组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (1): 5-9.doi: 10.3969/j.issn.1673-0364.2019.01.002

• 论著 • 上一篇    下一篇

BK通道抑制剂对人脐带华通胶来源的间充质干细胞的增殖、凋亡及细胞因子的影响

宋阿会，佟琰，刘英莉   

  1. 上海交通大学医学院附属第九人民医院肾内科
  • 收稿日期:2018-11-20 修回日期:2019-01-07 出版日期:2019-02-20 发布日期:2019-02-20
  • 作者简介:刘英莉(E-mail:18616375719@163.com)。

Effectiveness of BK Channel Inhibitor on Proliferation,Apoptosis and Cytokines of Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton's Jelly

SONG Ahui,TONG Yan,LIU Yingli   

  1. Department of Nephrology,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)
  • Received:2018-11-20 Revised:2019-01-07 Online:2019-02-20 Published:2019-02-20
  • Contact: 上海市浦江人才计划(2016PJD032)

摘要： 目的 探究BK通道抑制剂对人脐带华通胶来源的间充质干细胞(WJ-MSC)的细胞活性、细胞凋亡、细胞因子分泌的影响。方法 以组织贴壁法分离培养人WJ-MSC,Western-blot检测BK通道的表达;用不同浓度BK通道抑制剂处理后,CCK-8法检测细胞活性,Annexin-V-FITC/PI检测细胞凋亡,Elisa检测细胞因子(IL6、IL10)的表达。结果 人WJ-MSC上存在BK通道的表达,BK通道抑制剂能够明显降低细胞活力(P<0.05);促进细胞凋亡(P<0.05);促进细胞因子(IL6)的分泌(P<0.05),但并不增加IL10的分泌。结论 BK通道抑制剂能够抑制人WJ-MSC的活性,促进细胞凋亡,促进IL6的分泌。

关键词: BK通道, 人脐带华通胶来源间充质干细胞, 细胞活性, 细胞凋亡, 细胞因子

Abstract: Objective To explore the effects of BK channels inhibitors on cell activity,apoptosis and cytokine secretion of mesenchymal stem cells derived from human umbilical cord Wharton's jelly(WJ-MSC).Methods The WJ-MSC were isolated and cultured by tissue adherent method.The expression of BK channels was detected by Western-blot.After treated with different concentrations of BK channels inhibitors,the activity of cells was detected by CCK-8,the apoptosis of cells was detected by Annexin-V-FITC/PI,and the expression of cytokine(IL6,IL10)was detected by Elisa.Results BK channels expression was found in WJ-MSC.BK channels inhibitors reduced cell activity,promoted cell apoptosis and secretion of cytokine(IL6),all of which have statistical significance(P<0.05).But BK channels inhibitors did not increase the secretion of IL-10.Conclusion BK channels inhibitors can inhibit the activity of WJ-MSC,promote cell apoptosis and promote the secretion of IL6.

Key words: BK channel, Mesenchymal stem cells derived from human umbilical cord Wharton's jelly, Cell activity, Cell apoptosis, Cytokines

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