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Table of Content

    23 July 2020, Volume 9 Issue 4 Previous Issue    Next Issue
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    Original article
    Porcine Fetal Bone Marrow Mesenchymal Stem Cells in Cartilage Tissue Engineering
    LIU Lina,HE Aijuan,ZHOU Guangdong,CAO Weigang
    2013, 9 (4):  181-185.  DOI: 10.3969/j.issn.1673-0364.2013.04.001
    Abstract ( 371 )  
    Objective To investigate the optimal seed cell for cartilage engineering by comparing the chondrogenesis capability of porcine fetal bone marrow mesenchymal stem cells (fBMSCs) and adult porcine BMSCs (aBMSCs). Methods Fetal pigs with gestational age of 70 days were obtained by the uterine-incision delivery, and primary fBMSCs were isolated from the bone marrow. Primary aBMSCs were isolated from the bone marrow which was aspirated from iliac. Cell morphology of the two kinds of cells at passage 3 were observed after in vitro proliferation. The two kinds of cells at passages 3 were characterized by their osteogenic, adipogenic and chondrogenic differentiation potential. Then the fBMSCs and aBMSCs were separately seeded onto a polyglycolic acid/polylactic acid (PGA/PLA) scaffold with the concentration of 1 ×108 cells/mL. All specimens were harvested after 8 weeks' culture in vitro. Gross observation, glycosaminoglycan (GAG) quantification, total collagen quantification and histology were used to compare related characteristic differences of engineered cartilage formed by the two kinds of cells. Results fBMSCs had better proliferation and multiple differentiation capacity than aBMSCs. The two kinds of cells both formed mature cartilage after 8 weeks of culture in vitro, and the engineered cartilage of aBMSCs group had better appearance. The GAG content and total collagen content of the cartilage formed by fBMSCs were both higher than the cartilage formed by aBMSCs (P<0.01). Histology and immunohistochemistry demonstrated that the cartilage formed by fBMSCs have more compact tissue structure. The cartilage matrix staining of cartilage formed by fBMSCs were stronger than that of cartilage formed by aBMSCs. Conclusion The fBMSCs seems to be the optimal seed cells for cartilage tissue engineering.
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    The Efficacy of Different Stages of Induced BMSCs in Repairing Bone Injury of Rabbit
    ZHU Sha,LING Bin,SUN Jie
    2013, 9 (4):  186-189.  DOI: 10.3969/j.issn.1673-0364.2013.04.002
    Abstract ( 334 )  
    Objective To compare the efficacy of different stages of induced BMSCs in repairing bone injury of rabbit, and to find out the most suitable cells for transplantation. Methods BMSCs were isolated, cultured and identified in vitro. After labeled by GFP, BMSCs were induced and 40 rabbits were randomly divided into five groups according to different time of induction: non-induce group (n=5), 3-day group (n=10), 7-day group (n=10), 21-day group (n=10), control group (n=5). The bone injury model of rabbit was established. After successfully modeled for 24 hours, GFP labeled, induced BMSCs were injected into fracture area with the dosage of 3×106 cells/Kg. X-ray exam was carried out and evaluated. Results According to X-ray evaluation criteria, the X-ray score among non-induce group, 7-day group and 21-day group has no difference (P>0.05), but all significantly higher than control group (P<0.01). The X-ray score in 3-day group was higher than 7-day group and 21-day group (P<0.05), and significantly higher than control group (P<0.01). Conclusion BMSCs induced for 3 days turned out to be the most suitable cells for transplantation.
