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23 July 2020, Volume 12 Issue 6 Previous Issue    Next Issue

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Aesthetical Mini Wraparound Flap Supplied by Dorsal Digital Artery for Nail Bed Reconstruction GUO Shunu,LIU Mingbo,LU Youwen,LIU Liangyi,WANG Kelie,YANG Yanjun,ZHANG Ziqing 2016, 12 (6):  30-33.  DOI: 10.3969/j.issn.1673-0364.a463 Abstract ( 298 )   ObjectiveTo explore the surgical techniques and clinical effects of the mini wraparound flap supplied by the first dorsal digital artery for nail bed reconstruction.MethodsFrom Jan 2012 to Jan 2016,the mini wrap-around flap supplied by the first dorsal digital artery was harvested to reconstruct nail bed defect,including 12 cases in thumb,7 cases in the index finger,2 cases in the middle finger and 2 cases in ring finger.The size of the defects ranged from(6 mm×12 mm)－(14 mm×28 mm).The donor sites were directly closured,or covered with full-thickness skin grafts and pedicle flaps. ResultsAll the flaps were survived.Patients were followed up for 6－48 months.Twenty-three cases were all satisfied with near-normal appearance and good function.No significant morbidity was observed at the donor site and the function was not affected.ConclusionMini wraparound flap supplied by the first dorsal digital artery is an excellent method for entire or partial nail bed reconstruction. Related Articles | Metrics

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Labeling PLGA Scaffold with Gold Nanoclusters WANG Xiangsheng,WANG Xiansong,ZHANG Zhiyong,ZHOU Guangdong,LIU Wei,CAO Yilin,ZHANG Wenjie 2016, 12 (6):  331-334.  DOI: 10.3969/j.issn.1673-0364.2016.06.001 Abstract ( 312 )   Objective To explore the feasibility of using Au nanoclusters （AuNCs） to label the tissue engineering scaffold which degradation could be further monitored by noninvasive approach in vivo. Methods Chlorauric acid and bovine serum albumin （BSA） were used to synthesize AuNCs. The nanoclusters were characterized and their cytotoxicity was evaluated. AuNCs were then mixed with poly lactic-co-glycolic acid （PLGA） to fabricate AuNCs/PLGA scaffold. The scaffolds were implanted subcutaneously in nude mice. Fluorescence imaging and CT scanning were performed to detect the scaffolds in vivo. Results Synthesized AuNCs possess fluorescence without cytotoxicity. The AuNCs labeled PLGA scaffolds were detected by fluorescence imaging and CT scanning in vitro. The scaffolds were detected by these two noninvasive methods after being implanted in nude mice. Conclusion AuNCs could be potentially used for labeling polymer scaffold, which degradation could be possibly monitored by non-invasive imaging technology. Related Articles | Metrics

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A Study on the Repair of Bladder by Pro-Producing Bladder AceHular Matrix Graft-Silk Fibroin in Rats WANG Qiong,XIAO Dongdong,ZHAO Yang,ZHOU Zhe,ZHOU Juan,WANG Zhong,ZHANG Ming,LU Mujun 2016, 12 (6):  335-339.  DOI: 10.3969/j.issn.1673-0364.2016.06.002 Abstract ( 315 )   Objective To observe the role of subcutaneous pretreated bladder acellular matrix graft-silk fibroin （BAMG-SF） for bladder reconstruction in a rat bladder augmentation model. Methods After BAMG-SF was implanted subcutaneously for 1 day, 3 days, 7 days and 14 days in 4 four-week-old female Sprague Dawley rat, the scaffold and tissues around it were harvested for histology evaluation. Set 4 groups （n=3）： simple BAMG-SF （control group）, BAMG-SF 3 days subcutaneous precondition （3 days group）, BAMG-SF 7 days subcutaneous precondition （7 days group） and the autologous tissue around BAMG-SF 7 days subcutaneous precondition （autologous group）. All groups were used to reconstruct bladder in a rat bladder augmentation model, the bladder was harvested for histology and immunology evaluation 4 weeks later. Results After BAMG-SF implanted subcutaneously for 7 days, a large number of cells were observed in BAMG-SF. After BAMG-SF implanted subcutaneously for 14 days, BAMG-SF was covered by autologous tissue. The reconstruction of vascular and muscle in 3 days group were better than other groups, only the autologous group had no stone found. Conclusion BAMG- SF can be used for bladder repair, 3-day group has the best effect on bladder reconstruction and autologous group can avoid stone formation effectively. Related Articles | Metrics

