低体重早产儿内皮祖细胞体外血管生成能力研究

doi:10.16150/j.1671-2870.2018.03.017

诊断学理论与实践 ›› 2018, Vol. 17 ›› Issue (03): 318-321.doi: 10.16150/j.1671-2870.2018.03.017

低体重早产儿内皮祖细胞体外血管生成能力研究

丁效薇^a, 张如愿^b, 李然然^b, 唐海婷^a

上海交通大学医学院附属瑞金医院a. 妇产科; b. 重症医学科,上海 200025

收稿日期:2017-11-16 出版日期:2018-06-25 发布日期:2018-06-25
通讯作者: 唐海婷 E-mail: 281682208@qq.com
基金资助:
国家自然科学基金（81301614）

Study on angiogenic properties of endothelial progenitor cells in low birth weight preterm infants

DING Xiaowei^a, ZHANG Ruyuan^b, LI Ranran^b, TANG Haiting^a

a. Department of Obstetrics and Gynaecology, b. Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received:2017-11-16 Online:2018-06-25 Published:2018-06-25

摘要/Abstract

摘要： 目的：探讨低体重早产儿的内皮祖细胞（endothelial progenitor cells, EPCs）血管生成能力及调控机制。方法：收集15例低体重早产儿（胎龄小于37周且体重小于 2.5 kg）和19例对照足月儿（胎龄大于37周且体重大于等于2.5 kg）的脐带血,于诱导分化前用流式细胞仪检测EPCs（CD34⁺/CD133⁺/VEGFR2⁺）的数量。分离培养后,检测EPCs克隆形成时间、克隆形成能力、细胞增殖能力和小管形成能力。用蛋白印迹法检测EPCs中血管内皮钙粘蛋白（vascular endothelial cadherin,VE-cadherin）和血管内皮生长因子受体2（vascular endothelial growth factor receptors 2,VEGFR2）的表达情况。结果：与足月儿相比（0.003%±0.002%）,低体重早产儿脐带血中的EPCs百分比（0.0026%±0.0025%）并无变化,但EPCs克隆形成时间推迟（18 d比12 d）,克隆形成能力、细胞增殖能力及小管形成能力均较足月儿下降（P均<0.05）。蛋白印迹法检测结果显示,低体重早产儿脐带血EPCs中的VE-cadherin和VEGFR2表达降低。结论：低体重早产儿的EPCs血管生成能力受损,可能与EPCs中血管内皮特异性标志物VE-cadherin、VEGFR2表达降低有关。

关键词: 内皮祖细胞, 脐带血, 新生儿

Abstract: Objective: To investigate the angiogenic properties of cord blood endothelial progenitor cells (EPCs) in low birth weight(LBW) preterm infants. Methods: Venous umbilical cord blood was collected from 19 cases of full-term neonates with normal weight and 15 LBW neonates. The relative number of cord blood EPCs (CD34⁺/CD133⁺/VEGFR2⁺) from LBW preterm and full-term neonates was counted by ?ow cytometry. The angiogenic properties of cord blood EPCs were evaluated for the time for clone formation, clone forming ability and capacity to proliferate and formtubes in vitro. Results: There was no difference in the relative number of cord blood EPCs between LBW preterm and full-term neonates. Compared with EPCs of full-term neonates, the time for clone formation of EPCs was delayed in LBW, while clone forming ability and capacity to proliferate and form tubes were decreased. The expressions of VEGFR2 and VE-cadherin of EPCs in LBW were significantly reduced. Conclusions: Angiogenic properties of EPCs are impaired in LBW preterm infants, and this may be due to the decreased expression of VE-cadherin and VEGFR2.

Key words: Endothelial progenitor cells, Umbilical cord blood, Neonates

中图分类号:

R722.1

丁效薇, 张如愿, 李然然, 唐海婷. 低体重早产儿内皮祖细胞体外血管生成能力研究[J]. 诊断学理论与实践, 2018, 17(03): 318-321.

DING Xiaowei, ZHANG Ruyuan, LI Ranran, TANG Haiting. Study on angiogenic properties of endothelial progenitor cells in low birth weight preterm infants[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(03): 318-321.

