Table of Content

23 July 2020, Volume 13 Issue 2 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Original article

Select

Fabrication and Biocompatibility Evaluation of Collagen/Chitosan Scaffolds Embedded with Simvastatin-Loaded PLGA Microspheres LI Yu, ZHANG Zhanzhao, WU Dingyu, LI Qiannan, Geng Yingnan, CAO Yilin, LIU Wei, ZHANG Wenjie, ZHOU Guangdong, WANG Xiansong, ZHANG Zhiyong 2017, 13 (2):  61-65.  DOI: 10.3969/j.issn.1673-0364.2017.02.001 Abstract ( 418 )   Objective To prepare a collagen/chitosan scaffold incorporating simvastatin-loaded PLGA microspheres (SIM/COL/CS), which can release simvastatin (SIM) in a sustained manner, and investigate the biocompatibility of the scaffold. Methods Collagen/chitosan scaffolds incorporating SIM-loaded PLGA microspheres were prepared by combining freeze-drying technology and emulsion-solvent evaporation method. The morphology and drug release profile of the scaffolds were characterized by scanning electron microscope and ultraviolet spectrophotometry respectively. The effects of the composite scaffold on the proliferation and viability of the rat bone marrow-derived mesenchymal stem cells (BMSCs) were assessed by Cell Counting Kit 8 (CCK-8) and Live/Dead cell imaging kit separately. The osteoinductive effect of the scaffold on BMSCs was evaluated by alkaline phosphatase (ALP) activity assay and alizarin red S staining. Results The composited scaffolds exhibited an interconnected porous structure and the PLGA microspheres were tightly integrated within the matrix. Besides,the SIM exculpated in the scaffold was released in a sustained manner for up to 27 days. CCK-8 assay showed that although there was no difference in cell number between the COL/CS and SIM/COL/CS groups on days 1, 3 and 5, cell number in the SIM/COL/CS group was significantly higher than that in the COL/CS group on day 7. Live/dead cell staining indicated the viability of the BMSCs on both COL/CS and SIM/COL/CS were good and few dead cells (red) was found. Moreover, both of the ALP activity and calcium deposit in the SIM/COL/CS group were significantly higher than those in the COL/CS group. Conclusion COL/CS scaffolds containing SIM-loaded PLGA microspheres for controlled SIM delivery can be successfully prepared by combining freeze-drying technology and emulsion-solvent evaporation method. The novel SIM-loaded COL/CS scaffold can promote both the proliferation and osteogenic differentiation of BMSCs and has great application potentials in bone repair. Related Articles | Metrics

Select

The Feasibility of the Macroporous GT/PCL Electrospun Material as Scaffold for Cartilage Tissue Engineering ZEHNG Rui, ZHAO Shifang, ZHU Yueqian, ZHOU Guangdong 2017, 13 (2):  66-69.  DOI: 10.3969/j.issn.1673-0364.2017.02.002 Abstract ( 357 )   Objective To investigate the feasibility of the macroporous gelatin/polycaprolactone (GT/PCL) electrospun material as scaffold for cartilage tissue engineering. Methods The auricular chondrocytes of porcine were harvested as seed cells. Two kinds of electrospun membrane were prepared and compared: the macroporous membranes (experimental group) and the non-woven membranes (control group) as scaffolds. The structure and porosity of the two materials were observed. Adhesion and proliferation of chondrocytes on the two membranes were examined in two-dimensional level (2D). And the depth of cellular infiltration were observed in three-dimensional level (3D). Results Scanning electron microscope (SEM) results suggested that the pore size of the experimental group was significantly bigger than the control group, and the data of porosity supported this result. In 2D level, there was no significant difference in adhesion and proliferation of chondrocytes between the two groups. However, SEM showed that chondrocytes grew into the interior of the macroporous material. In 3D level, the infiltration depth of chondrocytes in the experimental group was significantly greater than that in the control group. Conclusion The macroporous GT/PCL membranes do not affect the adhesion and proliferation of chondrocytes, and it is beneficial to the growth and infiltration of chondrocytes. It could be a promising scaffold for cartilage tissue engineering. Related Articles | Metrics

