Abstract: Objective To evaluate the effects of bone mesenchymal stem cells combining alginate on treatment of the degenerative intervertebral disc.Methods Fifteen New Zealand white rabbits were punctured in the vertebral gap to set up intervertebral disc degeneration model and were randomly divided into three groups,control group,blank group and experimental group.The rabbits were injected bone mesenchymal stem cell combining alginate gel stents in experimental group,alginate gel in control group,saline in blank group.The effect of repair was observed by MRI,immunohistochemical and biochemical analysis.Results Disc height,T2 value and the content of proteoglycan and Type Ⅱ collagen were higher in experimental group than in control and blank group.There was significant difference(P<0.05).Conclusion BMSC combining alginate could be used to repair the degenerative disc.

Key words: Bone mesenchymal stem cell, Alginate, Intervertebral disc, Degeneration.