Table of Content

23 July 2020, Volume 12 Issue 1 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Original article

Select

The Experiment of Keratinocyte Growth Factor Bioactive Peptides＇ Synthesis and Promoting Epidermal Cell Proliferation ZONG Xianlei,LU Haibin,QI Zuoliang,LI Guoju,SONG Guodong,DU Le,JIN Xiaolei,JIANG Duyin 2016, 12 (1):  1-5.  DOI: 10.3969/j.issn.1673-0364.2016.01.001 Abstract ( 355 )   Objective To isolate sequences from a phage display 7-mer peptide library, synthesize keratinocyte growth factor（KGF） bioactive peptide from the isolated sequences and to evaluate the effects of KGF bioactive peptide on epidermal cell proliferation. Methods Using monoclonal anti-human KGF antibody as the target, a phage display 7-mer peptide library was screened and target sequences were isolated. The sequences were then modified to generate KGF bioactive peptides. Immunofluorescence assay was performed to evaluate the binding activity of KGF peptides on epidermal cells.CCK-8 was used to detect the effects of KGF bioactive peptide on epidermal cell proliferation. RT-PCT and Western-blot were used to detect the expression of KGF receptor of epithelial cells. Results Two peptides similar to KGF was isolated from a phage display 7-mer peptide library and then modified to generate two KGF bioactive peptides. KGF bioactive peptides were then purified to reach a purity of 98%. The C terminal of KGF bioactive peptides was closed, and the N terminal was labeled with rhodamine dye laser. KGF bioactive peptides were able to combine with the epidermal cells showed by Immunofluorescence assay. CCK-8 test results showed that compared with the control group, the two KGF bioactive peptides promoted epidermal cell proliferation in a dose-dependent manner. RT-PCR and Western-blot test results showed two KGF bioactive peptidegroup enhanced the expression of KGFR on epidermal cells. Conclusion KGF bioactive peptides can be synthesized based on sequences screened from phage display peptide library. KGF bioactive peptides are capable of combining with KFGR, enhancing the expression KGFR, and thereby promoting the proliferation of epidermal cells. The results are expected to help promoting wound healing. Related Articles | Metrics

Select

The Influence of Stromal Cell-derived Factor-1 Promoting Angiogenesis of Adipose-derived Stem Cells via CXCR4 and CXCR7 Pathway in vitro LI Qiang,ZHANG Linxia,ZHANG Aijun,TAO Changbo,LI Xueyang,MA Zhibing,JIN Peisheng 2016, 12 (1):  6-10.  DOI: 10.3969/j.issn.1673-0364.2016.01.002 Abstract ( 460 )   Objective To investigate the role of SDF-1 in promoting ADSCs angiogenesis, as well as CXCR4, CXCR7.Methods ADSCs were primary cultured by enzyme digestion method. Flow cytomertry and western blot assays were performed to detect the expression of CXCR4, CXCR7. ADSCs were treated with SDF-1 and neutralizing anti-CXCR4 and/or anti-CXCR7 antibody. ELISA was used to detect the secretion of VEGF, HGF, and β-FGF. h UVECs tube formation assay was used to examine the effect of SDF-1 and neutralizing antibody CXCR4, CXCR7 on promoting angiogenesis property of ADSCs. Results ADSCs were successfully isolated and cultured from human liposuction tissue. CXCR4 and CXCR7 expression were significantly decreased during cell culture. When treated with SDF-1, CXCR4 and CXCR7 expression were significantly increased. In addition, SDF-1 pretreating also promoted the secretion of angiogenesis factors VEGF, HGF, β-FGF and increased the ability of tube formation of h UVECs induced by ADSCs. The blocking of CXCR4 and CXCR7 activation also inhibited the promotion of angiogenesis property of ADSCs caused by SDF-1. Conclusion SDF-1 pretreating can up-regulate the expression of CXCR4 and CXCR7 in ADSCs. Furthermore, it also promotes the angiogenesis ability of ADSCs through CXCR4 and CXCR7 pathways. Related Articles | Metrics

