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20 February 2019, Volume 15 Issue 1    Next Issue

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The Effect of PPAR-Gamma Signaling Pathway in the Adipogenic Differentiation of Hemangioma Derived Mesenchymal Stem Cells in Vitro XU Yuan,GUO Yao,YUAN Siming,WANG Min,CHEN Haini,SHEN Weimin 2019, 15 (1):  1-9.  DOI: 10.3969/j.issn.1673-0364.2019.01.001 Abstract ( 509 )   Objective To investigate the effect of PPAR-gamma signaling pathway in the adipogenic differentiation of hemangioma derived mesenchymal stem cells(Hem-MSCs)in vitro.Methods Hem-MSCs were cultured in 5 groups of different media,including group A(ordinary culture medium),group B(ordinary culture medium+rosiglitazone),group C(adipogenic medium),group D(adipogenic medium+rosiglitazone),group E(adipogenic medium+rosiglitazone+GW9662).The adipogenic differentiation of hem-MSCs was observed by light microscope,and the range and number of lipid droplets were observed by oil red O staining.The expression of perilipin A in each group was detected by Western-blot after two weeks of culture to quantify the degree of adipogenic differentiation.The expressions of adipogenesis-related genes(CEBPA,LPL,PLIN1,PPARG)of each group at different time points were detected by real-time quantitative PCR.Results Oil red O staining showed that rosiglitazone could promote the adipogenic differentiation of hem-MSCs in adipogenic medium.Western-blot confirmed that rosiglitazone could significantly promote the expression of perilipin A protein in the induced differentiation group.Real-time quantitative PCR further confirmed that rosiglitazone could promote the expression of adipogenesis-related genes(CEBPA,LPL,PLIN1,PPARG)in hem-MSCs at the first and second weeks.GW9662,an antagonist of PPAR-gamma signaling pathway,could block the above effects.Conclusion Activated PPAR-gamma signaling pathway promotes the adipogenic differentiation of Hem-MSCs in vitro. Related Articles | Metrics

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Effectiveness of BK Channel Inhibitor on Proliferation,Apoptosis and Cytokines of Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton's Jelly SONG Ahui,TONG Yan,LIU Yingli 2019, 15 (1):  5-9.  DOI: 10.3969/j.issn.1673-0364.2019.01.002 Abstract ( 409 )   Objective To explore the effects of BK channels inhibitors on cell activity,apoptosis and cytokine secretion of mesenchymal stem cells derived from human umbilical cord Wharton's jelly(WJ-MSC).Methods The WJ-MSC were isolated and cultured by tissue adherent method.The expression of BK channels was detected by Western-blot.After treated with different concentrations of BK channels inhibitors,the activity of cells was detected by CCK-8,the apoptosis of cells was detected by Annexin-V-FITC/PI,and the expression of cytokine(IL6,IL10)was detected by Elisa.Results BK channels expression was found in WJ-MSC.BK channels inhibitors reduced cell activity,promoted cell apoptosis and secretion of cytokine(IL6),all of which have statistical significance(P<0.05).But BK channels inhibitors did not increase the secretion of IL-10.Conclusion BK channels inhibitors can inhibit the activity of WJ-MSC,promote cell apoptosis and promote the secretion of IL6. Related Articles | Metrics

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Applied Anatomy of the Facial Artery Branches in Mandibular Osteotomy WU Yingzhi,PAN Sida,MU Xiongzheng 2019, 15 (1):  10-12.  DOI: 10.3969/j.issn.1673-0364.2019.01.003 Abstract ( 535 )   Objective To determine the safe range of mandibular osteotomy and improve the surgical safety by exploring the anatomy of the facial artery branches.Methods Eight corpses were dissected for both sides.The position where the facial artery turned out along the border of mandible,the distance between the submandibular gland artery and submental artery,the distance between the start of submental artery and the border of mandible were all measured to investigate the routines of Chinese facial artery and its branches submandibular gland artery and submental artery,and to compare the variation of bilateral routines.Results Facial artery turned out from lateral 1/3 between mandibular angle point and the intersection of the vertical line from mouth corner to the mandible border.The submental artery was turned out with an average distance of 1.05 cm below the border of mandible.The submandibular gland artery started with an average distance of 0.95 cm below the submental artery.Conclusion The reciprocate saw is relatively safe to use 1.05 cm away from the border of mandible. Related Articles | Metrics

