|
The Effect of PPAR-Gamma Signaling Pathway in the Adipogenic Differentiation of Hemangioma Derived Mesenchymal Stem Cells in Vitro
XU Yuan,GUO Yao,YUAN Siming,WANG Min,CHEN Haini,SHEN Weimin
2019, 15 (1):
1-9.
DOI: 10.3969/j.issn.1673-0364.2019.01.001
Objective To investigate the effect of PPAR-gamma signaling pathway in the adipogenic differentiation of hemangioma derived mesenchymal stem cells(Hem-MSCs)in vitro.Methods Hem-MSCs were cultured in 5 groups of different media,including group A(ordinary culture medium),group B(ordinary culture medium+rosiglitazone),group C(adipogenic medium),group D(adipogenic medium+rosiglitazone),group E(adipogenic medium+rosiglitazone+GW9662).The adipogenic differentiation of hem-MSCs was observed by light microscope,and the range and number of lipid droplets were observed by oil red O staining.The expression of perilipin A in each group was detected by Western-blot after two weeks of culture to quantify the degree of adipogenic differentiation.The expressions of adipogenesis-related genes(CEBPA,LPL,PLIN1,PPARG)of each group at different time points were detected by real-time quantitative PCR.Results Oil red O staining showed that rosiglitazone could promote the adipogenic differentiation of hem-MSCs in adipogenic medium.Western-blot confirmed that rosiglitazone could significantly promote the expression of perilipin A protein in the induced differentiation group.Real-time quantitative PCR further confirmed that rosiglitazone could promote the expression of adipogenesis-related genes(CEBPA,LPL,PLIN1,PPARG)in hem-MSCs at the first and second weeks.GW9662,an antagonist of PPAR-gamma signaling pathway,could block the above effects.Conclusion Activated PPAR-gamma signaling pathway promotes the adipogenic differentiation of Hem-MSCs in vitro.
Related Articles |
Metrics
|