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Effects of Recombinant Periostin on Proliferation and Osteogenic Differentiation of Cranial Suture Cells in TWIST1+/- Mouse
BAI Shanshan,LI Junxian,LI Dong,XU Liang,DUAN Huichuan,YU Zheyuan,YUAN Jie,WEI Min
2017, 13 (5):
283-286.
DOI: 10.3969/j.issn.1673-0364.2017.05.012
Objective To observe the effects of recombinant Periostin on proliferation and osteogenic differentiation of cranial suture cells of coronal suture in TWIST1+/-mouse. Methods Cranial suture cells were separated and extracted from coronal suture of TWIST1+/-mouse. The cells were divided into 4 groups according to different concentration of recombinant Periostin: 0 μg/L, 50 μg/L, 100 μg/L, 200 μg/L group. CCK-8 method was used to detect the cell proliferation of cranial suture cells; Alkaline Phosphatase kit was used to detect the ALP activity; Real-Time PCR and Western Blot were used to detect the expression of osteogenic differentiation markers: OCN, OPN, BSP and COL-1. Results CCK-8 test showed that, compared with 0 μg/L group, other Periostin groups inhibited cranial suture cell proliferation in some degree (P<0.05). ALP activity test showed that, after osteoinduction for 10 days, compared with 0 μg/L group, ALP activity of 50 μg/L, 100 μg/L, 200 μg/L Periostin groups were significantly decreased (P<0.05). Real-Time PCR and Western Blot showed that, compared with 0 μg/L group, other groups significantly decreased the expression of OCN, OPN, BSP and COL-1 (P<0.05). Conclusion Different concentrations (50 μg/L, 100μg/L, 200μg/L) of recombinant Periostin can inhibit the proliferation and osteogenic differentiation of cranial suture cells of coronal suture in TWIST1+/-Mouse.
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