Select

Research of Drug Resistance Gene MDR1 in Keloid Fibroblasts CHEN Yahong,QU Miao,WANG Wenbo,LIU Wei,WU Xiaoli 2016, 12 (1):  11-19.  DOI: 10.3969/j.issn.1673-0364.2016.01.003 Abstract ( 379 )   Objective To compare the expression of drug resistance gene mdr1 between keloid fibroblast（KF） and normal dermal fibroblast（NF）. Methods The expression of drug resistance gene mdr1 and its corresponding protein ABCB1 in KF and NF were assessed with real-time PCR, immunohistochemical and flow cytometry. The expression of ABCB1 protein in 30 cases of keloid was analyzed by flow cytometry. Results The expression of mdr1 and ABCB1 in keloid was significantly higher than in normal skin; The expression rate of ABCB1 of the primary fibroblasts in pectoral keloid was higher than in abdominal and dorsal keloid. Conclusion Drug resistance gene mdr1 and its corresponding protein ABCB1 are all expressed in KF, and the expression amount is much higher than in NF. Related Articles | Metrics

Select

Statistical Relationship between Mandibular Deformity and External Ear Malformation in Hemifacial Microsomia Andy Tan,XIN Yu,Melia Bogari,LIN Li,XU Haisong,ZHANG Yan,CHAI Gang 2016, 12 (1):  15-19.  DOI: 10.3969/j.issn.1673-0364.2016.01.004 Abstract ( 351 )   Objective To analyze the statistical correlation between the mandibular deformity and external ear malformation of hemifacial microsomia（HFM）. Methods From January 2013 to December 2014, 118 cases with HFM were enrolled according to the modified OMENS plus classification system. Multiple view angle of photography, facial 3D scanning and head CT scan were performed in all cases. External ear malformation was classified according to multiple view angle of photography and facial 3D scanning. The mandible was measured and classified by 3D reconstruction and Mimics software.Statistical correlation between mandibular deformity and external ear malformation was analyzed by logistic regression model.Results In 118 patients, all patients showed mandibular deformity： type M1 in 27 patients（22.9%）, type M2 A in 45patients（38.1%）, M2 b type in 41 patients（34.7%） and type M3 in 5 patients（4.2%）. All patients presented mild to severe ear malformation： type E1 in 20 cases（16.9%）, type E2 in 24 cases（20.3%）, type E3 in 51 cases（43.2%） and type E4 in 23cases（19.5%）. Correlation coefficient（P =0.647） showed no significant statistical correlation between these two criteria.Conclusion There is no significant correlation of the severity between the mandibular deformity and external ear malformation in the patients with HFM. Related Articles | Metrics

Select

Stability of Operation-first BSSRO Combined Postoperative Orthodontic Treatment for Mandibular Prognathism YANG Liya,LI Shuyuan,TENG Li,SUN Xiaomei,XU Jiajie,LU Jianjian,ZHANG Chao 2016, 12 (1):  20-24.  DOI: 10.3969/j.issn.1673-0364.2016.01.005 Abstract ( 776 )   Objective To explore the stability of operation-first sagittal split ramus osteotomy（SSRO）-postoperative orthodontics therapy for the correction of mandibular prognathism（MP） by cephalometric radiographs. Methods Eighteen patients diagnosed with MP with and without asymmetry were treated. BSSRO-postoperative rapid orthodontics therapy was performed without preoperative orthodontics therapy and lateral cephalometric radiographs were taken before operation（T0）,five days after operation（T1） and one year after operation（T2）. The landmarks were measured by the software. Results The mean surgical setback was 7.5 mm at B point and 6.6 mm at pogonion. The mean long-term horizontal relapse was 2.1 mm（28.0%） at B point and 2.9 mm（43.9%） at pogonion. The mean vertical surgical changes showed downward displacement of B point（1.3 mm） and pogonion（1.0 mm）. The mean long-term vertical relapse was 0.1 mm at B point and 0.3 mm at pogonion.The mean increase was 4.3 ° at ramus angle and the long-term relapse was 1.1 °（25.6%）. The magnitude of the surgical movement of B point and pogonion（T1-T0） didn ＇t correlate with long-term changed distance（T2-T1） horizontally and vertically（P0.05）. The magnitude of the surgical movement of Go, Condylion point and ramus angle（T1-T0） didn ＇t correlate with long-term changed distance of Pog point（T2-T1） horizontally（P 0.05）. Conclusion The skeletal relapse do exist in the BSSRO-postoperative rapid orthodontics therapy for MP and there is no linear correlation between the surgical movement of B point, pogonion and skeletal relapse. Related Articles | Metrics