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    Proliferation Assay and Adipogenic Ability of Mouse Adipose-derived Stem Cells on Different Passages in Vitro
    JIANG Ting,YANG Zelong,BAI Qian,ZHOU Guangdong,LIU Wei
    2013, 9 (4):  190-194.  DOI: 10.3969/j.issn.1673-0364.2013.04.003
    Abstract ( 470 )  
    Objective To observe the proliferation capacity and adipogenic ability of mouse adipose-derived stem cells (mASCs) on different passages in vitro and to provide experimental evidence for rational selection of seed cells. Methods The mouse inguinal fat tissues were digested with collagenase, and cells were adhered to dishes, passage-cultured. The mASCs were divided into three groups: primary cells (P0), the second generation cells (P2) and the fifth generation cells (P5), and cell morphology and proliferation capacity of different passages of mASCs were observed. After four weeks of non-induction culture with high density seeding, the spontaneous adipogenic ability was observed through oil red staining and PT-PCR analysis. After two weeks of adipogenic induction, the adipogenic potential of mASCs was observed through oil red staining and PT-PCR analysis. Results The mouse adipose-derived stem cells displayed fibroblast-like morphology or irregular shape and different passages of them all had strong proliferation capacity. Primary cells had extremely strong spontaneous adipogenic ability in vitro. The spontaneous adipogenic ability of the second generation cells obviously reduced, and there was hardly any spontaneous adipogenic ability at passage 5. P0, P2 and P5 had similar induced adipogenic potential. Conclusion With the generation increasing, spontaneous adipogenic ability of mASCs reduced, but induced adipogenic potential almost unchanged. The fifth generation cells still maintain strong proliferation capacity and properties of stem cells. In a certain range of passage and amplification could reduce the content of pre-adipocyte and help purify the mesenchymal stem cells.
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    Umbilical Cord Mesenchymal Stem Cells in the Treatment of Hereditary Spastic Paraplegia
    LIU Jing,HAN Dongmei,WANG Zhidong,ZHENG Xiaoli,DING Li,ZHU Ling,XUE Mei,YAN Hongmin,GUO Zikuan,WANG Hengxiang
    2013, 9 (4):  195-203.  DOI: 10.3969/j.issn.1673-0364.2013.04.004
    Abstract ( 442 )  
    Objective To observe the safety and effectiveness of umbilical cord mesenchymal stem cells(UC-MSC) in the treatment of hereditary spastic paraplegia(HSP) from a familial father and son. Methods In September 2010 and April 2011, a familial father and son with HSP were given UC-MSCs by intrathecal injection for 2 courses, at a dose of 1x106/kg, once a week, 4 times as a course. Modified Ashworth Scale(MAS), International Cooperative Ataxia Rating Scale(ICARS) and Activity of Daily Living Scale (ADL) were used to evaluate patients' neural function and quality of daily life before and after cell therapy. Results Comparing their MAS grading, ICARS and ADL scores one month after the first course therapy with those before therapy, it is found that MAS grading, ICARS and ADL scores were decreased, their stability of walking and standing, as well as verbal fluency were improved. Comparing their ICARS and ADL scores one month after the second course therapy with those before the second course therapy, it is found that their ICARS and ADL scores were decreased, son ’s muscular tension was further reduced, and father’s ataxia was relieved. No side effect was observed in both of them. A follow-up of 20 months after the second course therapy showed that the father and son ’s symptoms progressed respectively at the time of 7 months and 8 months after all therapy. Conclusion Intrathecal injection of UC-MSCs is safe and can ameliorate clinical symptoms, improve life quality of HSP patients to some extent within a certain period, and slow down disease progression, but efficacy can not permanently maintain.
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    Application of Resina Draconis Extract on Accelerating Animal Wound Healing
    LIU Huihui,XIAO Dan,ZHENG Xiao,GU Yan,GUO Shanyu
    2013, 9 (4):  199-203.  DOI: 10.3969/j.issn.1673-0364.2013.04.005
    Abstract ( 431 )  
    Objective To investigate the effects of ethanolic extract of Resina Draconis (RDEE) in animal wound healing. Methods Forty-eight SD rats were randomly divided into three groups: control group, MEBO group (treated with MEBO) and RDEE group (treated with RDEE). Wound healing rates and healing time were calculated 3, 7, 11 and 15 days after treatment, and tissues were harvested at the same time for histological, immunohistochemical analysis and MVD calculation. The expression of VEGF was determined by real-time PCR and western blot. Results Wound healing time in RDEE group was shorter than in control group (P<0.05). There was no difference of would healing time between RDEE group and MEBO group. Wound healing rates, MVD number (3, 7, 11 days after treatment) and the expression of VEGF were significantly higher in RDEE group and MEBO group than in control group (P<0.05). Histological results showed more well-organized bands of collagen, more fibrob-lasts and less inflammatory cells in RDEE group compared with control group. Conclusion The extract from Resina Draconis possesses wound healing activity, and is worthy of clinical application.
