（0）