Journal of Tissue Engineering and Reconstructive Surgery ›› 2010, Vol. 6 ›› Issue (5): 241-243.doi: 10.3969/j.issn.1673-0364.2010.05.001

• Original article •     Next Articles

Primary Study of the Expression of Embryonic Stem Cell Markers CD31 and SSEA-1 in Developing Embryos and Adult Tissues

WANG Shujun,FU Wei,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery,Shanghai Ninth People′s Hospital,Shanghai Jiaotong University School of Medicine,National Tissue Engineering Center,Shanghai 200011,China.
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划(2005CB522705)，国家自然科学基金(30571925,30671051)，上海市曙光人才计划(06SG22)，上海交通大学博士点创新基金(BXJ0920)

Abstract: Objective To detect the expression of CD31 and SSEA-1 at different stages of developing embryos,as well as their expressions in neonates,adult bone marrow and testis.Methods 129/SV mice were mated.Embryos at 10.5 postcoitus（E10.5）,14.5 and 18.5 days were collected.In addition,neonatal mice,bone marrow and testis from 6-week-old mice were also collected.Single cell suspension was achieved by digestion with collagenase.After anti-CD31 and anti-SSEA-1 antibody labeling,cells were analyzed by flow cytometry.Results CD31 and SSEA-1 were expressed in E10.5,E14.5 and E18.5.The expression levels were decreased with the development of embryos.Meanwhile,both markers were observed in neonates,adult bone marrow and testis,but the level was lower than in embryos.Conclusion A certain amount of CD31 and SSEA-1 expressing cells are existing in developing embryos as well as in adult tissues.

Key words: CD31, SSEA-1, Embryo, Bone marrow, Testis

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