Select

The Different Reconstructive Methods for Scalp Defect MA Jie,SHEN Zunli,SHEN Hua,ZHANG Zhaofeng 2016, 12 (1):  25-30.  DOI: 10.3969/j.issn.1673-0364.2016.01.006 Abstract ( 540 )   Objective To explore the different reconstructive methods for scalp defect. Methods From June 2007 to June 2014, 36 patients with scalp and calvaria defects caused by trauma（16 cases） and tumor（20 cases） were treated with direct suturing or local flap（28 cases）, fascial flap combing skin grafting（4 cases）, remote skin flap（2 cases） and free flap（2cases）. Results All the patients were followed up for 1 to 6 months, the flaps including skin grafts were all survived, and no major complications were observed. In the patients treated with direct suturing or local flap, the scar was less obvious and the hair grew better compared with other methods. Conclusion Local flap is an excellent reconstructive option for repairing the scalp defect with its convenience, high survival rate, good hair growth and good appearance. Related Articles | Metrics

Select

Surgical Management of Giant Neurofibroma in Soft Tissue WANG Jin,YUAN Siming,HONG Zhijian,CUI Lei,GUO Yao,WANG Jun,HU Xinbao,JIANG Huiqing 2016, 12 (1):  27-30.  DOI: 10.3969/j.issn.1673-0364.2016.01.007 Abstract ( 450 )   Objective To explore the effects of surgical management for the treatment of giant neurofibroma in soft tissue.Methods From January 2004 to December 2013, 21 patients with giant neurofibromas were treated surgically. According to the location and extent of the lesion, as well as the adjacent anatomy, surgical management was performed： partially or almost completely excision with surgical flap or skin graft for repairing. Expander and preoperative interventional embolization of tumor＇s nutrient arteries were used when appropriately. Results In this study, partially（n=10） or almost completely（n=4）removal of the tumor was performed. The wounds were repaired by skin flap or skin graft. One case underwent expander treatment and 6 cases underwent preoperative interventional embolization of tumor＇s nutrient arteries, which successfully reduced the bleeding in operation. Most of the skin flap and skin graft survived well（17/21）, poor wound healing was observed in 4 cases which healed by dressing. After operation, the local appearance and the function of the limbs were improved obviously. Conclusion For the giant neurofibroma, surgical treatment effectively reduces the tumor burden,rehabilitates the appearance and function, and so improves the quality of life. Skin expandor and interventional embolization of nutrient artery can be used when appropriately. Related Articles | Metrics

Select

Clinical Analysis of Lipo-abdominoplasty： A Report of 35 Cases CHENG Lin, HAN Xuefeng, LI Facheng 2016, 12 (1):  31-33.  DOI: 10.3969/j.issn.1673-0364.2016.01.008 Abstract ( 480 )   Objective To explore the efficacy and safety of lipo-abdominoplasty in remodeling abdominal contour.Methods From June 2011 to February 2015, 35 patients underwent lipo-abdominoplasty. Liposuction was performed on the flanks and whole abdomen using superwet technique with a blunt cannula of 3 mm or 2.5 mm diameter. After liposuction,selective subcutaneous separation was performed to reshape abdominal contour. The undermining area was limited to the internal borders of the rectus abdominal muscles. Following removal of the redundant skin and subcutaneous tissue, the abdominal flap was sutured using the progressive tension anchor suture technique. Results Primary wound healing was achieved in 28 patients. There was hematoma in 3 cases, partial wound dehiscence in 3 case, which improved after symptomatic treatment. No flap necrosis occurred. After followed up for 3 to 26 months, the abdomens were flexible, and the incision scar was not invisible. All the patients were satisfied with abdominal contour. Conclusion Lipo-abdominoplasty is a safe and effective technique for abdominal contouring. Related Articles | Metrics

