外科理论与实践 ›› 2021, Vol. 26 ›› Issue (05): 430-436.doi: 10.16139/j.1007-9610.2021.05.014
收稿日期:
2020-05-18
出版日期:
2021-09-25
发布日期:
2022-07-22
通讯作者:
王正林
E-mail:wang.zhenglin@zs-hospital.sh.cn
LU Weihui1, LIU Wei2, WANG Cong2, WANG Zhenglin2()
Received:
2020-05-18
Online:
2021-09-25
Published:
2022-07-22
Contact:
WANG Zhenglin
E-mail:wang.zhenglin@zs-hospital.sh.cn
摘要:
目的:探究CXC类趋化因子受体2(C-X-C motif chemokine receptor, CXCR2)对胃癌细胞侵袭、迁移及凋亡等生物学特性的影响。方法:取人胃癌MGC80-3细胞和SGC-7901细胞。转染建立CXCR2过表达细胞,质粒瞬时转染和RNA干扰建立敲减细胞。蛋白质印迹实验研究蛋白质相关细胞表型变化。通过transwell侵袭及迁移实验、增殖实验(cell counting kit-8, CCK8)、流式细胞实验Annexin Ⅴ-PI双染法以及实时定量PCR等进行细胞功能、增殖、凋亡以及CXCR2 mRNA表达的检测。结果:胃癌细胞过表达CXCR2后其侵袭、迁移能力显著增强(P<0.05),凋亡率显著降低(P<0.01)。CXCR2下调后抑制胃癌细胞的侵袭、迁移能力(P<0.05),凋亡率显著升高(P=0.02)。CXCR2过表达的胃癌细胞中Akt的磷酸化水平显著上调。Akt磷酸化水平与凋亡抑制蛋白Bcl-2的表达水平相一致。结论:CXCR2上调后增强胃癌细胞的侵袭、迁移及抗凋亡能力。胃癌细胞中CXCR2的抗凋亡能力与CXCR2/Akt/Bcl-2通路相关。
中图分类号:
陆伟辉, 刘威, 王聪, 王正林. CXCR2对胃癌细胞侵袭、迁移及凋亡的影响[J]. 外科理论与实践, 2021, 26(05): 430-436.
LU Weihui, LIU Wei, WANG Cong, WANG Zhenglin. Effects of CXCR2 on invasion, migration and apoptosis of gastric cancer cells[J]. Journal of Surgery Concepts & Practice, 2021, 26(05): 430-436.
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