Journal of Diagnostics Concepts & Practice ›› 2021, Vol. 20 ›› Issue (06): 557-561.doi: 10.16150/j.1671-2870.2021.06.008
• Original articles • Previous Articles Next Articles
Received:
2021-04-01
Online:
2021-12-25
Published:
2021-12-25
Contact:
ZHU Yan
E-mail:zhuyan416@126.com
CLC Number:
WANG Mingyi, ZHU Yan. Construction of fluorescent human zona pellucida fusion protein expression vectors and expression in CHO cells[J]. Journal of Diagnostics Concepts & Practice, 2021, 20(06): 557-561.
名称 | 引物序列(5’→3’) | 长度 (bp) | 退火 温度 |
---|---|---|---|
ZP1 | |||
片段1 | F-TAAGTGGGATCCATGGCAGGA R-TTGGACTGGGCGCTGTGCC | 126 | 59 ℃ |
片段2 | F-GGCACAGCGCCCAGTCCAA R-CCCTTGATCCCACAGTCGTA | 695 | 60 ℃ |
片段3 | F-TACGACTGTGGGATCAAGGG R-CACTTACTCGAGTCACTGTC | 1 975 | 58 ℃ |
ZP2 | |||
片段1 | F-TAAGTGGGATCCATGGCGTG R-CTTGGACTGGGCTGGAAAGGCA | 156 | 58 ℃ |
片段2 | F-TGCCTTTCCAGCCCAGTCCAAG R-CATCGCAAGTGACAGTGCC | 697 | 59 ℃ |
片段3 | F-GGCACTGTCACTTGCGATG R-CAGCTACTCGAGTTAGTGAT | 2 098 | 58 ℃ |
ZP3 | |||
片段1 | F-TAAGTGGGATCCATGGAGCTGAG R-TCGCCCTTGCTCTGTACGGACG | 128 | 59 ℃ |
片段2 | F-CGTCCGTACAGAGCAAGGGCGA R-TGACACTCCACCAGTACGGG | 715 | 60 ℃ |
片段3 | F-CCCGTACTGGTGGAGTGTCA R-GAAGTGCTCGAGTTATTCGG | 1 163 | 59 ℃ |
ZP4 | |||
片段1 | F-TAAGTGGGATCCATGTGGCTGCT R-CCTCGCCCTTGCTCACACTGGAA | 102 | 58 ℃ |
片段2 | F-TTCCAGTGTGAGCAAGGGCGAGG R-ACAGTGGAGCACCTTGTACA | 719 | 60 ℃ |
片段3 | F-TGTACAAGGTGCTCCACTGT R-CGGCTGTCTAGATTATTGAC | 1 542 | 59 ℃ |
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