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Preliminary Study of SVF-gel Promoting Wound Healing in Diabetic Rats KUAI Quan,WANG Yimei,LI Cong,HUANG Na,ZHU Yuan,WEN Huicai 2019, 15 (1):  13-16.  DOI: 10.3969/j.issn.1673-0364.2019.01.004 Abstract ( 373 )   Objective To investigate the effect and mechanism of SVF-gel promoting wounds healing in diabetic rats.Methods Thirty-six male SD rats were prepared by intraperitoneal injection of Streptozotoein(55 mg/Kg)to establish the diabetic model.The wound with a diameter of 0.6 cm was made on both sides of diabetic rats after one week.Then those rats were divided into 3 groups(n=12)randomly,and treated with SVF-gel(Group A),SVF(Group B),and normal saline(Group C)respectively.The wound sites were observed and the percentage of wound closure was calculated.HE staining and immunohistochemical staining were used to observe the morphological changes of granulation tissue in the wound and the angiogenesis.Results The wound healing rates at all time points of group A and group B were higher than that of group C(P<0.05),and the wound healing rates at all time points of group A were higher than that of group B(P<0.05).Histological changes:Compared with group C,the infiltration of inflammatory cells was decreased in group A and group B,the degree of reepithelialization was obvious,the angiogenesis was significantly increased,the granulation tissue structure was dense,and these histological changes were the most obvious in group A.Conclusion For the wounds of diabetic mice,SVF-gel may increase the formation of blood vessels and the granulation tissue,reduce the inflammatory reaction to promote the wound healing. Related Articles | Metrics

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Clinical Application of Gemstone Spectral CT Imaging for Reducing Orbital Metal Artifacts WU Jian,XU Yemin,HAN Yongshun,TAO Xiaofeng 2019, 15 (1):  17-19.  DOI: 10.3969/j.issn.1673-0364.2019.01.005 Abstract ( 394 )   Objective To evaluate the practical value of gemstone spectral imaging(GSI)in reducing the metal artifacts of orbital implants.Methods GSI examination were performed in 32 patients with metal implants of orbit,and the polychromatic images(140 kVp)were obtained.Gemstone spectral imaging(GSI)viewer was used to obtain 11 sets of monochromatic images(40-140 keV with interval of 10 keV).Subjective image quality of two methods was assessed by 3 radiologists in consensus.CT value and noise value(SD)of ROI were measured at the most obvious and non-artifact level of each image,and contrast noise ratio(CNR)and artifact index(AI)were calculated.The SD,CNR and AI values were compared between polychromatic images and monochromatic images.Results Among the 11 sets of monochromatic images,the noise value(SD)and artifact index(AI)of 110 keV images were the smallest,and the contrast noise ratio(CNR)was the best.Compared with the polychromatic images,the artifacts of orbital metal implants were removed significantly,and the image quality was improved significantly.Conclusion Gemstone spectral CT can effectively reduce artifacts of orbital metal implants and will provide high image quality in the area of metal implants.110 keV is the best monochromatic imaging point for the image quality of patients with orbital metal implant. Related Articles | Metrics

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Bipedicled Pre-Expended Superficial Temporary Artery Visor Flap for Post-Burn Reshaping of Lower Facial Contour MIN Peiru,ZHANG Yixin 2019, 15 (1):  20-24.  DOI: 10.3969/j.issn.1673-0364.2019.01.006 Abstract ( 338 )   Objective To explore the clinical application and outcome of the bipedicled pre-expended superficial temporary artery visor flap in the post-burn reshaping of lower facial contour.Methods From January 2014 to January 2017,10 male patients were treated with bipedicled pre-expended superficial temporary artery visor flap for the coverage of the defect in upper and lower lips.After inserting the tissue expander below scalp in first stage,a period of 6-8 months was conducted for tissue expansion.Second stage operation was then applied based on the principle of subunits.Results All the flap survived and covered the upper and lower lip area without any postoperative complications.All patients were followed up for 6-12 months.The flap was soft and not puffy.The skin color was similar to that around the lip.The function of mouth opening and expression was good without obvious scars.Conclusion The bipedicled pre-expended superficial temporary artery visor flap is safe and effective in the reconstruction of skin defect of upper and lower lips for male patients. Related Articles | Metrics