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Research of Bone Marrow Mesenchymal Stem Cells Combined with Three-dimensional Printed Poly L-lactic Acid for Promoting Periosteal Distraction Osteogenesis of Rabbit Skull ZHAO Danyang,JIANG Wenbo,ZHANG Haifeng,DU Chao,DU Zijing,HAN Dong 2016, 12 (6):  340-352.  DOI: 10.3969/j.issn.1673-0364.2016.06.003 Abstract ( 469 )   Objective To investigate whether bone marrow mesenchymal stem cells （BMSCs） can promote periosteal distraction osteogenesis （PDO）. Methods BMSCs isolated from New Zealand white rabbits were cultured by the whole bone marrow adherent method and induced to differentiate into osteocytes, adipocytes and chondrocytes in vitro. Poly l-lactic acid （PLLA） scaffold produced by three-dimensional printing （3D printing） technology was used to distract the calvarial periosteum of rabbit. At the end of distraction period, BMSCs were injected into the gap between periosteum and skull in experimental group while the control group received equal normal saline （NS）. The distraction parts of rabbits＂ skull were collected after 4 and 8 weeks of consolidation periods. The newly formed bone of both groups was evaluated by Micro-CT scanning and histological staining. Results BMSCs obtained by the whole bone marrow culture method had the ability of multi-directional differentiation. Micro-CT scanning showed that there were new tissues formed in the distraction area at each time point both in experimental group and control group. The bone volume （BV）, bone volume/tissue volume （BV/TV）, bone mineral density （BMD）, trabecular number （Tb.N） and trabecular thickness （Tb.Th） in experimental group were higher than that in control group, however, the trabecular separation （Tb.Sp） in the experimental group was lower than that of the control group. HE staining also indicated new bone formation both in experimental group and control group. Compared with the control group, the newly formed trabecular bone in the experimental group was thicker and maturer, and the boundary between the new bone and the skull cortex was not obvious. Conclusion Injection of autologous BMSCs into the PDO region of rabbit skull can supplement the deficiency of osteocytes, which is beneficial to PDO. Related Articles | Metrics

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The Effects of Printing Technique in the Treatment of Cranlostenosis in Children LIU Tianjia,GU Shuo,WU Shuihua,FAN Shuangshi,CHEN Zhaohui 2016, 12 (6):  346-348.  DOI: 10.3969/j.issn.1673-0364.2016.06.004 Abstract ( 440 )   Objective To explore the effects of 3D printing technique in the treatment of Craniostenosis in Children. Methods The clinical data of 15 cases underwent the operation with the guidance of 3d printing between September 2015 and August 2016 were analyzed retrospectively. Children underwent Computed Tomography （CT） and 3D reconstruction of the shell before operations, the imaging data was transferred into 3d printing data through professional software, the deformity was reconstructed on the computer and the 1：1 skull model was printed by 3d printing technology, then the model was used to communicate with patients and make operative plans. The operations were finished as predetermined design, and the cranium CT scan was performed to observe the clinical efficacy. Results Fifteen cases underwent operations successfully as predetermined design, operative time ranged from 1.5 to 3.5 hours. According to postoperative cranial CT imaging and realistic appearances, the results were satisfactory. All the children were followed up for 2 to 12 months, no obvious comphcations were observed, and the progress of language and intellectual development was obvious. Conclusion Making use of 3d printing technology in the treatment of craniostenosis of children could save the operation time, reduce the surgical trauma, ensure the enough intro-cranial volume and postoperative appearances, promote the brain development obviously, and drive the trend of personalized therapy. Related Articles | Metrics