Select

The Inhibition of iRGD-Exosomes-Doxorubicin on the Proliferation of Fibroblasts in Vitro HU Huan,ZHENG Jianghong 2017, 13 (2):  70-81.  DOI: 10.3969/j.issn.1673-0364.2017.02.003 Abstract ( 439 )   Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of the proliferation of fibroblasts. Methods The exosomes were extracted from the iRGD-transfected fibroblasts. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to fibroblasts. The ability of iRGD-exo-dox on inhibiting the proliferation of fibroblasts was analyzed. Results The affinity of iRGD-exo-dox to fibroblasts was higher than the blank-exo-dox. The proliferation of fibroblasts was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of fibroblasts by targeting function, which can be used in the treatment of keloid. Related Articles | Metrics

Select

Pathologic Characters of the Nerve with Adipose Infiltration in Type Ⅱ Macrodactyly YANG Xi,JIANG Yongkang,ZHOU Shengbo,WANG Bin 2017, 13 (2):  73-76.  DOI: 10.3969/j.issn.1673-0364.2017.02.004 Abstract ( 569 )   Objective To explore the ultra-structural changes and functional protein expression of enlarged nerves in patients with type Ⅱ macrodactyly. Methods The ultra-structure of the enlarged nerves in type Ⅱ macrodactyly patients was observed under electron microscope. The expression of S100, LN and H3K27me3 were evaluated by immune-fluorescence staining. Normal digital nerves from polydactyly patients were used as control. Results The number of myelinated fibers was significantly decreased, and some myelinated lamellar structures were shrunk and destroyed. The number and morphology of Schwann cells did not change significantly. There was no significant difference in the expression of S100 between the macrodactyly group and control group. LN was low expressed in the normal digital nerve, and in the enlarged nerve samples, LN showed a stronger expression. In addition, the expression of H3K27me3 in both enlarged nerve tissue and normal nerve tissue were positive. Conclusion The neurofibromatosis of type Ⅱ macrodactyly is benign hyperplasia. In patients with type Ⅱ macrodactyly, the sheath of the enlarged nerves was damaged. However, LN positive staining indicates a repair mechanism to compensate for nerve injury. Therefore, the neurosurgical procedures should be determined according to the neurological damage and compensatory situation in patients in typeⅡmacrodactyly. Related Articles | Metrics

Select

Effects of Crocin on Photoaged Skin Fibroblasts DENG Mingwu,ZHANF Yicheng,LI Dong,YU Ziyou,WANF Xiangsheng,ZHANG Wenjie 2017, 13 (2):  77-81.  DOI: 10.3969/j.issn.1673-0364.2017.02.005 Abstract ( 432 )   Objective To investigate the protective effects of crocin on photoaged skin fibroblasts, and elucidate the mechanism of crocin on anti-photoaging, which could provide new evidence for clinical application of crocin. Methods 1. Human skin fibroblasts were isolated and treated with different concentration of crocin (0, 50, 100, 100μM) for 72 hours. CCK-8 assay was performed to detect cell proliferation. 2. Fibroblasts were irradiated with different doses of ultraviolet light (25, 50, 100 mJ/cm2), cell proliferation was measured by CCK-8 kit after 72 hours. 3. Fibroblasts were treated with different concentration of crocin (0, 50, 100, 100μM) for 24 hours and irradiated by ultraviolet light (100 mJ/cm2). Intracellular reactive oxygen species (ROS) were analyzed immediately by flow cytometry. Cell cycles were analyzed 48 hours after irradiation by flow cytometry. Seventy-two hours after treatment, cell proliferation was measured by CCK-8 kit, total RNA was extracted, and the expression of type Ⅰ collagen were detected by RT-PCR. Results 1. Low concentration of crocin (50, 100 μM) had no significant effect on the proliferation of skin fibroblasts while high concentration of crocin (200μM) promoted cell proliferation. 2. Ultraviolet irradiation inhibited the proliferation of skin fibroblast in a dose dependent manner. 3. Ultraviolet irradiation enhanced cellular level of ROS, and inhibited cell proliferation and the expression of typeⅠcollagen. Crocin (12.5, 25, 50, 100μM) treatment reduced the production of ROS caused by ultraviolet irradiation, and restored the cell cycle, cell proliferation, and type I collagen expression. Conclusion Crocin prevent the photoaging of dermal fibroblasts against ultraviolet irradiation by reducing the cellular level of ROS and restoring the cell cycle and type I collagen expression. Related Articles | Metrics

