Exosomal miRNA146b-5p promotes migration and invasion of papillary thyroid carcinoma cell

doi:10.16139/j.1007-9610.2019.04.014

Abstract

Abstract: Objective To isolate and identify the exosomes secreted by papillary thyroid carcinoma (PTC) KTC-1 cells, and to investigate the effects of miRNA146b-5p in exosomes on the migration and invasion in vitro. Methods Exosomes secreted by KTC-1 cells were collected and identified. miRNA 146b-5p and its inhibitor were electroporated into exosomes and co-cultured with KTC-1 cells. Migration and invasion in vitro were examined. Results Exosomes secreted by KTC-1 cells were successfully isolated and identified. Exosomal miRNA146b-5p could enter KTC-1 cells and promote migration and invasion of KTC-1 cells in vitro. Conclusions Exosomal miRNA146b-5p can promote the migration and invasion of PTC in vitro. miRNA146b-5p silencing in exosomes might represent a potential approach against PTC.

Key words: Papillary thyroid carcinoma, Exosome, miRNA146b-5p, Migration and invasion

CLC Number:

R736.1

YE Weidong, DENG Xianzhao, FAN Youben. Exosomal miRNA146b-5p promotes migration and invasion of papillary thyroid carcinoma cell[J]. Journal of Surgery Concepts & Practice, 2019, 24(04): 344-348.

References

[1] Kitahara CM, Sosa JA.The changing incidence of thyroid cancer[J]. Nat Rev Endocrinol,2016,12(11):646-653.
[2] Siegel RL, Miller KD, Jemal A. Cancer Statistics,2017[J]. CA Cancer J Clin,2017,67(1):7-30.
[3] Pellegriti G, Frasca F, Regalbuto C, et al.Worldwide increasing incidence of thyroid cancer: update on epidemio-logy and risk factors[J]. J Cancer Epidemiol,2013,2013:965212.
[4] Haugen BR, Alexander EK, Bible KC, et al.2015 Ame-rican Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American Thyroid Association guidelines task force on thyroid nodules and differentiated thyroid cancer[J]. Thyroid,2016,26(1):1-133.
[5] Guilmette J, Nosé V.Hereditary and familial thyroid tumours[J]. Histopathology,2018,72(1):70-81.
[6] Xing M.Molecular pathogenesis and mechanisms of thyroid cancer[J]. Nat Rev Cancer,2013,13(3):184-199.
[7] Riesco-Eizaguirre G, Santisteban P.ENDOCRINE TUMOURS: Advances in the molecular pathogenesis of thyroid cancer: lessons from the cancer genome[J]. Eur J Endocrinol,2016,175(5):R203-R217.
[8] Johnstone RM.Exosomes biological significance: A concise review[J]. Blood Cells Mol Dis,2006,36(2):315-321.
[9] Milane L, Singh A, Mattheolabakis G, et al.Exosome mediated communication within the tumor microenvironment[J]. J Control Release,2015,219:278-294.
[10] Seo N, Akiyoshi K, Shiku H.Exosome-mediated regulation of tumor immunology[J]. Cancer Sci,2018,109(10):2998-3004.
[11] Cui S, Cheng Z, Qin W, et al.Exosomes as a liquid biopsy for lung cancer[J]. Lung Cancer,2018,116:46-54.
[12] Sun D, Zhuang X, Zhang S, et al.Exosomes are endogenous nanoparticles that can deliver biological information between cells[J]. Adv Drug Deliv Rev,2013,65(3):342-347.
[13] Kamerkar S, Lebleu VS, Sugimoto H, et al.Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer[J]. Nature,2017,546(7659):498-503.
[14] El-Andaloussi S, Lee Y, Lakhal-Littleton S, et al.Exosome-mediated delivery of siRNA in vitro and in vivo[J]. Nat Protoc,2012,7(12):2112-2126.
[15] Marqués-García F, Isidoro-García M.Protocols for Exosome Isolation and RNA Profiling[J]. Methods Mol Biol,2016,1434:153-167.
[16] Théry C, Amigorena S, Raposo G, et al. Isolation and characterization of exosomes from cell culture supernatants and biological fluids[J]. Curr Protoc Cell Biol,2006,Chapter 3:Unit 3.22.
[17] Di Leva G, Garofalo M, Croce CM.MicroRNAs in cancer[J]. Annu Rev Pathol,2014,9:287-314.
[18] Zhu Y, Wu G, Yan W, et al.miR-146b-5p regulates cell growth, invasion, and metabolism by targeting PDHB in colorectal cancer[J]. Am J Cancer Res,2017,7(5):1136-1150.
[19] Lin F, Wang X, Jie Z, et al.Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16[J]. J Huazhong Univ Sci Technolog Med Sci,2011,31(4):509.
[20] Li Y, Zhang H, Dong Y, et al.MiR-146b-5p functions as a suppressor miRNA and prognosis predictor in non-small cell lung cancer[J]. J Cancer,2017,8(9):1704-1716.
[21] Lima CR, Geraldo MV, Fuziwara CS, et al.MiRNA-146b-5p upregulates migration and invasion of different papillary thyroid carcinoma cells[J]. BMC Cancer,2016,16:108.
[22] Geraldo MV, Fuziwara CS, Friguglieti CU, et al.MicroRNAs miR-146-5p and let-7f as prognostic tools for aggressive papillary thyroid carcinoma: a case report[J]. Arq Bras Endocrinol Metabol,2012,56(8):552-557.
[23] Deng X, Wu B, Xiao K, et al.MiR-146b-5p promotes metastasis and induces epithelial-mesenchymal transition in thyroid cancer by targeting ZNRF3[J]. Cell Physiol Biochem,2015,35(1):71-82.
[24] Lee JC, Zhao JT, Gundara J, et al.Papillary thyroid cancer-derived exosomes contain miRNA-146b and miRNA-222[J]. J Surg Res,2015,196(1):39-48.
[25] Samsonov R, Burdakov V, Shtam T, et al.Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer[J]. Tumour Biol,2016,37(9):12011-12021.