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Table of Content

    23 July 2020, Volume 11 Issue 5 Previous Issue    Next Issue
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    Original article
    Morphology and Three-dimensional Structure Characteristics of Goat Spinal Dural
    YU Fengbin, CEN Lian, LIAO Xinyuan, CHEN Deyu
    2015, 11 (5):  289-291.  DOI: 10.3969/j.issn.1673-0364.2015.05.001
    Abstract ( 382 )  
    Objective To obtain the morphology and three-dimensional structure characteristics of goat spinal dural and provide information for reconstructing artificial spinal dural. Methods The specimens of spinal dural were obtained to observe the microstructure, surface morphology, histology, collagen fiber diameter, distribution and distribution model through naked eyes, light microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). Results The spinal dura looked like a milky white, translucent, flexible and elastic membrane. It was mainly composed of oriented collagen fibers while fibroblasts line sparsely along those fibers. Its inner and outer surfaces looked like mountain peaks through SEM observation. It was revealed by TEM that collagen fibers distribute orderly with an average diameter of (627±60) nm. The diameters of collagen fibers exhibited a bimodal distribution. Conclusion The goat spinal dura is a translucent membrane which is mainly composed of nanoscale oriented collagen fibers and sparsely scattered fibroblasts.
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    Clinical Effect of Transplantation of Haploid Hematopoietic Stem Cells Combined with Cord Mesenchymal Stem Cells in Treating Aplastic Anemia
    XU Lixin, YAN Bei, ZHANG Xuhui, WU Yamei, LI Xiaohong, CAO Yongbin, WU Xiaoxiong
    2015, 11 (5):  292-294.  DOI: 10.3969/j.issn.1673-0364.2015.05.002
    Abstract ( 451 )  
    Objective To investigate the clinical effect of transplantation of haploid hematopoietic stem cells combined with cord mesenchymal stem cells in the treatment of aplastic anemia. Methods From December 2012 to December 2014, 5 cases with aplastic anemia were treated with haploid hematopoietic stem cells combined with cord mesenchymal stem cells transplantation. The MNC, CD34+cells, GVHD incidence, blood recovery time, changes of related growth factors (like SDF-1α, G-CSF, IL-6, GM-CSF, TPO, SCF), the improvement of bone marrow nucleated cells protein phosphorylation ERK1/2 and complications were all observed. Results Five patients obtained hematopoietic reconstitution with MNC of 11.27 ×108 cells/Kg and CD34+ of 4.0 ×106 cells/Kg. The average platelet recovery time was 15 days and the average recovery time of blood cells was 10.4 days. aGVHD of stage III was observed in 3 patients, localized cGVHD was observed in 3 patients, and they were all improved after treatment. Four patients recovered well, one patient died of fungal pneumonia 73 days after treatment. After transplantation, serum SDF-1α, G-CSF, IL-6, GM-CSF, TPO and SCF were significantly improved (P〈0.05), the expression of phosphorylation ERK1/2 protein of bone marrow nucleated cells was significantly increased ( P〈0.05). Onenbsp;case of EB viremia, two cases of hemorrhagic cystitis and two cases of fungal pneumonia were observed. Conclusion Haploid hematopoietic stem cells combined with cord mesenchymal stem cell transplantation is effective in the treatment of aplastic anemia, it can significantly improve the bone marrow microenvironment of patients with higher safety and lower complication. It is worthy of further clinical application.
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    The Influen ce of Reaction Time on the Biocompatibility of Polydopamine/Silver Nanoparticles Modified Porous Titanium
    RONG Xicang, ZHANG Yu, TAN GnoXin, TAN Ying, NING Chengyun, LI Mei, LI Lihua
    2015, 11 (5):  295-319,326.  DOI: 10.3969/j.issn.1673-0364.2015.05.003
    Abstract ( 374 )  
    Objective To investigate the reaction time influence on the antibacterial property and cytocompatibility of polydopamine/silver nanoparticles modified porous titanium. Methods A bioinspired polydopamine (PDA) layer was deposited on titanium surface by polymerization. By adjusting the response time of nanosize silver particles (15 min, 30 min, 60 min) of the modified titanium, different concentration of silver nanoparticles modified titanium coated by polydopamine were prepared. Using scanning electron microscopy (SEM), cell adhesion, cell toxicity and sterilization rate examination to evaluate the in vitro biocompatibility and antibacterial properties of polydopamine modified titanium with load of different concentration of silver nanoparticles. Results The modified titanium surface material with the response time of 15 min in nanosize silver particles loading, showed better cell adhesion ability, lower cytotoxicity and more satisfactory antibacterial properties than the others. Conclusion By adjusting the reaction time of nano silver deposition, the silver ion release quantity is adjustable, and the polydopamine/silver nanoparticles modified porous titanium, which have good cell compatibility and strong antibacterial ability, can be gained.
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    Biocompatibility and Biosafety Research of New Compound of Fibrin Glue and Calcium Phosphate Bone Cement
    DING Chao, LI Qi, LIN Lijun, SUN Yongjian
    2015, 11 (5):  301-304.  DOI: 10.3969/j.issn.1673-0364.2015.05.004
    Abstract ( 433 )  
    Objective To explore the biocompatibility and biosafety of new compound of fibrin glue and calcium phosphate bone cement, and to investigate the clinical feasibility for bone reconstruction. Methods The new compound of fibrin glue and calcium phosphate bone cement was prepared and the extract liquid was obtained. The acute toxicity test, hemolysis test, micronucleus test, the cytotoxic test were used for the biocompatibility and biosafety evaluation of the new compound. Results The CPBC extract liquid did not cause acute toxicity in mice; No hemolytic reaction was observed in experimental groups by visual inspection and the hemolytic rate was less than 5%; Micronucleus test showed no significant differences between experimental group and negative control group; The extract liquid had no significant impact on growth and differentiation of mice MC3T3 osteoblasts, and the cell toxicity classification was class I. Conclusion The new compound of fibrin glue and calcium phosphate bone cement have good biocompatibility and biosafety.
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    Summary of Clinical and Pathology Characteristics of Giant Congenital Melanocytic Nevi
    WU Min, XIE Feng, YU Qingxiong, LI Qingfeng
    2015, 11 (5):  305-309.  DOI: 10.3969/j.issn.1673-0364.2015.05.005
    Abstract ( 588 )  
    Objective To explore the clinical features, histopathologic manifestations and treatment methods of giant congenital melanocytic nevi (GCMN). Methods The clinic data of 20 GCMN patients were collected and analyzed. The biopsy specimens were obtained for HE, Masson and immunohistochemical (HMB-45, Melan-A, PCNA) staining and were observed under light microscopy. Results All of these cases were sporadic cases and patients were all born with GCMN. The most common lesions were head and face, hindquarters cadres and arms. Typical lesions for GCMN were large-area lesions, with color of light brown to dark black, and covered with hairs (mostly black thick hair). Histologically, GCMN contained nevus cells within deep dermis and even in subcutaneous tissue. The phenomenon of “Grenz zone” and “pigment incontinence” could be observed in the superficial layer of dermis. For immunohistochemical staining, the expression of Melan-A and S-100 were positive in the nevus cells of GCMN, and HMB-45 was focal positive. Positive PCNA staining showed high proliferation state of nevus cells. In history observation after non-surgical treatment, a lot of nevus cells in the superficial layer of dermis were killed while deep nevus cells uninvolved after chemical peeling. After laser treatment, the dermal papilla layer pigmentation disappeared, so the pigment appearance could be partly improved. Conclusion Nevus cells infiltrate deeper in GCMN. Nevus cells in GCMN and melanin cells homologous may have same ancestor and are in highly proliferation state. Chemical peeling treatment and laser therapy, which are regarded as superficial and conservative treatments, both have their unique characteristics. While all of them can only partly improve local appearance and failed to cure. In GCMN which involves large area of body, treatment is still difficult, and new strategies still need to be explored.
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    Manual Lymph Drainage for the Treatment of Upper Limb Lymphedema after Breast Cancer Treatment
    CHEN Jiajia, WANG Li, YU Ziyou, HAN Linghua, LUO Yi, LIU Ningfei
    2015, 11 (5):  310-312.  DOI: 10.3969/j.issn.1673-0364.2015.05.006
    Abstract ( 668 )  
    Objective To explore the effectiveness of manual lymph drainage (MLD) in the treatment of breast-cancer-related lymphedema. Methods From 2008 to 2012, 101 patients with unilateral arm lymphedema after mastectomy were enrolled and divided into three groups based on the circumferential difference (d) between lymphedematous and contralateral limbs prior to MLD:group A (d〈1-2 cm), group B (5 cm≥d≥2 cm), group C (d〉5 cm). After 1 course (15 days) of complex decongestive therapy (CDT):MLD+low elastic bandage, changes of limb circumference and tissue edema were measured and the differences between edema and non-edema arms after treatment were compared to evaluate edema reduction. Subjective feelings of each patient were recorded. Tissue edema were measured with multiple-frequency bioelectrical impedance analysis. Results After CDT treatment, significant reduction in circumference of the edematous limbs ( P〈0.01) and edema fluid in tissue (P〈0.01) were observed. Comparison of the reduction of excess tissue edema and limb circumference among 3 groups showed: group C〉group B〉group A (P〈0.01). All patients were satisfied with the treatment. Conclusion Complex decongestive therapy is effective in treating lymphedema and improving physical appearance of the limb. The patients with more initial circumferential difference get better results in MLD.
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    Clinical Research of the Pectoralis Major Myocutaneous Flap Transfer in the Prevention of Breast Cancer-related Lymphedema after Modified Radical Mastectomy
    WANG Ying, CHEN Fei, SUN Heqing, LU Xuan
    2015, 11 (5):  313-315.  DOI: 10.3969/j.issn.1673-0364.2015.05.007
    Abstract ( 383 )  
    Objective To investigate the clinical effect of Pectoralis major myocutaneous flap transfer on the prevention of breast cancer-related lymphedema after modified radical mastectomy. Methods From September 2013 to June 2014, 68 patients with breast cancer were included and divided into 2 groups, interventional group and control group. The patients in interventional group were treated with modified radical mastectomy plus Pectoralis major myocutaneous flap transfer, while the patients in control group were just received mastectomy. All the patients were followed and the upper extremity lymphedema was evaluated 0.5, 1, 3, 6, 9 months after surgery. Results According to the follow-up results, the incidence of lymphedema in interventional group was obviously lower than in control group, the difference was significant (P〈0.05). Conclusion The Pectoralis major myocutaneous flap transfer after modified radical mastectomy can significantly reduce the chances of upper limb lymphedema, and accelerate the postoperative recovery.
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    Clinical Observation on the Effect of Skin Wound Tension Reduction Device on Inhibiting Post-surgery Scar
    CHEN Libin, CHEN Yahon, GAO Zhen, ZHAO Li, HE Chenguang, WU Xiaoli
    2015, 11 (5):  316-319.  DOI: 10.3969/j.issn.1673-0364.2015.05.008
    Abstract ( 729 )  
    Objective To explore the effect of the skin tension reduction device on reducing scar formation after excisional surgery of hypertrophic scars and keloids. Methods Total 10 patients with hypertrophic scars and keloids were enrolled in this study. All of them received excisional surgery. Total 10 patients were involved as a self control study with part of wound treated with tendon reduction device, the other part with no treatment as control. All patients were observed for scar formation at surgical wound sites for 3 months post-surgery. Scar width and Vancouver scar scale were used as the parameter to quantify the differences between two groups. Results Compared with control group, the postoperative scar in tension reduction group was narrower in scar width and lower in the Vancouver scar scale scores with statistical significance (P〈0.05). Conclusion Application of the skin reduction device can efficiently inhibit wound tension induced scar formation at post-surgery sites with significant reduction of scar width and hyperplasia after excisional surgery of hypertrophic scars/keloids.
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    Biomechanical Research on the Fixation of Fractures by External Locking Plate and External Fixator
    LI Qiao, TAN Jinhong, MA Xiaoming, LIANG Chunxian, LI Diyi
    2015, 11 (5):  320-322.  DOI: 10.3969/j.issn.1673-0364.2015.05.009
    Abstract ( 483 )  
    Objective To explore the biomechanics of the fractures fixation by external locking plate and external fixator, and to provide a theoretical basis for clinical application of external locking plate in fracture fixation. Methods Bovine bones of different lengths were chosen as the artificial fracture specimens and external locking plate fixation and external fixation were adopted respectively to observe the axial compression, torsion and bending changes under different loads by using a variety of testing methods. Results The differences of the axial compressive load values between the two groups under 100, 200, 300, 400, 500, 600 and 700 N were statistically significant (P〈0.05), with the larger of the load, the greater of the gap of axial compressive values. The differences of torsion angles between the two groups under 4, 6, 8, 10, 12, 14 N.cm were statistically significant (P〈0.05), and the greater of the load, the bigger of the gap of torsion angles. The differences of bending load displacement values between the two groups under 50, 100, 150, 200, 250, 300 N were statistically significant (P〈0.05), and with the greater of the load, the larger of the gap in bending displacement values. Conclusion The biomechanics of external locking plate fixation is better than the external fixator, and it can be clinically used as external fixators, providing a new method for the clinical treatment of open fractures.
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    Beak Lock Ligament Reconstruction with Double Endobutton Plate and Suture-anchor in the Treatment of TossyⅢ Dislocation of Acromioclavicular Joint
    XU Jun, JIANG Xuefeng, LIU Shanglou, SUN Huiqing, ZHOU Xiaojian
    2015, 11 (5):  323-326.  DOI: 10.3969/j.issn.1673-0364.2015.05.010
    Abstract ( 420 )  
    Objective To explore the outcomes of double Endobutton plate and suture-anchor for beak lock ligament reconstruction in the treatment of Tossy Ⅲ dislocation of acromioclavicular joint. Methods From February 2009 to August 2010, 30 patients with Tossy Ⅲ dislocation of acromioclavicular joint were treated with only Double Endobutton (group A), while 28 patients with Tossy Ⅲdislocation of acromioclavicular joint were treated with double Endobutton plate and suture-anchor from March 2011 to May 2012 (group B). The postoperative effect of the shoulder were evaluated and compared between the two groups according to the Karlsson scoring system. Results In group A, the vertical distance from shoulder peak to clavicle side of 3 cases was more than 10 mm, the acromioclavicular joint gap width of 3 cases was more than 10 mm, while no case was observed in group B (P〈0.05). There was no significant difference in strength and shoulder joint activity between the two groups (P〉0.05). But the number of cases in pain of group A were more than group B (P〈0.05). Conclusion Double Endobutton plate and suture-anchor for the treatment of Tossy III dislocation of acromioclavicular joint can lead to more stable outcomes and relieve postoperative pain symptoms.
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    Comparison of the Asian Proximal Femoral Intramedullary Nail Anti-rotation (PFNA II) with and without Using Guide Wire and Reaming for the Treatment of Elderly Osteoporosis Intertrochanteric Fractures
    DOU Lianrong, DOU Bang, ZHU Wei, MA WenQian, LU Mioo, QIN Too
    2015, 11 (5):  327-330.  DOI: 10.3969/j.issn.1673-0364.2015.05.011
    Abstract ( 428 )  
    Objective To compare the outcomes of Asian proximal femoral intramedullary nail anti-rotation (PFNAⅡ) with and without using guide wire and reaming for the treatment of elderly osteoporosis intertrochanteric fractures. Methods From January 2010 to March 2014, 60 elderly patients with osteoporosis intertrochanteirc fractures were treated with PFNA Ⅱ. They were randomly divided into group A and group B (n=30). In group A, guide wire and reaming were used before inserting the nail, while not used in group B. The operation time, intraoperative blood loss, pre-and post-operative hemoglobin, X-ray exposure times and postoperative outcomes between the two groups were compared. Results The average operation time of group A was 50.5 min (40.5-80 min), average intraoperative blood loss was 75 mL (45-120 mL), average pre-operative hemoglobin was 95 g/L (84-120 g/L), and 85 g/L (55-115 g/L) post-operative. 2 patients accepted concentrated red blood cells transfusion of 2 units for blood loss. The average operation time of group B was 39 min (30-50 min), average intraoperative blood loss was 35 ml (30-55 mL) with no post-operative blood transfusion, average pre-operative hemoglobin was 95.3 g/L (80-125 g/L), and 85 g/L (75-120 g/L) post-operation. No patients received blood transfusion. X-ray exposure times in group B was 3 times less than in group A for each patient on average. All the patients were followed up for 3-12 months (mean, 9 months). The Harris hip score was 84.5 (70-93) in group A and 89.5 (80.5-95) in group B. No varus hip deformities, screw cutouts, femoral shaft fractures or failure of internal fixation were observed. Conclusion When PFNAⅡis used to treat osteoporosis interonchanteric fractures of elderly patients, comparing with using guide wire and reaming, not using guide wire and reaming has the advantages of shorter operation time, less intraoperative and post-operative blood loss, and better results.
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    Review
    Vascularization Strategy of Tissue Engineered Bone
    WU Xiaowei, WANG Qian, CAO Yilin, XIAO Ran
    2015, 11 (5):  331-334.  DOI: 10.3969/j.issn.1673-0364.2015.05.012
    Abstract ( 437 )  
    The development of bone tissue engineering provides a promising way to repair large segment of bone defects. And it has already showed tremendous potential for application on the basis of numerous animal experiments and rising clinic studies. However, osteogenesis of the tissue engineered bone (TEB) grafts may be unstable after TEB constructs implanted in vivo, especially in sites with poor blood supply. Recent studies have demonstrated that the vascularization of TEB plays a vital role in generating large segment of bone grafts. In this article, the advances of TEB vascularization strategy were reviewed.
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    Research Advance in Mechanism of Keloids' Invasive Growth
    CHEN Yahong, WU Xiaoli
    2015, 11 (5):  335-338.  DOI: 10.3969/j.issn.1673-0364.2015.05.013
    Abstract ( 434 )  
    Keloid is characterised by an abnormal deposition of extracellular matrix components (collagen in particular) unique to human being, which is usually triggered by skin trauma. It. Keloid is similar to benign tumor in its biological behaviors such as aggressive growth beyond the original boundary of skin injury and invasion into normal tissue. Keloid rarely regresses, recurs at a high rate after therapies such as surgical excision, intralesional steroid injection, chemotherapy and radiotherapy. In this paper, the updated findings of molecular of keloids' invasive growth were reviewed.
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    Research Progress on the Maxillary Development in Patients with Cleft Lip and Palate
    JIANG Chanyuan, YIN Ningbei
    2015, 11 (5):  339-342.  DOI: 10.3969/j.issn.1673-0364.2015.05.014
    Abstract ( 495 )  
    The patients with cleft lip and palate have different degrees of maxillary retrusion. Intrinsic developmental deficiency as well as functional and iatrogenic factors often result in the inhibition of maxillary growth. In this paper, the morphology of cranial base and the maxillary complex, the relative position of the maxilla to the cranial base, and the reasons leading to the maxillary retrusion were reviewed.
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    Research Progress of Lymphaticovenular Bypass in the Treatment of Breast Cancer Related Lymphedema
    ZHA NG Xue, CHEN Ru, CAO Yilin, MU Lanhua
    2015, 11 (5):  343-345.  DOI: 10.3969/j.issn.1673-0364.2015.05.015
    Abstract ( 395 )  
    Lymphaticovenular bypass is a physiological treatment for the upper extremity lymphedema in breast cancer patients. In this paper, the evolution and theoretical basis of lymphaticovenular bypass were introduced, its novel improvements and expectations in three critical aspects were discussed, including evaluation of lymphedema, anastomosis and location.
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    The Application of Fillers in Cosmetic Injections
    QIU Jia'en, LI Qingfeng
    2015, 11 (5):  346-349.  DOI: 10.3969/j.issn.1673-0364.2015.05.016
    Abstract ( 324 )  
    Cosmetic injections have a history of over one hundred years. Nowadays, many new fillers such as hyaluronic acid are widely used. With the development of science and technology, fillers and injection technologies are undergoing rapid improvement. In this paper, various soft tissue fillers and related study progress were reviewed.
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