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    Pre-operation 3D Scanning and Simulation in Nasal Reconstruction
    JIANG Cheng’an,LI Qingfeng,LIU Kai
    2013, 9 (4):  204-207.  DOI: 10.3969/j.issn.1673-0364.2013.04.006
    Abstract ( 374 )  
    Objective To provide useful reference for the pre-operative design and post-operative estimation by pre-operation 3D scanning and simulation in nasal reconstruction. Methods Facial scans of normal crowd and patients with nasal defect were acquired, then nasal region fitting patients' facial contour was simulated. Simulated nose were measured by computer to provide valuable information for nasal reconstruction. The data of simulation and postoperative result were compared and analyzed. Results 3D scanning and simulating technique proved to be helpful in providing useful data such as flap area and dimensions of anatomical parts for nasal reconstruction surgery. Statistical analysis showed no difference between the data of simulation and postoperative result. Conclusion 3D scanning and simulation could assist the pre-operative and post-operative estimation for nasal reconstruction, and play a great role in improving operative effect and patients’ satisfaction.
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    Measurement and the Significance on the Activity of Nasal Tip of the Youth Female in Han Nationality
    HOU Rui,WEI Min,Yuan Jie,YU Zheyuan
    2013, 9 (4):  208-209.  DOI: 10.3969/j.issn.1673-0364.2013.04.007
    Abstract ( 388 )  
    Objective To investigate the activity of nasal tip of the youth female in Han nationality comparing with the activity of nasal tip of those who have undergone rhinoplasty, and to provide morphologic basis and assessment criteria for nasal plasty. Methods External noses of 100 youth female (natural group) and 30 female undergoing rhinoplasty with alloplastic materials (operative group) were chosen. After the activity critical point A was confirmed, the activity of nose tip, the length of A-P and the nasal length were measured. Results The activity of nasal tip in natural group was 35.73 ° ± 4.72 ° , and 21.45 ° ± 7.53 ° in operative group. There were significant differences between 2 groups. The length of A-P in natural group was (16.62 ±1.95) mm while it was (25.89±10.16) mm in operative group. The nasal lengths were both in normal range. In operative group, 63%of the patients felt the decent of the nasal tip activity, 30% were not satisfied with the operation because of this. Conclusion Rhinoplasty with alloplastic materials could lower the activity of nose and have influence on the patients ’ assessment for the surgery.
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    Expended Muscle-Sparing Latissimus Dorsi Myocutaneous Flap for the Repair of Extensive Scar Contracture Deformity in Chest
    TIAN Baoxiang,LIN Hailong,ZAN Tao,FAN Hua,LIU Fengbin,LI Qingfeng,GU Bin
    2013, 9 (4):  210-212.  DOI: 10.3969/j.issn.1673-0364.2013.04.008
    Abstract ( 415 )  
    Objective To explore the clinical application of expanded muscle-sparing latissimus dorsi myocutaneous flap in repairing extensive scar contracture deformity of chest. Methods From October 2010 to October 2012, 8 cases with extensive scar contracture deformity in chest were treated by expended muscle-sparing latissimus dorsi flap, including 3 bilateral cases. The operation was divided into two stages. Stage one: A Doppler probe was used to mark the location of lateral myocutaneous perforators of thoracodorsal artery (LMTA). The muscle-sparing latissimus dorsi flap was designed based on LMTA. Flap undermined in accordance with design, and then tissue expander was implanted. The expansion time was 4 to 6 months. Stage two: The scars were excited, the breast tissue was relocated at the normal anatomic position. The expended muscle-sparing latissimus dorsi flap was transplanted to cover the defects. Results All the flaps survived, and all donor sites were closed primarily. All the patients were followed up for 3 to 20 months, the texture and color of the flap was similar to chest skin. There was no difference in strength or range of motion around shoulder joint comparing with opposite shoulder joint. No depressed deformity was observed and the appearance of breast was excellent in female patients. Conclusion It is an ideal method to repair extensive scar contracture deformity in chest with expended muscle-sparing latissimus dorsi myocutaneous flap.
