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23 July 2020, Volume 8 Issue 1 Previous Issue    Next Issue

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A Preliminary Study:Chondrogenesis of CD204(-) Human Adipose-Derived Stem Cells In vitro DING jinping,SUN hengyun,CHEN jinjun,ZHANG Wenjie,LIU Wei,CAO Yilin,ZHOU Guangdong 2012, 8 (1):  1-5.  DOI: 10.3969/j.issn.1673-0364.2012.01.001 Abstract ( 361 )   Objective To compare the characteristics and chondrogenesis between CD204(-) ADSCs and CD204(+) ADSCs in vitro.Methods ADSCs were isolated and cultured,then the CD204(+) ADSCs and CD204(-) ADSCs were sorted by flow cell sorting.After expansion to passage 2,the morphology of CD204(+) and CD204(-) cells were observed in vitro.Adipogenic and osteogenic lineage differentiation in two cell groups were assessed by Oil Red O and alizarin red staining.Meanwhile,the two cell groups were cultured in chondrogenic media forming cell pellet and assessed by HE,Safranin O and collagen II staining.Results The expression of CD204 in adherent cells reached about 10% by FACS.Both of them all demonstrated fibroblast-like morphology and CD204(-) cells showed better proliferation ability.After adipogenic induction for 10 days,large amount of lipid-filled droplets were observed in two cell groups by Oil Red O staining,but CD204(+) cells demonstrated a better adipogenic ability.After osteogenic induction for 2 weeks,stronger positive staining was observed in CD204(-) cell group by alizarin red staining.After chondrogenic induction,the pellets formed by CD204(-) cells had a transparent white cartilage-like morphology while the CD204(+) cell pellets had a yellowish appreance.Histologically,the CD204(-) group showed a homogeneous cartilage-like structure with typical lacuna,strong safranin-O staining and strong collagen II staining,but pellets of CD204(+) group were almost fibrosis.Conclusion CD204(-) cells have the same characteristics with stem cells and high chondrogenic potential.CD204(-) cells could be an ideal seed cells in cartilage tissue engineering. Related Articles | Metrics

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The Optimal Cycle of Detergent-enzymatic Method for Rabbit Tissue Engineering Tracheal Matrix LI Jianzhong,ZHANG Zhipei,WANG Jian,WU Fan,JIN Yan,HAN Yong,JIANG Tao,YAN Xiaolong,NI Yunfeng,ZHAO Jinbo,LI Xiaofei 2012, 8 (1):  6-31.  DOI: 10.3969/j.issn.1673-0364.2012.01.002 Abstract ( 367 )   Objective To explore the optimal cycle of detergent-enzymatic method for rabbit tissue engineering tracheal matrix.Methods Twenty-four healthy New Zealand rabbits were randomly divided into 4 groups(n=6),the tracheas of group A was treated with nothing as control group,the tracheas of group B,C and D were treated with two,three and four cycles of the detergent-enzymatic method separately.The histologic appearance,ultrastructure,maximum drawing force,rupture force and mutation rate were observed.Results The maximum drawing force,rupture force and mutation rate on group B and group C were bigger than group A and group D.There were many epithelial cells in the tracheas of group A,some epithelial cells in the tracheas of group B,but none epithelial cells in the tracheas of group C and group D by histological observation.The extracellular matrix and collagen fiber in group A,B,C were significantly more than in group D by SEM.Conclusion The tracheals treated with three cycles are more nonimmunogenic,have better biomechanical performance and more ECM.It is suitable for reconstruction of allograft engineering tracheal. Related Articles | Metrics

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Necrosis of The Lung Cancer Cell line A549 Treated with Hydroxyapatite Nanoparticles Carried Cisplatin In Vitro TAO Xian-dong,ZHONG Lei,XUE Lei,ZHAO Xue-wei 2012, 8 (1):  14-17.  DOI: 10.3969/j.issn.1673-0364.2012.01.004 Abstract ( 272 )   Related Articles | Metrics

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The Influence of the Thickness of PDLLA Absorbable Membrane on Adhesion and Healing of Tendon: A Preliminary Study Guo Xiaopeng,Wang Xiaopan,Shen Zunli,Huang Yixiong,Zhang Zhaofeng 2012, 8 (1):  18-22.  DOI: 10.3969/j.issn.1673-0364.2012.01.005 Abstract ( 399 )   Related Articles | Metrics

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Comparison of Measurement in the Ocular Region between the Direct Measurement and Photogrammetry TANG Mengyao,CHENG Liying,ZHANG Yuguang 2012, 8 (1):  23-25.  DOI: 10.3969/j.issn.1673-0364.2012.01.006 Abstract ( 393 )   Related Articles | Metrics

