Abstract: Objective To explore the feasibility of rapid identification of parathyroid with fluorescence microsphere immunochromatography during thyroid and parathyroid surgery. Methods Clinical data of 36 patients who underwent thyroid and parathyroid surgery by same surgical group in Department of Thyroid Surgery First Hospital of Jilin University from July 2017 to October were collected. The parathyroid and non-parathyroid tissue such as thyroid, fat tissue and lymph node which were resected were punctured by same operator using 1 mL needle. The T1 (test value) and C (control value) were measured by fluorescence microsphere immunochromatography. T1/C were compared between different tissues and T1/C of the punctured tissue compared with the pathological diagnosis. Results A total of 51 sets of data were obtained. T1/C of parathyroid was (3.653± 3.177) and T1/C of non-parathyroid tissue was (0.056± 0.027) with statistically significant diffe-rence(P< 0.01). T1/C of parathyroid was significantly higher from that of non-parathyroid tissue including thyroid, lymph node and fat tissue (P< 0.05). There was no significant difference between T1/C of parathyroid and parathyroid adenomas (P> 0.05). The results of immunofluorescence test to identify parathyroid had sensitivity (true positive rate) 95.7% (22/23), specificity (true negative rate) 100% (28/28), false positive rate (misdiagnosis rate) 0 (0/28), false negative rate (omission diagnostic rate) 4.4% (1/23) and accuracy 98.0% (50/51). Conclusions Fluorescence microsphere immunochromatography can be used to identify parathyroid in real-time mode during surgery with high sensitivity and specificity, which is a convenient procedure and valuable to be recommended.

Key words: Parathyroid, Thyroid, Fluorescence microsphere immunochromatography, Thyroid surgery