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An Improvement Model of Secondary Lymphedema in Hind Limb of Mouse SUN Yiyu,CUI Chunxiao,DAI Tingting,JIANG Chaohua,CAO Weigang,LI Shengli 2016, 12 (6):  349-352.  DOI: 10.3969/j.issn.1673-0364.2016.06.005 Abstract ( 549 )   Objective To establish a more stable and sustainable model of mouse lymphedema by adjusting the administrating timing and radiation doses. Methods Thirty-six female C57 mice aged 8weeks were randomly divided into 3 groups： ＂radiation before surgery＂ group （R＋S, n=12）, ＂radiation after surgery＂ group （S＋R, n=12）, and ＂radiation before and after surgery＂ group （r＋S＋r, n=12）. The experimental groups received radiation at varied time points： S＋R group, 4.5 Gy at 2 weeks after surgical treatment; R＋S group, 4.5 Gy at 3 days before surgery; and r＋S＋r group, 2.25 Gy at both 3 days before and 2 weeks after surgery. The volume change of the hind limbs of animals was measured by drainage method. Lymphangiogenesis in vivo situation was visualized by FDNG injection through the hind limb at 24th week after operation and immunohistochemistry staining was performed to show superficial lymphatic vessels. Results In six-month observation, all mice in the experimental groups formed lymphedema successfully. The r＋S＋r group showed the most stable hind limb lymphedema with lower mortality and morbidity rates. At 6 months post operation,according to the lymphatic mapping, the lymphatic vessels in R＋S and S＋R group increased, while rare lymphatic regeneration was observed in the r＋S＋r group. Immunohistochemistry revealed a decreased number of lymphatic vessels in the r＋S＋r group, compared with other groups （P〈0.05）. Conclusion A radiation dose of 2.25 Gy administered 3 days before surgery and 2 weeks after operation successfully enhanced the stability of the lymphedema model of mice hind limb. Related Articles | Metrics

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Research on Histological Changes and Expression of LIMK1 after Spinal Cord Injury in Rats LI Guangxian,WANG Riguang,JIANG Hongfeng,LIU Jinbang,NIU Yunfeng 2016, 12 (6):  353-363.  DOI: 10.3969/j.issn.1673-0364.2016.06.006 Abstract ( 324 )   Objective To observe the expression of LIMK1, GFAP and the histological changes after SCI, and to explore whether LIMK1 participate in the formation and control of pathological glial scar. Methods Fifty-four adult Wistar rats were randomly divided into 3 groups： one group was made as control group, one group only accepted the pseudo-operation, the others were made as spinal cord injury （SCI） model by the modified Allen＇s impact device. Locomotor capacity was assessed according to the 21-point Basso, Beanie and Bresnahan score; The lesions were observed with light microscopy and confocal laser scanning microscope at 1, 2, 3 and 4 weeks after SCI. The LIMK1 and GFAP were also observed by immunohistochemistry （IHC） and immunofluorescence assay. Results The mortality rate of SCI group was 33%. BBB scale of hind limb movements showed significant recovery of motor function after SCI in rats （P〈0.01）. There is a high degree of concordance between IHC and laser scanning confocal analysis for LIMK1 and GFAP. Both of the protein expression level increased at 1 week after SCI. The expression of GFAP reached the peak at 3 weeks after injury, and began to fail at 4 weeks. The peak of LIMK1 showed immediately at 1 week, then dropped at 2 weeks. But another peak was formed at 3 weeks and began to fail at 4 weeks. Conclusion After spinal cord injury, the expression of LIMK1 and GFAP are closely related. The expression profile of LIMK1 suggests that LIMK1 may play an important role in the glial scar development. Related Articles | Metrics

