The effect of intestinal flora on catch-up growth of small for gestational age detected by high throughput sequencing of 16S rRNA gene in rats and its possible mechanism

doi:10.16150/j.1671-2870.2020.04.010

Abstract

Abstract:

Objective: To explore the effect of intestinal flora on catch-up growth in the rats of small for gestational age (SGA) and study the possible mechanism on catch-up growth (CUG) mediated by intestinal flora. Methods: SGA model was established by half dietary restriction during the pregnancy in female rats. A total of 54 baby rats were collected and were divided into two groups at the 4th week according to whether the rats achieved CUG or not: non-catch-up growth SGA (NCUG-SGA) group and CUG-SGA group. The serum insulin-like growth factor-1(IGF-1) in baby rats were measured with enzyme-linked immunosorbent assay (ELISA), the fecal intestinal flora was investigated by Illumina sequencing of 16S rRNA gene amplicons, and the level of fecal short-chain fatty acid (SCFA) and was tested by gas chromatography-mass spectrometer（GC-MS）, respectively. The difference in composition of the intestinal flora, the level of fecal SCFA and serum IGF-1 were compared between 2 groups. Results: The level of IGF-1 in CUG-SGA group was significantly higher than the NCUG-SGA group[（250.41±85.66） μg/L vs（112.29±32.26） μg/L, P<0.05] in baby rats. Simpson's diversity index for intestinal flora in CUG-SGA group was markedly higher than NCUG-SGA group. The level of fecal SCFA in CUG group was higher than NCUG group（P<0.05). SCFAs were correlated with relative abundance of 11 types of differentially expressed bacteria genus in CUG-SGA group（|r|>0.6, P<0.05）. Meanwhile,the levels of propionate, butyrate,isobutyrate and valerate were positively correlated with body weight and length as well as the serum level of IGF-1 in CUG-SGA rats. Conclusions: Intestinal flora exerts an effect on CUG in SGA rats. It may be achieved through regulating energy metabolism and absorption by SCFA,which produced by intestinal flora metabolism in the body.

Key words: Small for gestational age, Intestinal flora, Catch-up growth, Rat

CLC Number:

R725.7

AN Jingjing, WANG Junqi, XIAO Yuan, LU Wenli, LI Lin, WANG Wei, DONG Zhiya. The effect of intestinal flora on catch-up growth of small for gestational age detected by high throughput sequencing of 16S rRNA gene in rats and its possible mechanism[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(04): 375-380.

Figures/Tables 5

References 19

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组别	出生时		4周龄
组别	身长（cm）	体重（g）	身长（cm）	体重（g）
CUG-SGA组（n=30）	4.92±0.38	4.65±0.16	12.35±0.50	73.41±7.23
NCUG-SGA组（n=24）	4.65±0.51	4.54±0.18	11.61±0.27	55.87±2.67
P值	0.150	0.120	<0.001	<0.001

差异菌属	相对丰度		P值
差异菌属	NCUG-SGA组	CUG-SGA组	P值
乳酸杆菌属	25.42%	16.50%	0.028
颤螺菌属	3.29%	1.12%	0.009
未分类_瘤胃菌科	2.76%	0.19%	0.009
未分类_脱硫弧菌科	0.86%	0.45%	0.047
未分类_毛螺菌科	0.79%	0.22%	0.028
梭菌属	0.20%	0.27%	0.028
Odoribacter^*	0.14%	0.01%	0.007
厌氧棍状菌属	0.11%	0.00%	0.019
脱硫弧菌属	0.17%	0.06%	0.028
未分类_克里斯滕森菌科	0.10%	0.03%	0.009
Dehalobacterium^*	0.10%	0.03%	0.009
链球菌属	0.10%	0.31%	0.028
未分类_消化球菌科	0.06%	0.01%	0.009
嗜胆菌属	0.06%	<0.01%	0.005
Dorea^*	0.06%	<0.01%	0.005
罗斯伯里菌属	0.04%	0.17%	0.016
萨特菌属	0.03%	0.10%	0.047
未分类_链球菌科	0.01%	0.02%	0.044
未明确_细菌	0.01%	0.04%	0.028
未分类_梭菌科	<0.01%	0.02%	0.055
丁酸弧菌	<0.01%	0.04%	0.019
未明确_RF39^*	<0.01%	0.0%	0.019

组别（n）	乙酸	丙酸	丁酸	异丁酸	戊酸	异戊酸	己酸
NCUG-SGA组	3.49±0.33	1.71±0.07	1.20±0.25	0.01±0.00	0.07±0.03	0.08±0.03	0.03±0.00
CUG-SGA组	7.02±2.59	3.44±1.39	2.91±0.38	0.02±0.00	0.14±0.05	0.20±0.08	0.04±0.01
P值	0.017	0.025	<0.001	0.001	0.022	0.012	0.003