Select

Application of Autologous Fat Graft in Treating Facial Localized Scleroderma WANG Yang,CHEN Bo,WANG XiaoJun 2016, 12 (1):  34-36.  DOI: 10.3969/j.issn.1673-0364.2016.01.009 Abstract ( 494 )   Objective To explore the clinical efficacy of integrated technique of autologous fat graft in treating facial localized scleroderma. Methods From January 2009 to August 2015, 12 patients with facial localized scleroderma were treated by autologous fat graft（3-4 times, six months apart）, combining with V-Y plasty and adhesion release. Results All the patients were followed up for 8 to 18 months and were satisfied with the high survival rate of fat, facial morphology and color improvement. No complications like hematoma, subcutaneous induration and infection were observed. Conclusion Integrated technique of autologous fat graft is a safe and effective method for facial localized scleroderma, and is worthy of clinical promotion. Related Articles | Metrics

Select

Application of Portable 3D Scanner in Quantitative Assessment of Scar in Clinic SHEN Danfeng,SHI Yuan,XIA Lingling,GAO Zhen,WU Xiaoli,LIU Wei 2016, 12 (1):  37-40.  DOI: 10.3969/j.issn.1673-0364.2016.01.010 Abstract ( 457 )   Objective To explore the potential and feasibility of portable 3D scanner in quantitative assessment of scar in clinic. Methods From April 2015 to November 2015, 10 patients were randomly selected for scar scanning with portable3 D scanner of high resolution. The scanned data were analyzed with specific software for scar quantitative characterization.Correlation and Difference between scanned data and manually measured data were also analyzed. Results The scanned data were significantly correlated with the manually measured data, and the difference was also significant（P 0.05）.Conclusion The portable 3D scanner with high solution can reliably reveal scar characters via 3D image reconstruction and quantitative analysis, and therefore is possible to become an effective tool for objective and quantitative analysis, and to be applied for scar diagnosis and assessment of clinical therapy. Related Articles | Metrics

Select

The Application of Surgical Excision Combining Shallow X-ray Radiation in the Treatment of Keloids WANG Weijia,XIE Yixin,YANG Bin,HE Renliang,XI Wenjing,ZHANG Yixin 2016, 12 (1):  41-62.  DOI: 10.3969/j.issn.1673-0364.2016.01.011 Abstract ( 693 )   Objective To observe the clinical effects of surgical excision combining shallow X-ray radiation in the treatment of keloids. Methods Two hundreds cases with keloids were enrolled, including 166 cases with the diameter less than 10 cm（83%） and 34 cases with the diameter more than 10 cm（17%）. In 132 cases, the duration was less than 5 years（66%）, while in 68 cases, the duration was more than 5 years（34%）. 121 cases received X-ray radiation immediately after surgery（60.5%）, 58 cases after clearing（29%） and 21 cases after incision healed（10.5%）. All the patients were followed up for 2 years after radiotherapy, and the clinical effects were observed. Results The keloids recurred in 31 cases within 2years（15.5%）, the effective rate was more than 80%. The effective rate of radiotherapy immediately after surgery, after clearing and after incision healed were 97%, 90% and 58% respectively. The recurrence rate was 6.63% in the keloids with the diameter less than 10 cm, and 23.5% in the keloids with the diameter more than 10 cm. The recurrence rate was 8.3% in the patients with the duration less than 5 years, and 11.7% in the patients with the duration more than 5 years. Radiationinduced ulceration was observed only in one case, and no other severe complication was observed. Conclusion Surgical excision combining shallow X-ray radiation is a safe and effective method for the treatment of keloids. Early postoperative radiotherapy can reduce the recurrence rate. The effect in the patients with less diameter and shorter duration is more obvious. Related Articles | Metrics