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Application of Digital Subtraction Angiography for Selecting Skin Flap in Hand Trauma XU Yekai,YUAN Siming,GUO Yao,CUI Lei,WANG Jun,HONG Zhijiang 2016, 12 (6):  357-359.  DOI: 10.3969/j.issn.1673-0364.2016.06.007 Abstract ( 324 )   Objective To explore the guiding effect of digital subtraction angiography （DSA） in selecting skin flaps for covering hand defects. Methods Retrospective analysis was made on six patients who had severe hand injury with the exposure of tendon and （or） bone. All the patients accepted DSA examination to determine the blood supply of forearm and hand, including radial artery, ulnar artery, perforating arteries, deep palmar arch and superficial palmar arch. According to the results of angiography, the vascular injury and the adjacent soft tissue conditions were evaluated. Then the reverse forearm island flap or distal free flap was selected to repair the wound. Results Four patients＇ radial artery, ulnar artery and palmar arehs were intact so that reverse forearm island flap was selected. The other two patients~ radial arteries were broken so that the free axial flap was selected. The flaps all survived completely. Conclusion DSA can clearly display the vasoganglion of the injured forearm and hand, detect the possible vascular injury, and effectively guide the selection of skin flap. The use of DSA improves the rationality of flap selection and the success rate of flap transplantation. Related Articles | Metrics

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Aesthetical Mini Wraparound Flap Supplied by Dorsal Digital Artery for Nail Bed Reconstruction GUO Shunu, LIU Mingbo, LU Youwen, LIU Liangyi, WANG Kelie, YANG Yanjun, ZHANG Ziqing 2016, 12 (6):  360-363.  DOI: 10.3969/j.issn.1673-0364.a464 Abstract ( 322 )   Objective To explore the surgical techniques and clinical effects of the mini wraparound flap supplied by the first dorsal digital artery for nail bed reconstruction. Methods From Jan 2012 to Jan 2016, the mini wrap-around flap supplied by the first dorsal digital artery was harvested to reconstruct nail bed defect, including 12 cases in thumb, 7 cases in the index finger, 2 cases in the middle finger and 2 cases in ring finger. The size of the defects ranged from （6 mm×12 mm）-（14 mm×28 mm）. The donor sites were directly closured, or covered with full-thickness skin grafts and pedicle flaps. Results All the flaps were survived. Patients were followed up for 6-48 months. Twenty-three cases were all satisfied with near-normal appearance and good function. No significant morbidity was observed at the donor site and the function was not affected. Conclusion Mini wraparound flap supplied by the first dorsal digital artery is an excellent method for entire or partial nail bed reconstruction. Related Articles | Metrics

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Correlation Analysis Between Infection and Hair Follicles after Expanded Polytetrafluoroethylene Implantation LI Dong,BAI Shanshan,YUAN Jie,WU Jieneng,HOU Rui,YU Zheyuan,XU Liang,WEI Min 2016, 12 (6):  364-367.  DOI: 10.3969/j.issn.1673-0364.2016.06.009 Abstract ( 328 )   Objective To investigate the correlation between infection and hair follicles after expanded polytetrafluoroethylene implantation, and to discuss the cause of infection. Methods Forty SD rats were randomly divided into the experimental group （n =20） and the control group （n =20）. In experimental group, e-FFFE was implanted subcutaneously （defined as group A） and subcorium （defined as group B） on the left and right side of the rats＂ back respectively. In control group, the left side （defined as group C） and the right side （defined as group D） of the rats＂ back were only separated anatomically. Samples were harvested at 1, 3 and 4 weeks post-operation. Samples were observed grossly and histologically. The corresponding inflammatory factors were detected via ELISA and statistical analysis was done. Results Swelling skins with effusion were found in group B, which got worse gradually. However, no similar symptom occurred in any other groups. Both histological and ELISA analysis indicated that group B undergone more severe inflammatory reaction than group A. Also the situation worsen throughout observation time. No inflammatory reaction was found in group C and D. The relevant differences were statistically significant. Conclusion E-PTFE contacted with hair follicles could lead to serious inflammation, and it is one of the important causes of local infection after rhinoplasty with e-PTFE. Related Articles | Metrics

