组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2011, Vol. 7 ›› Issue (1): 20-29.doi: 10.3969/j.issn.1673-0364.2011.01.005

• 论著 • 上一篇    下一篇

骨髓间充质干细胞结合藻酸盐治疗椎间盘退变的实验研究

巢玉柳，金永，卞敏凯，张亮，蒋纯志   

  1. 常州市第三人民医院骨科；南京医科大学附属第一医院骨科
  • 发布日期:2020-07-23

Experimental Study of Degenerative Intervertebral Disc Treated by Bone Mesenchymal Stem Cells Combining Alginate in Rabbit

CHAO Yuliu1,JIN Yong1,BIAN Minkai1,ZHANG Liang1,JIANG Chunzhi2   

  1. 1 Department of Orthopedics,Changzhou Third People′s Hospital,Changzhou 213001,China;2 Department of Orthopedics,The First Affiliated Hospital with Nanjing Medical University,Nanjing 210029,China.
  • Published:2020-07-23

摘要： 目的观察骨髓间充质干细胞(Bone mesenchymal stem cell,BMSC)结合藻酸盐凝胶支架,治疗兔椎间盘退变的效果。方法将15只新西兰大白兔建立腰椎间盘退变模型,随机分为对照组、空白组和治疗组。体外培养兔BMSC,治疗组椎间隙注射BMSC结合藻酸盐凝胶支架,对照组注射藻酸盐凝胶,空白组注射生理盐水。应用核磁共振、免疫组化和生化分析,观察椎间盘退变的修复效果。结果治疗组影像学、病理组织学观察,以及蛋白多糖和Ⅱ型胶原的含量,均明显优于对照组和空白组,差异有统计学意义。结论 BMSC结合藻酸盐凝胶支架可用于治疗兔腰椎间盘退变。

关键词: 骨髓间充质干细胞, 藻酸盐, 椎间盘, 退变

Abstract: Objective To evaluate the effects of bone mesenchymal stem cells combining alginate on treatment of the degenerative intervertebral disc.Methods Fifteen New Zealand white rabbits were punctured in the vertebral gap to set up intervertebral disc degeneration model and were randomly divided into three groups,control group,blank group and experimental group.The rabbits were injected bone mesenchymal stem cell combining alginate gel stents in experimental group,alginate gel in control group,saline in blank group.The effect of repair was observed by MRI,immunohistochemical and biochemical analysis.Results Disc height,T2 value and the content of proteoglycan and Type Ⅱ collagen were higher in experimental group than in control and blank group.There was significant difference(P<0.05).Conclusion BMSC combining alginate could be used to repair the degenerative disc.

Key words: Bone mesenchymal stem cell, Alginate, Intervertebral disc, Degeneration.

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