肿瘤高甲基化基因1重要下游基因HMMR对乳腺癌细胞株MDA-MB-231迁移和侵袭能力影响的体外实验

doi:10.16150/j.1671-2870.2018.01.013

摘要/Abstract

摘要： 目的：探讨肿瘤高甲基化基因1（hypermethylated in cancer 1,HIC-1）与透明质酸介导的细胞游走受体（hyaluronan-mediated motility receptor,HMMR）基因之间的调控关系,观察HMMR产物对MDA-MB-231乳腺癌细胞株的迁移和侵袭能力的影响。方法：MDA-MB-231细胞分别转染双链小激活RNA（small activating RNA,saRNA） HIC-1和双链小干扰RNA（small interfering RNA,siRNA） HMMR,通过反转录聚合酶链反应（reverse transcription polymerase chain reaction,RT-PCR）和蛋白印迹法检测观察转染后HIC-1、HMMR的mRNA和蛋白的表达情况,用CCK-8细胞增殖实验检测HIC-1和HMMR表达改变对乳腺癌细胞增殖能力的影响,再用Transwell小室观察其改变对乳腺癌细胞迁移和侵袭能力的影响。结果：转染saRNA HIC-1时MDA-MB-231细胞中HIC-1上调,同时HMMR表达下调,细胞的迁移和侵袭能力受抑制,细胞增殖能力未发生改变;转染siRNA HMMR时,MDA-MB-231细胞中HMMR表达下调,细胞的迁移和侵袭能力受抑制,细胞增殖能力未受影响。结论：HIC-1可调控HMMR的表达,HIC-1表达上调可下调HMMR,乳腺癌细胞的迁移和侵袭能力受抑制;乳腺癌细胞中因HIC-1失活,导致HMMR呈高表达,促进乳腺癌细胞的迁移和侵袭。

关键词: 乳腺癌, 高甲基化基因1, 透明质酸介导的细胞游走受体, MDA-MB-231细胞株

Abstract: Objective: To investigate the regulatory relationship between hypermethylated in cancer 1 (HIC-1) and hyaluronan-mediated motility receptor (HMMR) and to study the effect of HMMR on migration and invasion of MDA-MB-231 breast cancer cell strains. Methods: MDA-MB-231 cells were transfected with double-strand saRNA HIC-1 and double-strand siRNA HMMR, respectively. The expression of HIC-1 and HMMR in MDA-MB-231 cells were detected by RT-PCR and Western blotting. CCK-8 assay was used to detect cell proliferation after siRNA or saRNA transfection. Transwell assay was used to determine the effect of siRNA or saRNA transfection on migration and invasion of MDA-MB-231 breast cancer cells. Results: MDA-MB-231 breast cancer cells were successfully transfected. The up-regulation of HIC-1 induced HMMR down-regulation, and significantly inhibited cell migration and invasion of MDA-MB-231 breast cancer cells. HMMR was down-regulated by siRNA HMMR transfection leading to inhibition of cell migration and invasion. However, cell proliferation have not been affected in both saRNA HIC-1 and saRNA HMMR transfections. Conclusions: HMMR is regulated by HIC-1 and it plays an important role in cell migration and invasion. Inhibition of HIC-1 upregulates HMMR, thus promotes cell migration and invasion of breast cancer cells.

Key words: Breast cancer, Hypermethylated in cancer 1, Hyaluronan-mediated motility receptor, MDA-MB-231 cell strains

中图分类号:

R737.9

裴文江, 赵峰, 吴衍, 钟明, 高航, 李幼生, 顾岩, 郭善禹, 戴谦诚, 张伟. 肿瘤高甲基化基因1重要下游基因HMMR对乳腺癌细胞株MDA-MB-231迁移和侵袭能力影响的体外实验[J]. 诊断学理论与实践, 2018, 17(01): 70-75.

PEI Wenjiang, ZHAO Feng, WU Yan, ZHONG Ming, GAO Hang, LI Yousheng, GU Yan, GUO Shanyu, DAI Qiancheng, ZHANG Wei. Effect of hypermethylated in cancer 1-downstream-regulated gene HMMR on cell migration and invasion in breast cancer: an in vitro study on breast cancer cell line MDA-MB-231[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(01): 70-75.

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