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The Application of Ultrasonic Osteotome in Mandibular Angle Osteotomy HUANG Jinhua,YANG Xudong,ZHANG Qiang,MENG Zhibing,CHANG Caiwang,BIAN Jie,GUO Jun 2016, 12 (6):  368-370.  DOI: 10.3969/j.issn.1673-0364.2016.06.010 Abstract ( 419 )   Objective To investigate the effect of the ultrasonic osteotome in mandibular angle osteotomy. Methods Thirty-five cases who received simple mandibular angle osteotomy and regular reexamination were selected. Ultrasonic osteotome and bone knife handle were used cooperatively during the surgery. Results All 35 cases were followed up for 6 months to 5 years. Review of oral panorama film showed mandibular bilateral symmetry, smooth tnandibnlar margin, clear and complete mandibular nerve tube. Unilateral facial hematoma was observed in 1 patient. And the patient was recovered after 2 weeks of hematoma puncture and physical therapy. Conclusion Good results can be achieved in mandibular angle osteotomy by using ultrasonic osteotome. Related Articles | Metrics

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The Clinical Efficacy of Hyaluronic Filling in the Treatment of Facial Wrinkles DAI Qiang,SHI Wenjuan,CHEN Xiaoming,XU Qiliang 2016, 12 (6):  371-380.  DOI: 10.3969/j.issn.1673-0364.2016.06.011 Abstract ( 457 )   Objective To evaluate the clinical efficacy of hyaluronic filling in the treatment of facial wrinkles. Methods A total of 80 patients treated by hyaluronic filling for improving the facial wrinkles were enrolled in this study from January 2014 to June 2015. All patients were followed up for 6 months after the operation to analyze the improvement rate of facial wrinkles, adverse events and patients＇ satisfaction. Results For all 80 patients, the improvement rate of facial wrinkles was 93.8%, 90.0% and 86.3% at 1, 3 and 6 months after treatment. Mild adverse events were observed in the patients which was recovered after symptomatic treatment or automatically, and no serious adverse events were observed in these patients, including infection, tissue necrosis, granuloma, blood vessel embolism, etc. The overall satisfaction was 93.8%. Conclusion Hyaluronic filling can significantly improve the patient＇s facial wrinkles with long-lasting efficacy, low incidence of adverse reactions. Related Articles | Metrics

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The Application of Self-Made Protective Devices in Early Hand Protecting of Infant Post-Operation YANG Jiafei,WANG Hairong,BIAN Weiwei,LU Wei 2016, 12 (6):  373-374.  DOI: 10.3969/j.issn.1673-0364.2016.06.012 Abstract ( 303 )   Objective To evaluate the effect of self-made devices for early hand protecting in infants post-operation. Methods All 80 patients, from January 2015 to April 2016, were randomly divided into observation group （n=40） and control group （n=40）. Patients in control group were treated with routine nursing. Patients in observation group were treated with routine nursing in combination with self-made hand protective devices. All patients were evaluated one and two weeks post- operation in the following four aspects, including surgical site bleeding, the dressing slippage, Kirschner wires shift and infection. Results The results in observation group was better than in control group in all aspects one and two weeks post- operation. There were significant differences between the two groups （P〈0.05）. Conclusion Application of self-made protective devices can effectively improve hand protection of the infants after operation. It is helpful for the early rehabilitation in infants after operation. Related Articles | Metrics