Review

Select

Notch Signaling in Chondrogenic Development and Differentiation LU Xiaona,FAN Fei 2016, 12 (1):  44-47.  DOI: 10.3969/j.issn.1673-0364.2016.01.012 Abstract ( 439 )   Notch signaling widely exists in many kinds of animal cells, and it is important to regulate cell proliferation,survival, differentiation and apoptosis. As both its receptor and ligand are trans-membrane proteins, Notch signaling is significant in mediating communication between cells. The chondrogenic development and differentiation start from the agglutination of ectomesenchymal cells. Notch family expresses diversely in the formation of cartilages, regulating them depending on time and space. In this paper, the composition, distribution and transduction mechanisms of Notch signaling and its effects on chondrogenic development and differentiation were reviewed. Related Articles | Metrics

Select

Application of Graphene-based Materials in Tissue Engineering ZOU Yaguang,WEI Jiao,LI Qingfeng 2016, 12 (1):  48-51.  DOI: 10.3969/j.issn.1673-0364.2016.01.013 Abstract ( 287 )   Graphene is a kind of atomic crystal nanomaterial arranged in a two-dimensional structure with extraordinary physicochemical properties, which have been extensively employed in various fields. However the application of graphene in regenerative medicine is still in an early phase. In this paper, the study of toxicity of graphene-based materials and its ability of inducing stem cell differentiation into osteogenic, neuronal, cardiomyogenic and adipogenic lineages were reviewed. Related Articles | Metrics

Select

Research Progress of Clinical Therapy for Lymphedema DENG Jingcheng,DAI Tingting,CAO Weigang 2016, 12 (1):  52-55.  DOI: 10.3969/j.issn.1673-0364.2016.01.014 Abstract ( 618 )   Lymphedema is not rarely seen clinically, and the most common etiology for the forming of lymphedema is the impaired flow of lymph fluid through the draining lymphatic vessels or lymph nodes. If the uninjured lymphatic vessels are unable to accommodate the increased lymphatic load, an abnormal accumulation of protein-rich lymph fluid develops in the tissues spaces. Lymphedema, without intervention, can lead to progressive swelling, fibrosis of the soft tissues, pain,paresthesias and infection. Therefore, early identification of the signs and symptoms of lymphedema is the key factor of the patients＇ prognosis, and complications can be minimized by early treatment. In this paper, progress of treatment in lymphedema was reviewed. Related Articles | Metrics

Select

The Research Advance of Lipo-abdominoplasty PENG Li,ZHANG Jie 2016, 12 (1):  56-59.  DOI: 10.3969/j.issn.1673-0364.2016.01.015 Abstract ( 359 )   Abdominoplasty is divided into many types according to individual differences. Lipo-abdominoplasty,combined liposuction and abdominoplasty, is widely used at present. By applying the tumescent technique, suction casing and paraumbilical high tension suture and other techniques, the skin necrosis, hematoma and other complications are greatly reduced. In this paper, the reserch progress of lipo-abdominoplasty was reviewed. Related Articles | Metrics

Select

The Causes, Precautions and Treatments of Complication in Autologous Fat Transplantation ZHANG Yi,QIU Xiaodong 2016, 12 (1):  60-62.  DOI: 10.3969/j.issn.1673-0364.2016.01.016 Abstract ( 369 )   Autologous fat transplantation is effective in improving body deformity, partial shaping and facial aging, but there are still risks. Many complications such as blindness, stroke and skin necrosis caused by fat necrosis, oil cysts,calcification and infection caused by fat necrosis, these complications require great attention. In this paper, the causes,preventions and treatments of the complication in autologous fat transplantation were reviewed. Related Articles | Metrics