Select

Surgical Treatment of Scalp Neurofibromas WEN Guozhong,YUAN Siming,WANG Min,XU Yuan,HAI Xi,CUI Lei,WANG Jun,HONG Zhijian 2017, 13 (2):  82-84.  DOI: 10.3969/j.issn.1673-0364.2017.02.006 Abstract ( 689 )   Objective To explore the individual surgical treatment strategy for neurofibroma. Methods A retrospective analysis was made on the 35 cases of scalp neurofibroma treated in our clinic from January 2006 to June 2016. The size of the tumor, surgical treatments and results were recorded. The surgical treatments included: ① If the tumor diameter was less than 5 cm, tumor resection and local skin flap transfer was performed. ② If the diameter was more than 5cm, skin expander treatment was preferred. And if the patient refused to use the expander, then tumor resection and skin graft was performed instead. ③ If the tumor was very large or hemorrhage occurred in the tumor, preoperative arteriography and superselective embolization of the tumor's nutrient arteries were conducted, and he tumor resection was performed within 3 days after the embolization. Results Fourteen patients were treated with tumor resection and local skin flap transfer. 9 patients were treated with skin expander. 7 patients were treated with tumor resection and skin graft. 5 patients were treated with preoperative arterial embolization, tumor resection and skin graft. All tumors were successfully removed without intraoperative hemorrhage or other life-threatening complications. Marginal necrosis in partial flap, small area of skin graft which failed to survive, were both healed with dressing changing. All the patients were followed up for 6 months to 3 years, no recurrence was observed. Conclusion For the scalp neurofibroma, individualized surgical treatment selection according to the conditions of the tumor (such as the size, blood supply, intra-tumor hemorrhage, etc.) can ensure the intraoperative safety, preserve the scalp appearance, and so get good results. Related Articles | Metrics

Select

Plastic and Reconstructive Surgery for Severe Facial Deformity of Hemihyperplasia XIE Fang,XU Jiajie,LU Jianjian,ZHANG Chao,YANG Liya,LI Shuyuan,SUN Heng,TENG Li 2017, 13 (2):  85-92.  DOI: 10.3969/j.issn.1673-0364.2017.02.007 Abstract ( 348 )   Objective To explore the clinical efficacy of reconstructive surgery in severe facial deformity of hemihyperplasia. Methods From September 2003 to December 2014, 5 cases of hemihyperplasia with severe facial deformities were treated with integrated application of facial liposuction, resection of lipoma, malar bone and zygomatic arch grinding, spat suspension and so on. Results All the patients were followed up for 12 to 24 months, postoperative facial contour was significantly improved, and the postoperative facial expression was not affected. Conclusion Integrated reconstructive treatments for severe facial deformity of hemihyperplasia could greatly improve the facial symmetry, and has no affection on the postoperative facial expression. Related Articles | Metrics

Select

Ear Reconstruction of Low-Set Earlobe Type Microtia Using Remnant Earlobe Retrograde Transposition SUN Hengyun,ZHAO Yanyong,JIANG Haiyue,YANG Qinghua,HE Leren,PAN Bo,LIN Lin,LIU Ying,HAN Jingjian 2017, 13 (2):  87-89.  DOI: 10.3969/j.issn.1673-0364.2017.02.008 Abstract ( 435 )   Objective To explore the clinical application of remnant earlobe retrograde transposition in the ear reconstruction for microtia with severe low-set ear. Methods From January 2014 to December 2015, 20 cases of unilateral microtia with severe low-set ear were treated. Ear reconstruction was processed with expansion technique. A 50 mL kidney-shaped expander was implanted inside retroauricular region. Saline injection was carried out three times a week. It took one month to complete the saline injection. One month after expansion, ear reconstruction was carried out secondarily. The lower part of the ear remnant was incised to form an earlobe remnant flap, which was retrogradely transposed to reconstruct earlobe. The wound of ear remnant was sutured afterwards. Results No blood supply compromise occurred in 20 cases. All the patients were followed up for 6 months to 2 years, natural appearance and symmetry of bilateral earlobe position were gained. Conclusion In the cases of severe hemifacial microsomia with significant low-set ear, earlobe retrograde transposition can be used to correct the earlobe position and reconstruct earlobe with natural appearance and good symmetry with the other side. Related Articles | Metrics