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Role of MicroRNA in the Process of Osteogenic Differentiation of Mesenchymal Stem Cells CHEN Junbao,XIAO Ran,CAO Yilin 2016, 12 (6):  375-377.  DOI: 10.3969/j.issn.1673-0364.2016.06.013 Abstract ( 406 )   Mesenchymal stem cells have great application prospects in tissue engineering and regenerative medicine as seed cells, especially have evoked the extensive attention in the application of bone tissue engineering. Mechanisms of osteogenic differentiation of mesenchymal stem cells are complicated. It is affected by various factors. Different kinds of miRNA can inhibit or promote the osteogenic differentiation of mesenchymal stem cells respectively, it plays a key role in the differentiation process. In this paper, miRNA in different roles was classified and summarized, expecting to provide ideas for future research. Related Articles | Metrics

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Research Progress of Superparamagnetic Iron Oxide Nanoparticles on Labeling Stem Cells ZHANG Xintong,XIONG Meng 2016, 12 (6):  378-380.  DOI: 10.3969/j.issn.1673-0364.2016.06.014 Abstract ( 559 )   Superparamagnetic iron oxide nanoparticles （SPIONs） are a kind of nano materials which have broad application prospects in the field of labeling cells, targeted drug delivery and heat treatment of tumor. Especially in regenerative medicine, more and more research and application of stem cells have been founded. SPIONs have provided a new method to label and track cells. The characteristics of SPIONs are superparamagnetic, low toxicity, good biocompatibility and directional movement in the magnetic field. SPIONs present low signal area in magnetic resonance imaging （MRI） and they can label cells safely and effectively with appropriate coatings. But the uncertainty of cytotoxicity and the limitation of MRI are still unsolved. In this paper, the research progress of SPIONs in labeling cells were reviewed. Related Articles | Metrics

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Research Progress of Antibody Therapy in Melanoma ZHAO Hui,SUN Mengyan,WANG Yuchong,XUE Chunyu 2016, 12 (6):  381-384.  DOI: 10.3969/j.issn.1673-0364.2016.06.015 Abstract ( 441 )   Malignant melanoma is a kind of highly malignant tumor originated from neural crest melanin ceils, prone to distant metastases, the prognosis of patients with metastatic malignant melanoma is poor. Immunotherapy in recent years gradually becomes the new direction for treating malignant melanoma, a growing number of immune drugs are used in clinical treatment. There has been a revolution in the clinical management of melanoma with the development of multiple antibodies that influence T cell regulatory pathways, including checkpoint inhibition and co-stimulation. In this paper, two monoelonal antibodies （PD-1 and CTLA-4）, and two costimulatory pathways （OX40 and 4-1BB） were reviewed to describe the recent progress for treating melanoma. Related Articles | Metrics

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Research Progress of Laser Treatment in Congenital Melanocytic Nevus WANG Yongying,YANG Xi,CHEN Hui,LIN Xiaoxi 2016, 12 (6):  385-387.  DOI: 10.3969/j.issn.1673-0364.2016.06.016 Abstract ( 417 )   Congenital melanocytic nevus （CMN） is a kind of inborn pigmented nevus and occurs in approximately 1% of newborns. CMN may be cosmetically disfigured, also has possibility to malignant transformation. The most appropriate treatment for CMN is surgical excision. However, for large area of CMN, surgical excision would cosmetically cause scar in sensitive areas or in inoperable locations which is unsatisfactory. The lasers that have been studied for the treatment of CMN include pigment-specific lasers （ruby, alexandrite, Nd：YAG）, as well as ablative laser treatment with C02 laser and Er：YAG. In this paper, the progress of laser treatment in CMN was reviewed. Related Articles | Metrics

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Research Progress of Lipocyte Viability Assay BAE Jinhong,LI Qingfeng 2016, 12 (6):  388-390.  DOI: 10.3969/j.issn.1673-0364.2016.06.017 Abstract ( 457 )   Lipocyte viability assay is the standard for judgment of lipocyte＇s viability in vitro. It not only involves normal cell viability assay but also includes lipocyte-specific assessment. In this article, the principle, advantages, disadvantages and related applications of lipocyte viability assay were reviewed. Related Articles | Metrics