|
|
The Study of Combination of Human Adipose-Derived Stem Cells with Collagen Scaffold
XU Mingxi,ZHOU Zhe,ZHANG Ming,WU Jiasheng,LI Wei,SUN Kang,WANG Zhong,ZHAO Yang,LU Mujun
2012, 8 (4):
189-194.
DOI: 10.3969/j.issn.1673-0364.2012.04.003
Objective To observe the growth of human adipose derived stem cells co-culture with collagen biomaterials in vitro and in vivo.Methods hADSCs were isolated from human subcutaneous adipose tissue after collagenase digesting,filtrating and centrifuging.Then the 3 passage of hADSCs were seeded onto the collagen scaffolds and the growth of hADSCs co-culture with collagen biomaterials were observed by using Haematoxylin-Eosin staining,meanwhile the cells in the scaffolds cultured in vivo were detected by immunohistochemistry.Results Primary cultured hADSCs were spindle-shaped or fibroblasts-liked cells,and it could form the clone groups.Immunofluorescence identification of Vimentin was positive,and Flow cytometry results showed that 3 passage of ADSCs were positive for CD29,CD44,CD105,but negative for CD34,CD45.The histological study found that after cultured both in vitro and in vivo,hADSCs could grow in the space of the collagen scaffolds,moreover there were more cells in the scaffolds cultured in vivo than in vitro.When cultured in vitro,a few cells adhered at the edge of the collage 1 week later and more cells grew into the inside of the scaffolds after 2 weeks.When cultured in vivo,several cells were found at the edge of the scaffolds and parts of cells had grown into the inside even the whole layer of the scaffolds after 2 weeks,at the 4th week,a great deal of cells grew into the whole layer of the scaffolds.Simultaneously,immunohistochemistry results suggested the cells in the scaffolds’ inside cultured in vivo were from hADSCs.Conclusion Collagen scaffolds have a good biocompatibility with hADSCs,which can be used as a vehicle for hADSCs to repair tissue defect.
Related Articles |
Metrics
|
