Abstract: Objective To explore whether adipose-derived stem cells(ADSCs)can effectively exert its paracrine function in heterologous acellular dermal matrix(HADM)microenvironment and consequently improve the efficacy of HADM after implantation in vivo.Methods ADSCs were seeded on HADM and microplate.Total RNA of ADSCs from both materials were collected respectively,and qRT-PCR was used to evaluate the levels of genes associated with ADSC paracrine.In addition,HADM with and without ADSC pre-culture were implanted on the back of rats.All samples were harvested 4 weeks after operation for histological staining.Results The expression of genes with respect to inflammatory regulation(TGF-β,iNOS,TSG-6,COX-2),pro-angiogenesis(VEGF,HGF,bFGF)and stemness(SOX-2)increased when ADSCs were seeded on HADM compared to ADSCs seeded on microplate.After pre-culture,in vivo implanted ADSCs-HADM exhibited improved vascularization and mitigated inflammatory response compared to untreated HADM.Conclusion HADM can provide a beneficial microenvironment for ADSCs to exert their paracrine function.After pre-culture with ADSCs in vitro,the vascularization rate of in vivo implanted HADM is accelerated and the inflammatory response is attenuated and localized.

Key words: Heterologous acellular dermal matrix, Adipose-derived stem cells, Pre-culture, Paracrine