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Vacuum Sealing Drainage Combined with Autologous Platelet-rich Gel for the Treatment of Diabetic Foot XIE Juan,FANG Ying,CAO Dongsheng,LU Yang 2019, 15 (1):  25-28.  DOI: 10.3969/j.issn.1673-0364.2019.01.007 Abstract ( 401 )   Objective To observe the clinical effects of vacuum sealing drainage(VSD)combined with autologous platelet-rich gel(APG)for the treatment of diabetic foot.Methods From July 2016 to February 2018,60 patients with diabetic foot ulcer(DFU)were randomly divided into two groups:experimental group(n=30)and control group(n=30).The experimental group was treated by VSD combined with APG,while the control group was treated by VSD only.The cure rate,healing time,time of bacterial turnover and histological appearance of the two groups were compared.Results The cure rate of the experimental group was higher than that of the control group at all time points(P<0.05).The time of wound healing and bacterial turnover in experimental group were both lower than that in control group(P<0.05).Conclusion VSD combined with APG for the treatment of DFU is effective in promoting the wound healing and shortening the healing time. Related Articles | Metrics

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Application of Medial Plantar Flap in the Repair of Peri-Ankle Soft Tissue Defects CANG Zhengqiang,NI Xiaodong,WANG Min,XU Yuan,CUI Lei,YUAN Siming 2019, 15 (1):  29-31.  DOI: 10.3969/j.issn.1673-0364.2019.01.008 Abstract ( 347 )   Objective To investigate the application of medial plantar flap in the repair of peri-ankle soft tissue defects.Methods From September 2014 to September 2018,a total of 9 cases with peri-ankle soft tissue defects were included in this retrospective study.The defects were located in the inner ankle(n=6)and anterior ankle(n=3).The size of the defects varied from 5 cm×4 cm to 10 cm×8 cm.Results All flaps and skin grafts survived well.Patients were followed up for 6-24 months,all flaps had good color,texture and protective sensation.All patients were able to walk pain-free in normal shoes.Conclusion Medial plantar flap is a good choice for repairing the small to medium size defects around ankle. Related Articles | Metrics

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Clinical Study of Rhinoplasty with Autologous Rib Cartilage and Facial Fat Grafting to Rebuild Facial Balance LI Jie,YANG Xiaoning,LI Xin,WANG Chunhu,WANG Keming,MA Jiguang,LI Zhanqiang 2019, 15 (1):  32-35.  DOI: 10.3969/j.issn.1673-0364.2019.01.009 Abstract ( 396 )   Objective Based on the guidelines of aesthetics,to rebuild facial balance through rhinoplasty with autologous rib cartilage and facial fat grafting.Methods For patients with low nose and short nose,rhinoplasty with autologous rib cartilage and facial fat grafting were performed at the same time to correct the imbalance of facial contour caused by the nasal profile being too obtrusive after simple rhinoplasty.The results were photographed and recorded,and the postoperative satisfaction rate and complications were followed up.Results In this study,288 patients were included.The follow-up period ranged from 6 months to 5 years.The postoperative nasal appearance was improved and the overall facial contour was balanced.No serious complications were observed.The results were satisfactory and the satisfaction rate was 86%.Conclusion Rhinoplasty with autologous rib cartilage and facial fat grafting can not only improve the appearance of the nose,but also reshape the balance of the face,and make the face harmonious and unified again. Related Articles | Metrics

