|
Experimental Study on the Effect of Extracts of Akermanite on Proliferation and Osteo-Differentiation of Human Adipose-Derived Stem Cells
GU Huijie,GONG Lunli,ZHANG Yun,YIN Shuo,CHANG Jiang,CUI Lei
2010, 6 (6):
301-305.
DOI: 10.3969/j.issn.1673-0364.2010.06.001
Objective To elucidate the underlying mechanism of the osteo-differentiation of akermanite on human adipose-derived stem cells(hADSCs) via observing the effect of different diluted rate extracts on the proliferation and osteo-differentiation of hDASCs.Methods The original extract was prepared according to the ISO/EN 10993-5 and hDASCs were cultured in the different diluted rate extracts(1/2,1/4,1/8,1/16,1/32).The MTT assay was carried out to quantitatively evaluate the proliferation of the cells.Under the condition of osteo-induction,osteogenic differentiation of hADSCs cultured in the different extracts was detected by ALP expression and calcium deposition.Results The 1/2,1/4,1/8 extracts could concentration-dependently suppress the proliferation of hASCs,while the 1/4,1/8,1/16 extracts could promote the osteogenic differentiation of hADSCs.Among them,the 1/4 extract with the concentration of Ca2+,Mg2+ and Si4+ were 2.36 mM,1.11 mM,1.03 mM,has the optimum one.Conclusion The ions in the extract of akermanite can suppress the proliferation of hADSCs while promoting the osteogenic differentiation of hADSCs in a suitable concentration.
Related Articles |
Metrics
|