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23 July 2020, Volume 5 Issue 3 Previous Issue    Next Issue

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A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone ZHU Lian,ZOU Cheng,ZHU Min,CUI Lei,CAO Yilin 2009, 5 (3):  121-124.  DOI: 10.3969/j.issn.1673-0364.2009.06.001 Abstract ( 348 )

Objective To explore the feasibility of human bone marrow stromal cells(hBMSCs) and its clinical repair of limb bone defects.Methods Nine patients of bone defects(5 bone tumors,4 bone defects) were included in this study.The hBMSCs were isolated by Percoll gradient centrifugation with bone marrow aspirated from iliac crest of each patient.hBMSCs were cultured in vitro and induced to become osteogenic cells in the DEME medium containing 10% self-serum,β-glycerophosphate(10 nmol/L) dexamethasone(10-8 mol/L),L... Related Articles | Metrics

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Clinical Application of Auto-hBMSCs Acting as Seeding Cell for Tissue Engineered Bone on the Cranial Defects WANG Yougang,LI Xinqing,LI Chunhe,WU Zhiyuan,CUI Lei,LIU Guangpeng,LI Yulin,SUN Jian 2009, 5 (3):  125-127.  DOI: 10.3969/j.issn.1673-0364.2009.06.002 Abstract ( 510 )

Objective To investigate the clinical effect of hBMSCs acting as seeding cell for tissue engineer bone on the cranial defects.Methods From June 2006 to February 2007,four patients with traumatic cranial defect were treated by using auto-hBMSCs acting seeding cell for tissue engineered bone.Autogenic hBMSCs were isolated,proliferated and induced in vitro,then combined with partially demineralized bone matrix.After co-cultured for 1 week,the cell/scaffold complexes were used for implantation.Gross observation... Related Articles | Metrics

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An Experimental Study on Reconstruction of Acellular Dermal Matrix Carried PDGF-BB and IGF-1 LIU Cheng,FU Jinsong,LIU Shan,ZHOU Zengding,SONG Zhiyong 2009, 5 (3):  128-130.  DOI: 10.3969/j.issn.1673-0364.2009.06.003 Abstract ( 369 )

Objective To explore the reconstruction of acellular dermal matrix carried PDGF-BB and IGF-1 in vitro.Also investigate cultured human fibroblast proliferation and collagen production affected by constructed acelluar dermal matrix.Methods The degraded micro beads with PDGF-BB and IGF-1 were synthe-sized and connected to acellular dermal matrix.Acelluar dermal matrix was cultured with fibroblast.Fibroblast proliferation rate and cell variability were observed by cell count and WST-8 colorimeter;The release of... Related Articles | Metrics

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Experimental Study on Tissue Engineered Blood Vessel with Bioreactor XU Zhicheng,ZHANG Wenjie,LI Hong,ZHOU Guangdong,CUI Lei,LIU Wei,CAO Yilin 2009, 5 (3):  134-137.  DOI: 10.3969/j.issn.1673-0364.2009.06.005 Abstract ( 347 )

Objective To investigate the feasibility of constructing tissue engineered blood vessels with bioreactor.Methods Cell-PGA(polyglycolic acid) complex was constructed by seeding smooth muscle cells(SMCs) isolated from canine carotid artery on PGA unwoven fibers.The cell-PGA complex was cultured in a bioreactor(pulse rate:75 /min,radical distension<5%).Eight weeks after culture in vitro,Endothelial cells(ECs) isolated from canine carotid vein were seeded on PGA and cultured for another 5 days.The control gr... Related Articles | Metrics

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Research Biocompatibility of the Poly(l-lactic acid) Nano-fibrous Scaffolds with Spinal Cord Tissues in Rats ZHANG Yanqing,ZENG Yuanshan,HE Liumin,QUAN Daping,LI Yan 2009, 5 (3):  143-147.  DOI: 10.3969/j.issn.1673-0364.2009.06.007 Abstract ( 527 )

Objective To explore the biocompatibility of the Poly(l-lactic acid)(PLLA) nano-fibrous scaffold with spinal cord tissue of rats.Methods PLLA three-dimensional(3D) scaffolds with macro/micro-pores and nano-fibrous structure were fabricated by phase separation from a ternary PLLA/dioxane/water system.MSCs-PLLA nano-fibrous scaffolds complex were constructed in vitro.Twelve SD rats were divided into two groups:control injured group and MSCs transplanted group.T10 spinal cord segment was exposed,and then trans... Related Articles | Metrics

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Experimental Study on Biological Characteristics of the Wollastonite Coating for Implant Applications SHEN Xia,XU Huashun,LIU Shiqiang,PANG Jingsong 2009, 5 (3):  148-149.  DOI: 10.3969/j.issn.1673-0364.2009.06.008 Abstract ( 345 )