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Bone Allograft Embedded by Vascularized Periosteum Flap for the Reconstruction of Segmental Bone Defect ZHANG Dawei,TIAN Qingye,LIU Guangjun,LIU Xuetao,WANG Qian,YANG Lei 2012, 8 (1):  26-31.  DOI: 10.3969/j.issn.1673-0364.2012.01.007 Abstract ( 423 )   Objective To explore the effect of bone allograft enbedded by vascularized thin cortex-periosteum flap for treatment of segmental bone defect.Methods The adult rabbit model of segmental tibial defect was established.The allograft de-antigenic rabbit tibia was designed and opened a window.Then the window was embedded by vascularized cortex-periosteum flap as a complex.Sixty healthy rabbits were randomly divided into 3 groups: experimental group,control group and blank group.In experimental group,the defect was repaired by complex,vascularized flap packing the allografts was used as the control group and only allografts was used as the blank group.X-ray examination,gross observation,histological observation and immunohistochemical examination were done on the bone and the surrounding tissues.Results In the experimental group,callus formation,transformation remodeling and neovascularization were observed.The vascularization,new bone formation and bone units mature were earlier in the experimental group than the control group.Conclusion Bone allograft enbedded by vascularized thin cortex-periosteum flap could reduce the time of bone repairment and has significant advantages compared to the directly packing model. Related Articles | Metrics

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Free Tensor Fasciae Latae Perforator Flap in Repairing Soft Tissue Defect of Hands and Feet ZHANG Gonglin,CHEN Keming,Yu Hui,WANG Gansheng,ZHAO Laixu,YANG Junlin 2012, 8 (1):  32-34.  DOI: 10.3969/j.issn.1673-0364.2012.01.008 Abstract ( 337 )   Related Articles | Metrics

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Application of Two Directions Folding of Platysma Flap in Reconstruction for Contracture Deformity of Severe Cervical Scar JI Geng,WANG Shoubao,LUO Xusong,LI Luozhu,YANG Jun 2012, 8 (1):  35-39.  DOI: 10.3969/j.issn.1673-0364.2012.01.009 Abstract ( 381 )   Objective To introduce a surgical technique of two directions folding of platysma flap in reconstruction for cervical cicatrix contracture.Methods Fifteen patients with cervical scar contracture were treated with split to full thickness skin grafts,combined with platysma flap double directions folding that deepen cervico-mandibular angle and cover scar section.Results All skin grafts survived and natural cervical appearance with smooth transition and improved activity were achieved.Conclusion The new technique of two directions folding of platysma flap was proved to be effective in the treatment of cervical scar contracture. Related Articles | Metrics

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The Modified Vacuum Sealing Drainage (VSD) Lavage in Treating Refractory Wound HAN Fei,CHEN Zhengyu,LI Xin,LIU Huaxing 2012, 8 (1):  40-45.  DOI: 10.3969/j.issn.1673-0364.2012.01.011 Abstract ( 364 )   Related Articles | Metrics

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Evaluation of Absorbable Mini Anchors for Reconstruction of Extensor Tendon Terminal Rupture LU Jian-feng,LIU Zong-bao,CUI Zhi-hao 2012, 8 (1):  43-45.  DOI: 10.3969/j.issn.1673-0364.2012.01.012 Abstract ( 513 )   Related Articles | Metrics

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Advancement in Hepatocyte Immortalizaiton Ma Di 2012, 8 (1):  46-48.  DOI: 10.3969/j.issn.1673-0364.2012.01.013 Abstract ( 416 )   Related Articles | Metrics

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The Immunological Characteristics and Allogeneic Application of Bone Mesenchymal Stem Cells WU Jingguo,CAO Yilin,XIAO Ran 2012, 8 (1):  49-52.  DOI: 10.3969/j.issn.1673-0364.2012.01.014 Abstract ( 453 )   Bone mesenchymal stem cells(BMSCs) have become a research hotspot in regenerative medicine and immunology,owing to its multi-directional differentiation potential,low immunogenicity and immunomodulatory during allografting.Allogeneic BMSCs(allo-BMSCs) have been shown a positive role in regenerative medicine,tissue engineering and immunoregulation,but there are still some problems to be solved before its clinical application.The Immunological Characteristics and Allogeneic Application of Bone Mesenchymal Stem Cells were reviewed. Related Articles | Metrics

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Progress of Adipose-derived Stem Cells in Bone Tissue Engineering GU Zhen 2012, 8 (1):  53-55.  DOI: 10.3969/j.issn.1673-0364.2012.01.015 Abstract ( 325 )   Related Articles | Metrics

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The Regulation of Differentiation on Oligodendrocyte Precursor Cells JIANG Meng 2012, 8 (1):  56-59.  DOI: 10.3969/j.issn.1673-0364.2012.01.016 Abstract ( 426 )   Related Articles | Metrics