参考文献

[1] Irving RJ, Belton NR, Elton RA, et al.Adult cardiovascular risk factors in premature babies[J]. Lancet,2000,355(9221):2135-2136.
[2] Siewert-Delle A, Ljungman S.The impact of birth weight and gestational age on blood pressure in adult life: a population-based study of 49-year-old men[J]. Am J Hypertens,1998,11(8 Pt 1):946-953.
[3] Bonamy AK, Bendito A, Martin H, et al.Preterm birth contributes to increased vascular resistance and higher blood pressure in adolescent girls[J]. Pediatr Res,2005, 58(5):845-849.
[4] Asahara T, Murohara T, Sullivan A, et al.Isolation of putative progenitor endothelial cells for angiogenesis[J]. Science,1997,275(5302):964-967.
[5] Tauzin L, Rossi P, Giusano B, et al.Characteristics of arterial stiffness in very low birth weight premature infants[J]. Pediatr Res,2006,60(5):592-596.
[6] Ligi I, Simoncini S, Tellier E, et al.A switch toward angiostatic gene expression impairs the angiogenic properties of endothelial progenitor cells in low birth weight preterm infants[J]. Blood,2011,118(6):1699-1709.
[7] Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors[J]. Nat Med,2003,9(6):669-676.
[8] Ligi I, Simoncini S, Tellier E, et al.Altered angiogenesis in low birth weight individuals: a role for anti-angiogenic circulating factors[J]. J Matern Fetal Neonatal Med,2014, 27(3):233-238.
[9] Carmeliet P, Lampugnani MG, Moons L, et al.Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis[J]. Cell,1999,98(2):147-157.
[10] Calera MR, Venkatakrishnan A, Kazlauskas A.VE-cadherin increases the half-life of VEGF receptor 2[J]. Exp Cell Res,2004,300(1):248-256.

[1]	杜燕然, 焦景, 任芸芸, 周建桥. 超声影像组学技术在评估胎肺成熟度中的应用[J]. 诊断学理论与实践, 2022, 21(03): 326-330.
[2]	沈静, 叶珍, 王淑平, 陈慧芬. 上海地区14 770名孕妇在不同孕周进行不规则抗体筛查的意义及安全策略探讨[J]. 诊断学理论与实践, 2021, 20(01): 71-76.
[3]	孙文瑾, 王淑平, 汤伟娴, 陈慧芬. 7 381例孕妇产前不规则抗体检测特异性分析及临床意义[J]. 诊断学理论与实践, 2019, 18(03): 365-367.
[4]	汤伟娴, 金沙, 郑皆炜, 叶珍, 刘曦, 沈伟. 孕妇IgG抗A（抗B）的效价积分对新生儿溶血病贫血严重程度的预判价值[J]. 诊断学理论与实践, 2018, 17(04): 449-452.
[5]	陆世娟, 尚庆毅, 蒋志红, 陈同排, 张小庆. IP-10联合PCT和hs-CRP检测对新生儿感染性疾病的诊断价值[J]. 诊断学理论与实践, 2018, 17(03): 304-307.
[6]	沈晓红, 陈慧芬, 张军, 叶剑波, 张贤华. 2014年至2017年上海地区新生儿血流感染病原菌分布及其耐药性分析[J]. 诊断学理论与实践, 2018, 17(03): 266-271.
[7]	周剑辉, 周文英, 徐倩, 冯健明, 王毅. 葡萄糖-6-磷酸脱氢酶测定试剂盒（荧光比值法）的研制[J]. 诊断学理论与实践, 2016, 15(06): 623-626.
[8]	叶宝英, 陈炯, 李丽蟾, 陈瑞玉, 施立晔, 牛建梅,. 正常早期新生儿组织多普勒成像心肌活动指数分析[J]. 诊断学理论与实践, 2016, 15(03): 240-243.
[9]	陆子贇, 彭怡倩, 应春妹,. 降钙素原和C反应蛋白联合检测在新生儿感染性疾病诊断中的应用评估[J]. 诊断学理论与实践, 2016, 15(02): 157-159.
[10]	蔡晓红, 王学锋,. 胎儿新生儿溶血病的实验诊断[J]. 诊断学理论与实践, 2015, 14(06): 507-510.
[11]	Kaustuv Bhattacharya, Tiffany Wotton, Veronica Wiley, 侯宇航, 吴占河,. 血片技术筛查新生儿疾病的过去、现在和将来[J]. 诊断学理论与实践, 2015, 14(02): 109-112.
[12]	陈飞,. 微柱凝胶法动态检测O型孕妇ABO血型IgG抗体效价的临床意义[J]. 诊断学理论与实践, 2015, 14(01): 62-63.
[13]	梁肖云, 曾兰兰, 李文利, 梁绮华,. 网织红细胞参数在新生儿黄疸诊断中的价值[J]. 诊断学理论与实践, 2011, 10(04): 350-352.
[14]	尹俊, 石刚刚, 李春海, 姚关锋, 宋卉, 樊化平, 高分飞, 郑庆棠,. 人脐带血干细胞移植防治兔急性心肌缺血的实验研究[J]. 诊断学理论与实践, 2006, 5(06): 511-514.
[15]	朱铭, 钟玉敏, 张弘,. 新生儿心脏病的磁共振检查[J]. 诊断学理论与实践, 2006, 5(03): 217-220.

低体重早产儿内皮祖细胞体外血管生成能力研究

Study on angiogenic properties of endothelial progenitor cells in low birth weight preterm infants

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价