Select

Nonsurgical Comprehensive Rhinoplasty:Clinical Observation of 56 Cases JIANG Zhengzheng,LIU Mingzhang,LIU Zhaofeng,YANG Jueping,JIANG Weitai,LIU Kai 2017, 13 (2):  90-92.  DOI: 10.3969/j.issn.1673-0364.2017.02.009 Abstract ( 390 )   Objective To investigate the effect of minimally invasive, nonsurgical comprehensive rhinoplasty technique. Methods From Jan. 2015 to Jan. 2016, 56 cases were treated with nonsurgical comprehensive rhinoplasty technique, including Hyaluronic acid injection and burying method supporting with botulinum toxin injection. The filler was injected in base of the columella, dorsum of the nose and the base of the alar. And then the soft absorbable zigzag lines were buried through micro hole to elevate the dorsum and the columella to raise the tip of the nose. Then the dorsal muscle, dilator muscle of naris, musculus depressor septinasi were relaxed by botulinum toxin. Results All patients were followed up for 1 year, different degrees of improvement were obtained. No infection and nasal septal perforation were observed. The patient's downtime was only 4-5 days. Fifty patients were satisfied with the aesthetic effect in the questionnaire, 6 cases were generally satisfied, the satisfaction rate was 89.3%. Conclusion The comprehensive rhinoplasty of filler injection and burying method supporting with botulinum toxin injection is minimal invasive and effective with short recovery period. It is proved to be worthy of popularization and application. Related Articles | Metrics

Select

Application of Modified Bipolar Radiofrequency-Assisted Liposuction Technique in Body Contouring CAI Lei,HAN Xuefeng,JIN Shaodong,WANG Bingqing,LI Facheng 2017, 13 (2):  93-95.  DOI: 10.3969/j.issn.1673-0364.2017.02.010 Abstract ( 363 )   Objective To explore the clinical efficacy of modified bipolar radiofrequency-assisted liposuction technology for body contouring. Methods From September 2012 to September 2015, 82 patients with skin relaxation were treated with modified bipolar radiofrequency-assisted liposuction. The aspiration was performed using a blunt cannula with low negative pressure firstly, then bipolar radiofrequency-assisted liposuction was used to stimulate profound contraction of the fibroseptal network for enhancing skin contraction. Satisfaction surveys of postoperative patients were conducted, and 3 independent plastic surgeons evaluated contour and skin quality with randomized preoperative and postoperative photographs postoperatively. Results All patients were successfully performed the operation, no serious complication was observed. The patients were followed up for 6 months. All patients were satisfied with their contouring result and degree of skin tightening. Conclusion Modified bipolar radiofrequency-assisted liposuction represents a safe procedure to achieve significant improvement of the skin laxity and fat deposition, and is especially suitable for the patients with skin relaxation. Related Articles | Metrics

Select

Random Skin Flap in Repairing Skin Defects in Maxillofacial Region XIE Youqun,GU Songjie 2017, 13 (2):  96-97.  DOI: 10.3969/j.issn.1673-0364.2017.02.011 Abstract ( 365 )   Objective To explore the use of random skin flap in repairing maxillofacial skin defects after tumor resection. Methods From March 2014 to September 2015, 38 cases received tumor resection in maxillofacial region. According to the area of skin defect and specific situation, the appropriate random skin flap was used instantly to repair the defects. Results All the facial flaps survived with no necrosis and complications. The patients were followed up for 6-24 months, good appearance and function were both achieved. Conclusion Random skin flap is effective in repairing the maxillofacial skin defects after tumor resection with simple and flexible operation, which is worthy of clinical promotion. Related Articles | Metrics

Select

Application of Mini Camera GoPro Hero 4 in Video Capture of Plastic and Reconstructive Surgery Procedures CHEN Chuhsin,LIU Kai 2017, 13 (2):  98-101.  DOI: 10.3969/j.issn.1673-0364.2017.02.012 Abstract ( 974 )   Objective To explore the application of GoPro in recording plastic surgery for documentary and tutorial purposes, thus obtaining optimal parameters for operative shoots. Methods The GoPro HERO 4 Silver Edition camera was head-mounted on the surgeon and oriented to the surgeon's perspective using the GoPro App. The camera was used to record:hemangioma interventional surgery (1 case), nasal reconstruction procedure (1 case), pouch resection (1 case) and rhinoplasty with autologous ear cartilage graft (1 case). During case 3, the surgeon was equipped with an additional headlamp. For case 4 the GoPro was mounted with a macro lens to zoom up close on the surgical field. Results Camera settings for case 1 were as follows: 1080 p video resolution; 60 fps; low-light mode on; narrow field of view; 16:9 aspect ratio. The intervention display screen shown in the footage was overexposed. Within case 2 to case 4, the parameters mentioned above were adjusted to a narrow field of view, which enabled the camera's automatic white balance to better compensate for bright lights focused on the surgical field. The video captured in Case 2 was adequate for teaching or presenting. During case 3 the lighting contrast resulted in limited exposure due to the headlamp. For case 4, macro photography captured more details. Conclusion The GoPro HERO 4 Silver Edition camera enables high-quality, cost-effective video recording of plastic and reconstructive operation. When set to a narrow field of view and automatic white balance, the camera is able to sufficiently compensate for the contrasting light environment of the operating room and capture high-resolution and detailed video. Related Articles | Metrics