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Application of Autologous Fat Granule Transplantation in Facial Contouring XIONG Junwen,CHEN Shouzheng,XU Wenjie 2019, 15 (1):  36-38.  DOI: 10.3969/j.issn.1673-0364.2019.01.010 Abstract ( 346 )   Objective To evaluate the clinical efficacy and safety of autologous fat granule transplantation in facial contouring.Methods From May 2013 to May 2016,70 patients treated with autologous fat granule transplantation were selected.Subcutaneous fat was injected into facial regions after centrifugation and purification process through subcutaneous tumescent liposuction.The clinical data,complications and the satisfaction of the patients were analyzed retrospectively.Results All operations were successfully completed;After 6 months of postoperative follow-up,no infection,fat liquefaction,necrosis,induration?and embolism were observed;The satisfaction rate of the patients were 94.85%and 97.94%at 1 month and 6 month after operation respectively.Conclusion Autologous fat granule transplantation is effective in facial contouring with high success rate and low complication rate.It is worthy of clinical promotion. Related Articles | Metrics

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Evaluation of Retention Efficiency of Different Connection Patterns in Restoring Subtotal Bilateral Maxilla Defection by Zygomatic Implants Supported Prosthetics ZHAO Xiaomei,ZHANG Chenping,LIU Jiannan,WANG Zhen 2019, 15 (1):  39-45.  DOI: 10.3969/j.issn.1673-0364.2019.01.011 Abstract ( 455 )   Objective To explore the ideal connection pattern of zygomatic implants supported prosthetics for functional restoration of maxillary defection by comparing the retentive efficiency of different connection pattern.Methods The patents'functional rehabilitations such as appearance,chewing function,pronunciation in 10 maxilla defection patients restored with two different connection types of zygomatic implants supported prosthetics were evaluated quantitatively,and the repair effects of the two connection patterns were compared.Results Comparing with magnetic attachment,galvanized golden frames and milling bar had higher score of dislocation during chewing movement.Other scores were not significantly different.Conclusion Galvanized golden frames and milling bar is the ideal connection pattern of zygomatic implants supported prosthetics for functional restoration of maxillary defection. Related Articles | Metrics

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Small Needle Knife-Assisted Aluronic Acid Combined with Botulinum Toxin Injection in the Treatment of Glabellar Frown Line LUO Huiying,TANG Songjia,ZHANG Jufang 2019, 15 (1):  43-45.  DOI: 10.3969/j.issn.1673-0364.2019.01.012 Abstract ( 501 )   Objective To explore the effect of small needle knife-assisted aluronic acid combined with botulinum toxin injection in the treatment of glabellar frown line.Methods Fifteen patients with severe glabellar frown line were included and treated with aluronic acid injection assisted by small needle knife for subcutaneous release,and botulinum toxin was injected later.Global Aesthetic Improvement Scale(GAIS)was used to assess the effect.Results Aesthetic improvements were observed.The effective rate was 86.7%.Conclusion Small needle knife-assisted aluronic acid combined with botulinum toxin injection is convenient,safe and effective in the treatment of glabellar frown line.It is worthy of clinical promotion. Related Articles | Metrics

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Application of Collaboration Care Model in Postoperative Nursing of Children with Hemifacial JIANG Lihua,ZHOU Yanchun,HUANG Ying 2019, 15 (1):  46-51.  DOI: 10.3969/j.issn.1673-0364.2019.01.013 Abstract ( 387 )   Objective To explore the effect of collaboration care model(CCM)in postoperative care for children with hemifacial microsomia(HM).Methods From June 2016 to June 2017,totally 100 children with HM who received extender implantation were randomly selected and divided equally into experimental group and control group by convenience sampling.The children in control group were treated with?routine nursing,while children in observation group were treated with CCM nursing intervention on the basis of routine nursing.The scores of nursing effect,oral cleanliness and compliance were compared between the two groups.Results The scores of nursing?effect,oral cleanliness and compliance in the experimental group were all significantly higher than those in the control group(P<0.05).Conclusion CCM can significantly improve the postoperative nursing effect of children with hemifacial microsomia,which is worthy of clinical application. Related Articles | Metrics

