A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone

doi:10.3969/j.issn.1673-0364.2009.06.001

Abstract

Abstract: Objective To explore the feasibility of human bone marrow stromal cells(hBMSCs) and its clinical repair of limb bone defects.Methods Nine patients of bone defects(5 bone tumors,4 bone defects) were included in this study.The hBMSCs were isolated by Percoll gradient centrifugation with bone marrow aspirated from iliac crest of each patient.hBMSCs were cultured in vitro and induced to become osteogenic cells in the DEME medium containing 10% self-serum,β-glycerophosphate(10 nmol/L) dexamethasone(10-8 mol/L),L...

Key words: Bone marrow stromal cell, Bone defect, Tissue engineering bone

CLC Number:

R687.3+4
R738.1

ZHU Lian,ZOU Cheng,ZHU Min,CUI Lei,CAO Yilin. A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(3): 121-124.

[1]	KAN Tianyou(阚天佑), XIE Kai(谢凯), QU Yang(曲扬), AI Songtao (艾松涛), JIANG Wenbo (姜闻博), WU Haishan (吴海山), WANG Liao(王燎), YAN Mengning(严孟宁). 3D-Printed Porous Titanium Augments for Reconstruction of Massive Bone Defect in Complex Revision Total Knee Arthroplasty: Implant Design and Surgical Technique [J]. J Shanghai Jiaotong Univ Sci, 2021, 26(3): 334-338.
[2]	JIN Xin,ZHANG Zhiliang,LU Yi,HUANG Yixiong,FAN Zhihong,CHEN Rui. Experimental Study on the Osteogenic Induction by Transwell Co-Culture of Rat BMSCs and ADSCs in Vitro [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(5): 322-324.
[3]	PENG Yiyu,WANG Zi,YUAN Qingyue,GU Ping,BI Xiaoping. An Experimental Study of a Biomineralized Collagen Electrospinning Scaffold for Guided Bone Regeneration of Calvaria Defect in Rats [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019, 15(2): 65-71.
[4]	HAN Qianqian,XUE Bin,WANG Han,SHI Jianfeng,WANG Ying,TANG Jinglong,WANG Chunren. Research Progress of Material for Bone Tissue Engineering [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2017, 13(6): 343-345.
[5]	ZHANG Haifeng,DU Zijing,JIANG Wenbo,HAN Dong. Biocompatibility Research of Three-dimensional Printed Polylactic acid/Hydroxyapatite Composite with Bone Marrow Stromal Cells in vitro [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2015, 11(6): 349-353.
[6]	WU Xiaowei, WANG Qian, CAO Yilin, XIAO Ran. Vascularization Strategy of Tissue Engineered Bone [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2015, 11(5): 331-334.
[7]	ZHAO Junjie,ZHU Xiaofeng,YAN Fuhua. hVEGF Gene Modified Tissue-engineered Compound in the Reconstruction of Periodontal Tissue Defects in SD Rats [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2014, 10(5): 250-254.
[8]	XIONG Zhuyou,FANG Xiaokui,XU Jing,ZHANG Li,WANG Chen,LI Guangzao. Allogeneic Adipose Derived Stem Cells Combining with Demineralized Bone Matrix for Repairing of Rat Ulnar ;Defects [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2013, 9(5): 245-270.
[9]	LIU Chen,Yang Qingsong,SU Jiacan. Advancement of Drug-Delivery System in the Treatment of Osteomyelitis with Bone Defects [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2012, 8(2): 117-120.
[10]	ZHANG Dawei,TIAN Qingye,LIU Guangjun,LIU Xuetao,WANG Qian,YANG Lei. Bone Allograft Embedded by Vascularized Periosteum Flap for the Reconstruction of Segmental Bone Defect [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2012, 8(1): 26-31.
[11]	ZHANG Chao,QU Weidong,ZHAO Huaqiang. Experimental Study of Bone Marrow Stromal Cells and Induced Endothelial Cells in Co-Culture under Hypoxia [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2010, 6(6): 315-318.
[12]	ZHOU Xiao,CAO Yilin,CUI Lei,YU Jianjun,LI Zan,CHEN Jie,ZUO Chaohui,LIU Guangpeng,MO Yi,HU Bingqian,WU Shengqi. Repair of Mandibular Defect by Using Tissue Engineered Bone:A Report of 3 Cases [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2010, 6(4): 183-187.
[13]	HUANG Yongling,YAN Fuhua,ZHONG Quan,ZHAO Xin. Effects of Media on the Proliferation and Differentiation of Bone Marrow Stromal Cells in Beagle Dogs [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(4): 181-185.
[14]	WANG Yougang,LI Xinqing,LI Chunhe,WU Zhiyuan,CUI Lei,LIU Guangpeng,LI Yulin,SUN Jian. Clinical Application of Auto-hBMSCs Acting as Seeding Cell for Tissue Engineered Bone on the Cranial Defects [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(3): 125-127.
[15]	LOU Xiao,WANG Jinsong,JIN Jide,HE Ziming,WU Zuze,GUO Zikuan. Platelet Lysates Enhance the Proliferation of Human Bone-Marrow Stromal Cells In Vitro [J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(2): 61-64.

A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments