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23 July 2020, Volume 10 Issue 2 Previous Issue    Next Issue

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Electrospun Nanofibrous Gelatin/Polycaprolactone Membrane for Epidermis Engineering WU Wei,DUAN Huichuan,ZHANG Wenjie,CAO Yilin 2014, 10 (2):  61-68.  DOI: 10.3969/j.issn.1673-0364.2014.02.001 Abstract ( 304 )   Objective To investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun nanofibrous membrane for epidermis engineering. Methods The biocompatibility of the membrane was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membrane were determined before and after seeding HaCaT cells. Cell proliferation assay was tested by CCK-8 kit. Then the outcome of GT/PCL membranes repairing skin defects in the nude mouse was investigated. Results The cell proliferation assay showed good attachment and proliferation of HaCaT cells on the membranes and demonstrated good biocompatibility of the membranes. The membranes were strong enough to be transplanted confirming by mechanical test. Further in vivo transplantation studies revealed that engineered epidermis with GT/PCL membranes are able to repair skin defects of the nude mouse. Conclusion The modified GT/PCL electronspun nanofibrous membrane is a kind of suitable scaffold for epidermis engineering. Related Articles | Metrics

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Study of Constructing Tissue Engineered Urethra Membrane Using Hair Follicle Stem Cells and Nano Silk Fiber in Vitro WANG Ping,DU Mingjun,XIE Haibo,FU Qiang 2014, 10 (2):  69-72.  DOI: 10.3969/j.issn.1673-0364.2014.02.002 Abstract ( 261 )   Objective To explore the feasibility of constructing tissue engineered urethra membrane using hair follicle stem cells and nano silk fiber. Methods Hair follicle stem cells were isolated by enzymatic digestion and fluorescent cell sorting, then cultured in vitro. The maximum amplification factor and colony forming rate were calculated; K19, p63, CD29 positive expression rate were tested by flow cytometry. The hair follicle stem cells were then seeded on electro-spun nano silk fibers to construct tissue engineered urethra. The construction of tissue engineered urethra in vitro was observed by histological observation and fluorescence staining. Results The maximum amplification factor of rabbit hair follicle stem cells and normal hair follicle cells were (5.92±0.77)×104 times and (6.61±3.62)×103 times, and the colony forming rate were (9.07±1.18)%and (3.95±1.01)%respectively. The expression rate of K19, p63, CD29 in hair follicle stem cells were (89.36±6.69)%, (92.10±5.49)%, (89.98±6.89)%, and in normal hair follicle cells were (39.38±2.20)%, (40.78±2.61)%, (40.57±2.79)%. Stratified epithelium-like structure were formed after hair follicle stem cells seeded on the nano silk fibers, and AE1/AE3 staining was positive. Conclusion Rabbit hair follicle stem cells combining with electro-spun nano silk fibers can successfully construct tissue engineered urethral, which can be used for the further study of in vivo repair. Related Articles | Metrics

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The Application of Embryonic Skin Cell in Repairing Skin Defect of Mouse GUO Xiangkai,HAN Zhihua,DUAN Huichuan,QIAN Dejian,XI Linhe,MENG Fei,LIU Ju,WANG Yan 2014, 10 (2):  73-76.  DOI: 10.3969/j.issn.1673-0364.2014.02.003 Abstract ( 301 )   Objective To explore the efficacy of repairing skin defect of mouse by using embryonic skin cell. Methods Animal model of back skin damage in mice was built. The embryonic skin cells were transplanted into the back skin damage of mice as treatment group (Nor-T group, n=6) and the mice in control group (Nor group, n=6) were received no treatment. The wound healing situation were observed 1, 2 and 3 weeks after operation. The expression of TGF-βwas detected 1 and 2 weeks after operation. Results The regenerative skin growth was observed on the wound area in Nor-T group, while only crusta was observed on the wound area in Nor group 1 week after operation. The regenerative skin merged with surrounding skin in Nor-T group, while the wound healed with scar in in Nor group 2 weeks after operation. The hair was observed in Nor-T group, while no hair was observed in Nor group 3 weeks after operation. In Nor-T group, TGF-β1 was expressed 1 week after operation and TGF- β3 was expressed 2 week after operation. Conclusion The embryonic skin cells possess the ability of regenerative skin formation, and TGF-βhas different expression at different wound healing phase. Related Articles | Metrics

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Inhibitory Effects of Ethosomes Encapsulated with 5-Fluorouracil on Human Hypertrophic Scar Fibroblasts ZHANG Zheng,WO Yan,ZHAGN Zhen,MAO Xiaohui,SU Weijie,PU Zheming,ZHANG Yan,ZHANG Yixin 2014, 10 (2):  77-81.  DOI: 10.3969/j.issn.1673-0364.2014.02.004 Abstract ( 335 )   Objective To establish ethosomes encapsulated with 5-Fluorouracil and to explore the inhibitory effects of 5-FU on human hypertrophic scar fibroblasts. Methods Ethosomes encapsulated with 5-Fluorouracil was prepared by Touitou method and was evaluated. Human hypertrophic scar fibroblasts were cultured in vitro, the penetration of the fluorescent probes into fibroblasts was examined by CLSM, and hydroalcoholic solution was set as control group. The effect of ethosomes encapsulating different concentration of 5-FU on fibroblast viability was tested by cell counting kit-8 (CCK-8), and the median inhibitory concentration (IC50) was calculated. The effect of ethosomes on fibroblast viability was tested by CCK-8. Results The particle size of 5-FU encapsulated ethosomes was (87.5 ±5.4) nm, the polydispersion index was 0.151 ±0.27, and the encapsulation efficiency of 5-FU was (10.03 ±2.12)%. CLSM micrographs showed that ethosomes facilitated the penetration of RhoB into the cell, as evident from the high intensity fluorescence. In comparison, when incorporated in hydroalcoholic solution, very weak fluorescence was detected. The optical density (OD) of unit area was calculated through the Image-Pro Plus 6.0 image analysis software, and the OD value of ethosomal group was distinguished higher than hydroalcoholic group (P<0.01). With the increase of the concentration of 5-FU, growth inhibition rate of fibroblast affected by 5-FU HA and 5-FU ES was gradually enhanced. The IC50 of 5-FU encapsulated ethosomes and 5-FU hydroalcoholic solution to fibroblasts were (9.2582±1.2329) μg/mL and (18.0352±2.3145) μg/mL, respectively, the difference between the two groups was distinguished (P<0.05). Meanwhile, ethosomal carrier was not toxic to the cultured cells. Conclusion Ethosomes can effectively enhance intracellular delivery of 5-FU, as a result can enhance the inhibitory effect of 5-FU on fibroblast viability. Meanwhile, ethosomal carrier was not toxic to the cultured cells. Ethosomes as percutaneous drug delivery carriers, are also a promising candidate for the delivery of biological and chemical compounds to cultured cells. Related Articles | Metrics

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Clonal Analysis on Low Density Culture of Keloid Fibroblasts QU Miao,WU Xiaoli,CHAI Gang 2014, 10 (2):  82-99.  DOI: 10.3969/j.issn.1673-0364.2014.02.005 Abstract ( 370 )   Objective To compare the clone forming capacity between keloid fibroblasts (KFs) and normal dermal fibroblasts (NFs), and to explore whether there are pathological stem cells in keloid and the role of keloid stem cells on the mechanism of keloid formation. Methods Primary cells were obtained from keloid tissue and normal dermis respectively through enzyme digestion method, and then were cultured under low-density conditions of 4 000/dish. Two weeks later, the clone formation and morphology were determined. Results Under low-density culture conditions, clones were formed in both KFs and NFs. KFs formed distinct and dense clones while NFs formed loose and fewer ones. KFs exhibited higher clone forming efficiency (CFE) of (0.80±0.21)%than NFs with CFE of (0.18±0.06)% (P<0.05). Conclusion With low-density culture, keloid fibroblasts possess higher level of cloning capacity than normal dermal fibroblasts, which suggests pathological stem cells may exist in keloid tissues. Related Articles | Metrics

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Biomechanical Study of Nasal Deformation in Patients with Unilateral Cleft Lip Based on Finite Element Models DONG Lei,WANG Shengzhang,SONG Jianxing 2014, 10 (2):  85-88.  DOI: 10.3969/j.issn.1673-0364.2014.02.006 Abstract ( 365 )   Objective To explore the cause and to reveal the biomechanical regularity of nasal deformation in patients with unilateral cleft lip through the biomechanical analysis on nasal deformation by finite element method, in order to establish a good biomechanical theoretical basis for treating nasal deformation of unilateral cleft lip. Methods From 2007 to 2009, 10 cases of unilateral cleft lip with nasal deformation were collected. All the cases were scanned by CT and MRI preoperatively, image data were collected and the three-dimensional nasal deformation were reconstructed by the software MIMICS. Then the finite element models of nasal deformation in unilateral cleft lip were established and the model's constraint conditions were defined for the biomechanical measurement and analysis of nasal deformation in unilateral cleft lip. Results The three-dimensional model of nasal deformation in unilateral cleft lip were reconstructed successfully. The biomechanical analysis showed that, under the static state, nasal deformation had a very small value of stress distribution. The value of the key stress points were as follows:nasal septum 0.009 35 ±0.002 MPa, the bottom of nasal columella 0.005 9 ±0.002 1 MPa, lateral feet of nasal alar 0.006 81±0.001 3 MPa;Under displacement load status, the deformed nasal had a large value of the stress distribution. The value of the key stress points were as follows:ipsilateral nasal columella 25.51 ±3.98 MPa, nasal septum 7.882 ±1.35 MPa, ipsilateral nasal alar 8.184 ±1.58 MPa. Conclusion Affected side of nasal columella is the key mechanical part to rectify deformed nose, and the following part is ipsilateral nasal alar; The stress concentration of nasal septum suggests that the rectification of nasal septum is very important, its rectification and fixation are perhaps the important contents of deformed nose rectification. Related Articles | Metrics

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Clinical Application of Body-jet Hydrodynamic Liposuction System in Breast Augmentation with Autologous Fat Transplantation ZUO Zongbao,SUN Jiaming,GUO Ke,GUO Nengqiang 2014, 10 (2):  89-91.  DOI: 10.3969/j.issn.1673-0364.2014.02.007 Abstract ( 305 )   Objective To explore the clinical application and postoperative effects of Body-jet hydrodynamic liposuction system in breast augmentation with autologous fat transplantation. Methods From September 2012 to December 2013, 18 cases received breast augmentation with autologous fat transplantation using Body-jet hydrodynamic liposuction system. The autologous fat was collected and purified by Body-jet hydrodynamic liposuction system, then was injected to each layer of breast tissue multi-levelly and multi-dimensionally. The fat dosage of unilateral breast injection ranged from 100-250 mL. Results After 6 to 12 months' follow-up, all 18 cases achieved good results including full-round contour, natural breast shape and soft feeling of palpation. Satisfactory outcome were received without severe complications. Conclusion Body-jet hydrodynamic liposuction technique is safe, effective, easy to operate, and is an ideal method for breast augmentation with autologous fat transplantation. Related Articles | Metrics

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Heating and Bandage Treatment on Patients with Chronic Lymphedema of Extremities LI Ke,LIU Ningfei,FU Lanfen,WANG Li,CHEN Jiajia,LIANG Chen,ZHANG Yixin 2014, 10 (2):  92-95.  DOI: 10.3969/j.issn.1673-0364.2014.02.008 Abstract ( 479 )   Objective To investigate the effects of heating and bandage treatment on patients with chronic lymphedema of extremities. Methods From January 2012 to September 2013, 53 patients with chronic lymphedema of extremities were received heating and bandage treatment. The extent of tissue edema were detected by Multiple-frequency bioelectrical impedance analysis before and after treatment. The circumference of the extremities were measured before and after treatment. Results After heating and bandage treatment, remarkable reduction of accumulated edema fluid in tissue and circumference were observed (P=0.006, P=0.000). On long-term follow-up, most of the patients felt better. The relapse of lymphedema was reduced by pressure therapy with elastic material secondly. The correlation between edema lessening and circumference reducing was obviously significant (r=0.356, P=0.01). Conclusion Heating and bandage treatment can effectively reduce the edema of extremities. Simultaneously, the subsequent elastic material pressure therapy was essential. Related Articles | Metrics

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The Change of the Female Facial Soft Tissue after Mandibular Angle Osteotomy XU Yourong,CHAI Gang,ZHANG Yan,HOU Yikang,QU Miao,SHEN Congcong 2014, 10 (2):  96-99.  DOI: 10.3969/j.issn.1673-0364.2014.02.009 Abstract ( 381 )   Objective To observe the change of facial soft tissue after mandibular angle osteotomy. Methods Comuterized tomography (CT) examination was performed on 130 patients treated by mandibular angle ostemomy before operation and 6 months after operation, respectively. Intra-oral incision were used for correcting prominent mandibular angle (PMA).①ratio of the height from lobule to mandibular angle&the height of lower face, ②ratio of the distance between the two sides of mandibular angle&the distance between the two sides of zygometic arch and ③the degree of mandibular angle were all calculated pre-and postoperatively to evaluate the change of facial soft tissue. Results The preoperative ratio of the height from lobule to mandibular angle&the height of lower face was 1∶(1.5±0.12), while the postoperative was 1∶(1.92±0.15);The preoperative ratio of the distance between the two sides of mandibular angle & the distance between the two sides of zygometic arch was 1∶(1.03±0.11), while the postoperative was 1∶(1.2±0.21);The preoperative mandibular angle was (109±1.7) °, while the postoperative was (121 ±2.1) ° . Conclusion Six months after mandibular angle osteotomy, the patients with mandibular angle hypertrophy could obtain better quantitative improvement of facial shape. Related Articles | Metrics

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Comparative Effectiveness of INTERTAN Intramedullary Nail and Sliding Hip Screw in Treating Intertrochanteric or Subtrochanteric Fracture of Elderly Patients ZHANG Wei,ZOU Jian 2014, 10 (2):  100-103.  DOI: 10.3969/j.issn.1673-0364.2014.02.010 Abstract ( 343 )   Objective To compare the surgical efficacy between INTERTAN intramedullary nail and sliding hip screw in treating intertrochanteric or subtrochanteric fracture of old people. Methods From Jan 2010 to Jan 2012, 120 patients with Intertrochanteric or subtrochanteric Fractures were treated by INTERTAN intramedullary nail ( n=67) and sliding hip screw (n=53). Operation time, bleeding volume, amount of drainage, visual analogue scale (VAS) pain score, Harris hip score, SF-36 score and complications were recorded at each time points. Results Among all the patients, 111 patients (INTERTAN group: 64 cases; DHS group: 47 cases) were followed up for more than 1 year successfully. Bleeding volume and amount of drainage in INTERTAN group were larger than in DHS group. VAS score in INTERTAN group 3 days after operation was better than in DHS group, and there was no difference between the two groups 3 and 12 months after operation. There was no difference between the two groups in operation time, Harris hip score, SF-36 score and complications during the time of follow-up. Conclusion INTERTAN nails and sliding hip screws are similar in terms of pain, function, and comlications rates in treating intertrochanteric or subtrochanteric fractures. Related Articles | Metrics

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Postauricular Subcutaneous Pedicle Flap in One Stage Reconstruction of Retroauricula Defect ZUO Zongbao,SUN Jiaming,GUO Ke,GUO Nengqiang,NING Jinlong 2014, 10 (2):  104-120.  DOI: 10.3969/j.issn.1673-0364.2014.02.011 Abstract ( 341 )   Objective To investigate the surgical efficacy of the postauricular subcutaneous pedicle flap in one stage reconstruction of retroauricula defect. Methods From September 2011 to September 2013, 16 cases with retroauricula defects were repaired by the postauricular subcutaneous pedicle flap in one stage reconstruction. The maximum size of defect was 2.2 cm×2.5 cm, and the minimum was 1.0 cm×1.5 cm. The surgical efficacy was observed after operation. Results All the postauricular flaps were survived postoperatively, and the auricular appearances of all the patients were satisfactory without visible scar during 2 to 12 months ’ follow up. Conclusion Postauricular subcutaneous pedicle flap, with reliable blood supply, similar contour, color, texture with surrounding tissue, and hidden donor site, is one of the best methods for the reconstruction of preauricular defects. Related Articles | Metrics

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The Conditions of Induced Differentiation of Adipose Derived Stem Cells in Vitro GAO Yu,YU Qingxiong,LI Qingfeng 2014, 10 (2):  106-109.  DOI: 10.3969/j.issn.1673-0364.2014.02.012 Abstract ( 496 )   Adipose derived stem cells (ADSC), a type of pluripotent stem cells, are isolated from adipose tissue and can be induced into adipose cells, osteoblasts, cartilage cells, muscle cells, nerve cells and so on. Many factors are involved in this process. In this paper, the biological characteristics, the conditions and factors that related to the differentiation of ADSC were reviewed. Related Articles | Metrics

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Changes of Histomorphology and Cytokines after Tissue Expansion SONG Xiaodong,LV Changsheng 2014, 10 (2):  110-112.  DOI: 10.3969/j.issn.1673-0364.2014.02.013 Abstract ( 275 )   Soft tissue expansion is mainly about a series changes of histomorphology and cytokines under mechanical stress, and it provides research foundation of rapid tissue expansion. In this paper, the changes of histomorphology and cytokines after tissue expansion were reviewed. Related Articles | Metrics

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Progress in Treatment of Neurofibromatosis Type 1 GAO Yang,HU Xiaojie,LIN Xiaoxi 2014, 10 (2):  113-116.  DOI: 10.3969/j.issn.1673-0364.2014.02.014 Abstract ( 421 )   Neurofibromatosis type 1 is an autosomal dominant genetic disease, and can cause multisystem disorders affecting skin, bone, vision and internal organs. It is difficult to cure. In this paper, the indications, therapy attentions and development direction of different treatment methods were summarized. Related Articles | Metrics

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Clinical Research Progress of Muller-Weiss Disease YU Weilin,SONG Guoxun,SHI Zhongmin 2014, 10 (2):  117-120.  DOI: 10.3969/j.issn.1673-0364.2014.02.015 Abstract ( 501 )   Muller-Weiss disease mostly strike the feet of middle and aged women. The cause of the disease is always controversial, it is now generally believed that navicular bone dysplasia and the abnormal stress distribution on the surface of navicular bone are the main reasons. The major clinical manifestations of the disease are the dorsal foot pain of load-bearing and progressive “flatfoot-varus” deformity. Surgical treatment is an effective way to treat Muller-Weiss disease. In this paper, clinical research progress of Muller-Weiss disease was reviewed. Related Articles | Metrics