Objective To evaluate the bioactivity and biocompatibility of the wollastonite coating(CaSiO3) and to provide the experimental basis for the clinical choice on the transformation methods of implant surfaces.Methods Six dogs were randomly divided into two groups.And 24 implants(12 sprayed with the hydroxyapatite coating,12 sprayed with the wollastonite coating) were randomly implanted into the mandibula of the dogs.The shearing bond stress of interface between implant and bone was measured with push-test.The... Related Articles | Metrics

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Experimental Study on Compression Facial Nerve Injury in Rabbits SHEN Chen,GUO Zhilin,OUYANG Huoniu 2009, 5 (3):  150-152.  DOI: 10.3969/j.issn.1673-0364.2009.06.009 Abstract ( 596 )

Objective To study posttraumatic regeneration of the facial nerve in rabbits.Functional and histological analysis were compared with normal nerves on opposite sides to finding the suitable opportunity to treat the peripheral facial paralysis on clinic.Methods The left facial nerve trunks of 15 rabbits were selected to compression lesion and the right sides of them serve as the control groups.The rabbits were killed at 2,4 and 6 weeks after the operation,respectively.The obtained specimens were observed macr... Related Articles | Metrics

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Clinical Application of Perforator Flaps for the Reconstruction of Perforating Defects in the Head and Neck DAI Jie,ZHOU Xiao,CHEN Jie,YU Jianjun,LI Zan 2009, 5 (3):  153-155.  DOI: 10.3969/j.issn.1673-0364.2009.06.010 Abstract ( 324 )

Objective To explore the clinical application of free perforator flaps in the reconstruction of perforating defects following the ablation of head and neck tumor.Methods From Jan.2006 to Mar.2008,29 patients with perforating defects in the head and neck were treated by free perforated flaps.Results Among 29 free perforator flaps,vascular crises occurred in 2 cases.One flap was partly necrosis.Early local complications occurred in 6 cases.Patients were followed up for 3-24 months(14 months on average).The fi... Related Articles | Metrics

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Reconstructive Techniques for the Repair of Secondary Defect of Unilateral Cleft Lip LI Dajun,GAO Xuehong,ZHANG Lin,NING Jinlong,LI Xiaojing 2009, 5 (3):  156-158.  DOI: 10.3969/j.issn.1673-0364.2009.06.011 Abstract ( 344 )

Objective To evaluate the different techniques for the repair of secondary defect of unilateral clift lip.Methods The incision line was designed along the nasal column and the alar cartilage was dissected.The inner crus of nasal alar were cut and fixed at same height with normal side.A part of epithelium of the nasal base was removed and a rhombus flap was formed.It was put into deep cavity of nasal base.The wide nasal base and abduct crus of nasal alas were corrected by V-Y or Y-V advanced flaps.Results Sa... Related Articles | Metrics

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The Modified Langenbeck s Technique Combined with Limberg s Technique and Levator-muscle Reconstruction in the Treatment of the Unilateral Complete Cleft Palate LIN Genhui,ZHENG Qingjiang,DING Li,XIONG Shuyuan 2009, 5 (3):  159-160.  DOI: 10.3969/j.issn.1673-0364.2009.06.013 Abstract ( 342 )

Objective To evaluate the clinical effect of the modified Langenbeck s technique combined with Limberg s technique and Levatormuscle reconstruction in the treatment of the unilateral complete cleft palate.Methods From 2003-2008,58 patients with the unilateral complete cleft palate were collected of our department,they were treated by the modified Langenbeck s technique combined with Limberg s technique and Levator-muscle reconstruction.All patients were followed up for 3-48 months.The situation of the palat... Related Articles | Metrics

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The Treatment for Repeating Effusion after Injecting Augmentation Mammaplasty ZHANG Bo,WANG Jian,YU Li,ZHU Chang 2009, 5 (3):  161-162.  DOI: 10.3969/j.issn.1673-0364.2009.06.012 Abstract ( 315 )

Objective To investigate the operative method and clinical results of repeating after augmentation mammaplasty with injecting hydrophilic polyacrylamide gel.Methods The reason of the repeating effusion was analyzed.The injected hydrophilic polyacrylamide gel was taken out and the fibrous capsule around the injection substance was removed.Results All patients were satisfied with the results.There were no recurrence of effusion after 18 months follow up.Conclusion In the treatment of repeating effusion after ... Related Articles | Metrics

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Clinical Observation About Percutaneous Plate Osteosynthesis with external Fixator for Distal Tibia Fractures ZHANG Wei,ZOU Jian,ZHANG Changqing 2009, 5 (3):  163-165.  DOI: 10.3969/j.issn.1673-0364.2009.06.014 Abstract ( 251 )

Objective To summarize the clinical use of minimally invasive percutaneous plate osteosynthesis(MIPPO) with external fixator in the treatment of distal tibia fractures and to investigate its clinical effects.Methods From September 2004 to April 2005,32 patients suffered from closed distal tibia fractures were selected to this study.There were 28 males and 4 females,aged 21-45 years old(32.6 years on average).All the patients were treated delayed operation.The first step was indirect reduction by unilateral ... Related Articles | Metrics