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    Combined Skin Flaps for the Repair of Severe Tissue Defects of Ankle and Foot
    QIAN Dejian,GUO Xiangkai,LIU Yunan,LIU Wei,DOU Lianda,ZHAO Gang
    2013, 9 (4):  213-215.  DOI: 10.3969/j.issn.1673-0364.2013.04.009
    Abstract ( 365 )  
    Objective To explore the efficacy of combined skin flaps for the repair of severe tissue defects of ankle and foot. Methods From Oct. 2000 to May. 2012, 13 patients with severe ankle and foot trauma were repaired by sural neurovascular flap and cross-leg flap. Results Twelve flaps were survived and one case suffered from necrosis. Survived flaps were healed by first intension. All the patients were followed up for 6 to 12 months, good functional recovery was observed. Conclusion Combined skin flap is a good way to repair severe tissue defects of ankle and foot with its safety, simplicity and good functional restoration.
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    The Modified Temporalis Muscle Flap for Correcting Depressed Deformity of Donor Site after Surgery
    LI Pingdong,CHEN Xiaohong
    2013, 9 (4):  216-217.  DOI: 10.3969/j.issn.1673-0364.2013.04.010
    Abstract ( 353 )  
    Objective To evaluate the modified temporalis muscle flap (TMF) for correcting depressed deformity of donor site after surgery. Methods In this retrospective study, 15 patients with malignant maxillary tumor were involved. The modified temporalis muscle flap was used to repair the defect after tumor resection. Results All the patients were followed up for 12-54 months. All flaps were survived, except one case suffered from operative incision split and healed itself 3 months after radiotherapy. Epithelization of the fascia in oral cavity was completed in 4-6 weeks. Good functional reconstruction was achieved and no temporal deformity was observed. The cosmetic results were better compared with traditional temporalis muscle flap. Conclusion The modified TMF, the same as traditional temporalis muscle flap, can be served as the first-line reconstructive option for maxillary defects. And it can get better temporal cosmetic results with more satisfaction.
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    Treatment of Chronic Mallet Finger Deformity by Micro Arc Bone Anchor Combining Palmaris Longus Tendon Graft
    YAO Junna,DU Zhijun,ZHAO Zuogong,LuoYang Orthopedic Traumatological Hospital
    2013, 9 (4):  218-240.  DOI: 10.3969/j.issn.1673-0364.2013.04.011
    Abstract ( 477 )  
    Objective To evaluate the clinical outcomes of treating chronic mallet finger deformity by micro arc bone anchor combined with palmaris longus tendon graft. Methods All16 cases suffering from chronic mallet finger deformity were treated by micro arc bone anchors combining with palmaris longus tendon graft to rebuild the insertions of extensor tendon. Functional exercises, regular reviews and functional assessments were started 6 weeks after surgery. Results All the patients were followed up for 4-10 months, no operational infection or foreign body reaction was observed. The X-ray films showed no loose or fell off of micro-anchors. The distal inter-phalangeal joints were stable with excellent in 12 cases, good in 2 cases, passable in 1 case and unsatisfactory in 1 case according to Dargan's method. Conclusion The treatment of chronic mallet finger deformity by micro arc bone anchor combining palmaris longus tendon graft is simple in process and certain in effects. It is a better method to rebuild the insertions of extensor tendon and is worthy to popularize.
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    Clinical Effect of Percutaneous Kyphoplasty in Treatment of Osteoporotic Vertebral Compressive Fracture
    QU Yi,YU Xing,BI Lianyong,WANG Fengxian,CAO Xu,YANG Jizhou,JIAO Yong
    2013, 9 (4):  220-222.  DOI: 10.3969/j.issn.1673-0364.2013.04.012
    Abstract ( 265 )  
    Objective To explore the clinical effect of percutaneous kyphoplasty in treatment of osteoporotic vertebral compressive fracture. Methods From April 2010 to November 2012, 80 patients with osteoporotic vertebral compressive fracture were randomly divided into 2 groups, experimental group (n=40) and control group (n=40). Patients in experimental group were treated with percutaneous kyphoplasty, while patients in control group were treated with percutaneous vertebro-plasty. Results All the patients were followed up for 6 months. The VAS score, Cobb's angle, and ODI score after operation were significantly lower than those before operation in both two groups (P<0.05). The VAS score in experimental group was significantly lower than that in control group 6 months after operation (P<0.05). Furthermore, the Cobb's angle and ODI score in experimental group were significantly lower than those in control group 3 days and 6 months after operation ( P<0.05). In addition, the leakage rate of bone cement in experimental group was significantly lower than that in control group after operation (P<0.05). Conclusion Percutaneous kyphoplasty in treatment of osteoporotic vertebral compressive fracture is effective and reliable. It can be the preferred method in treatment of osteoporotic vertebral compressive fracture.
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    The Effect of Widening Palpebral Fissure by Double-folded Blepharoplasty Combining with Epicanthoplasty and Lateral Canthoplasty
    SUN Qingqing,LIU Kai
    2013, 9 (4):  223-224.  DOI: 10.3969/j.issn.1673-0364.2013.04.013
    Abstract ( 357 )  
    Objective To observe the effect of widening palpebral fissure by double-folded blepharoplasty combining with epicanthoplasty and lateral canthoplasty. Methods Epicanthus was corrected by modified method of transection and longitudinal suture, and lateral canthoplasty was carried out by upper palpebral flap crossing lower eyelid conjunctiva flap. Double-folded blepharoplasty used the method of incision and the incisions of double eyelid, inner and outer canthus were not connected. Results In 20 patients, epicanthus was disappeared, inner canthus and out canthus were respectively 5-8 mm and 2-3 mm wider after operation, and palpebral fissure obviously opened. The follow-up period ranged from 1 to 2 years. The effect of widening palpebral fissure kept stable with inconspicuous scar in inner and outer canthus. Conclusion Double-folded blepharoplasty combining with epicanthoplasty and lateral canthoplasty can significantly increase the length and width of palpebral fissure with inconspicuous scar in inner and outer canthus, and is an effective method to widen the palpebral fissure.
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    Research Progress of Epidermal Stem Cells
    ZHOU Shuping,ZONG Xianlei,NIU Fuyou,LIU Lingbo,CAI Jinglong
    2013, 9 (4):  225-228.  DOI: 10.3969/j.issn.1673-0364.2013.04.014
    Abstract ( 452 )  
    Epidermal stem cells (ESCs) located in cuticles layer, have the ability of constantly proliferation and differentiation. In recent years, many researches were carried out and made some achievements on ESCs, bringing a new method for clinical application. The research progress on the location, the markers, the characteristics, the regular mechanism and application of ESCs were reviewed in this paper.
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    Surface Marker Molecules of Human Mesenchymal Stem Cell
    LI Qiuchen,XIAO Ran
    2013, 9 (4):  229-234.  DOI: 10.3969/j.issn.1673-0364.2013.04.015
    Abstract ( 538 )  
    The mesenchymal stem cells (MSCs) are the ideal seed cells for tissue and organ repair. However, different isolating methods can lead to different MSCs purity which greatly affect the therapeutic effect. Therefore, using surface markers to identify and sort MSCs has become a hotspot in the field of stem cell research. This review summarized the classification, function, application and the stability of human MSC surface markers.
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    Research Progress of Vascularization in Tissue Engineering
    WANG Yuchong,XUE Chunyu
    2013, 9 (4):  232-234.  DOI: 10.3969/j.issn.1673-0364.2013.04.016
    Abstract ( 648 )  
    Nowadays the main problem in tissue engineering both in vitro and in vivo is the lack of sufficient blood supply - the vascularization. There are two main current strategies to create vascularized tissues. The first strategy is based on the cells and their ability to form new vessels. In this process growth factors as well as cells are contribute to generate new vessels. The second strategy is scaffold based techniques, including naturally -derived scaffolds and synthetically manufactured scaffolds. Future strategies to create vascularized tissues were reviewed.
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    Treatment of Metaphyseal Tibia Fractures by Intramedullary Nailing
    XIONG Chao,WANG Yongqing,JIANG Wenxue
    2013, 9 (4):  235-240.  DOI: 10.3969/j.issn.1673-0364.2013.04.017
    Abstract ( 301 )  
    Tibial fractures are the most common long bone fractures. The benefits of intramedullary nailing of these fractures include load sharing, sparing of the extraosseous blood supply and avoidance of additional soft-tissue dissection. But intramedullary nailing also causes different complications, like primary and secondary mal-alignment, malunion, delayed healing and non-healing of tibial metaphyseal fractures. Several nailing methods and reduction techniques have been developed to minimize these complications. The improvements of nailing methods and reduction techniques of intramedullary nailing were reviewed.
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