Review

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The Effect of Subpopulations of Dermal Skin Fibroblastsin in Skin Regeneration and Maintaining Homeostasis TAN Pohching,ZHOU Shuangbai,LI Qingfeng 2019, 15 (1):  49-51.  DOI: 10.3969/j.issn.1673-0364.2019.01.014 Abstract ( 542 )   Dermal fibroblasts divide into distinct subpopulation lineages:papillary fibroblasts(Fp)in superficial dermis layer,and reticular fibroblasts(Fr)in deep dermis layer.The two lineages have differences not only in cell functions and differentiation potentials,but also in wound healing,hair follicle formation,neogenesis,and skin senescence.In this paper,the differences of cell morphology,function,source,differentiation,specific expression between the two lineages,and their role in skin regeneration and maintaining homeostasis were all reviewed. Related Articles | Metrics

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Research Progress of Prevention and Treatment on Complications of Breast Augmentation with Autologous Fat Grafting SHENG Lingling,CAO Weigang 2019, 15 (1):  52-54.  DOI: 10.3969/j.issn.1673-0364.2019.01.015 Abstract ( 340 )   Autologous fat grafting(AFG)has been applied in breast augmentation widely in recent years.With the development of basic research related to high-volume AFG and its widely clinical application in breast augmentation,it has been comprehensively cognized to prevent and treat the relative postoperative complications of breast augmentation with AFG,which were reviewed in this paper. Related Articles | Metrics

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Evaluation of Velopharyngeal Insufficiency after Cleft Palate Surgery ZHAO Jiuli,WU Di,YIN Ningbei 2019, 15 (1):  55-57.  DOI: 10.3969/j.issn.1673-0364.2019.01.016 Abstract ( 402 )   One of the main purposes of cleft palate repair is to achieve perfect velopharyngeal and acquire normal speech function.However,limited to the current surgical methods and the ability of the operators,many patients with cleft palate still have velopharyngeal insufficiency,which can not form enough pressure in the mouth.Nasal leak and abnormal nasal resonance lead to reduction of speech articulation.Therefore,it is necessary to evaluate the velopharyngeal closure after cleft palate surgery.Further treatment should be taken on the patients with velopharyngeal insufficiency.At present,there are many clinical evaluation methods for velopharyngeal insufficiency,which can be divided into subjective and objective methods.And objective methods include direct methods and indirect methods.In this paper,these methods were summarized,classified and compared,and the principles,methods,advantages and disadvantages of which were clarified.The research hotspots and prospects in recent years were indicated. Related Articles | Metrics

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Application Progress of Topical Anesthetics in Local Non-Surgical Treatment of Scar LIU Bingying,LIU Wei 2019, 15 (1):  58-60.  DOI: 10.3969/j.issn.1673-0364.2019.01.017 Abstract ( 394 )   Scar is tissue fibrotic disease caused by trauma.In addition to surgical treatment of scar,local non-surgical treatments such as laser,drug injection and cryotherapy are also important components in the treatment of scar.Pain is the most common adverse reaction in these treatments,not only affecting the therapeutic effect,but also giving the patient a certain burden on the body and mind during the treatment.In recent years,the development and application of surface anesthetics have provided an effective means to solve this problem.In this paper,the types of topical anesthetics,pharmacology,application methods,advantages and adverse reactions,as well as potential applications in non-surgical treatment of scar were reviewed and prospected. Related Articles | Metrics

Teaching

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Establishment and Implementation of the Teaching Mode of Multidisciplinary Joint Diagnosis and Treatment in Hand Surgery WANG Bin,SUN Bin,LIU Xinmeng,HAN Gang,GUO Ruiji,CUI Hengqing,FANG Xia,SUN Wenhai 2019, 15 (1):  61-62.  DOI: 10.3969/j.issn.1673-0364.2019.01.018 Abstract ( 357 )   In congenital malformations of the hand and upper limbs,about 1%of them have multiple malformations with complex pathogenesis and diverse manifestations.Usually a single department is difficult to complete the complete diagnosis and treatment of disease and research.Multidisciplinary joint diagnosis and treatment(MDT)not only brings hope for the diagnosis and treatment of these patients,but also introduces a new model for the teaching of hand surgery.In this paper,the establishment and implementation of the teaching mode of multidisciplinary joint diagnosis and treatment in hand surgery were introduced. Related Articles | Metrics