Review

Select

Research Progress of Tissue Engineering Dermal Scaffold Materials LI Xiaobing 2017, 13 (2):  102-115.  DOI: 10.3969/j.issn.1673-0364.2017.02.013 Abstract ( 325 )   The skin tissue engineering has developed rapidly. The dermal scaffold plays a key role in the construction of tissue engineered skin. In this paper, the latest research status in the field of the physical and chemical properties, biological applicability of 3 kinds of dermal scaffold materials were reviewed. The advantages and problems of various kinds of scaffold materials were analyzed. The future development direction was put forward. Related Articles | Metrics

Select

Research Advances of Neovascularization in Tissue Engineering Skin WANG Chuan 2017, 13 (2):  106-108.  DOI: 10.3969/j.issn.1673-0364.2017.02.014 Abstract ( 462 )   Blood supply is the most important thing regarding skin's maintenance and transplantation, although tissue engineering skin have been commercialized and used in clinic, it has failed to achieve the vascularization. The neovascularization of tissue engineering skin at present is mainly rely on seed cells that can promote vascularization and a variety of growth factors. As the development of induced pluripotent stem cells, electrostatic spinning, and the biological printing technology, new scaffolds combined with the corresponding seed cells and cytokines can be designed to simulate the growing environment of body accurately to promote vascularization of tissue engineering skin. In this paper, the research progress of neovascularization in tissue engineering skin was reviewed. Related Articles | Metrics

Select

Regenerative Microenvironment and Cicatrization after Peripheral Nerve Injury YE Hualong 2017, 13 (2):  109-112.  DOI: 10.3969/j.issn.1673-0364.2017.02.015 Abstract ( 509 )   Peripheral nerve injury is very common in clinic. Different from the central nervous system, peripheral nerve has a certain regenerative capacity. In this paper, the changes of the regeneration microenvironment and scarring associated factors after peripheral nerve injury were all reviewed. Related Articles | Metrics

Select

Research Progress and Clinical Application of Concentrated Growth Factors in Plastic and Reconstructive Surgery CHEN Xia,WANG Jian 2017, 13 (2):  113-115.  DOI: 10.3969/j.issn.1673-0364.2017.02.016 Abstract ( 341 )   Concentrated growth factors (CGF) are the third-generation autologous platelet concentrates, which contain multiple of growth factors and fibrin matrix. In this paper, the application efficacy and safety of CGF in plastic and reconstructive surgery were reviewed. Related Articles | Metrics

Select

The Clinical Use of Gracilis Muscle Flap in Plastic Surgery WANG Keming 2017, 13 (2):  116-117.  DOI: 10.3969/j.issn.1673-0364.2017.02.017 Abstract ( 615 )   Gracilis is located in the medial compartment of the thigh. The main pedicle of this muscle arises from the medial circumflex femoral vessels and profunda femoris vessels. The gracilis receives its innervation from the anterior division of obturator nerve. Based on the pedicles, the gracilis muscle flap and the perforator flap are widely used in plastic surgery in recent years. In this paper, the clinical use of the gracilis flap and perforator flap in plastic surgery were reviewed. Related Articles | Metrics

Select

755 nm Alexandrite Laser in the Treatment of Port-Wine Stain YU Wenxin,MA Gang,ZHU Jiafang,WANG Tianyou,CHENG Yijie,LIN Xiaoxi 2017, 13 (2):  118-120.  DOI: 10.3969/j.issn.1673-0364.2017.02.018 Abstract ( 694 )   Pulsed dye laser (pulsed dye laser, PDL) is still the gold standard to the treatment of port-wine stain (PWS), however, few PWS are lightened completely with PDL. PDL-resistant PWS exist in cutaneous laser practice and constitute a difficult management problem. Nowadays, factors that have resulted in the high degree of therapeutic recalcitrance are identified, and some experimental strategies are addressed, such as: photodynamic therapy, the laser application with deeply penetration, angiogenesis inhibitors, etc. The 755 nm alexandrite laser, compared to 595 nm pulsed dye laser, have deeper penetrating of organization, less melanin absorption, higher laser energy. In this paper, the 755 nm alexandrite laser for the treatment of port-wine stains was reviewed. Related Articles | Metrics