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20 October 2019, Volume 15 Issue 5 Previous Issue    Next Issue

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CSSVA Guidelines for Vascular Anomalies 2019 2019, 15 (5):  277-317.  DOI: 10.3969/j.issn.1673-0364.2019.05.001 Abstract ( 2029 )   Related Articles | Metrics

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Repair of Articular Cartilage Defects Using Tissue Engineered Cartilage Constructed by Pig’s BMSCs in Vitro SONG Nan,HE Aijuan 2019, 15 (5):  318-334.  DOI: 10.3969/j.issn.1673-0364.2019.05.002 Abstract ( 410 )   Objective To investigate the feasibility of bone marrow mesenchymal stem cells(BMSCs) in vitro cartilage construction and transplantation for repairing large articular cartilage defects in large animals. Methods The cartilage defect model was established by using 7 changfeng hybrid pigs as animal models, and the cartilage defect was repaired by tissue engineered cartilage transplantation with autologous BMSCs in vitro targeted induction for 8 weeks. The pure PGA/PLA material group, the blank control group and the normal cartilage group were set up as control. Six months after operation,gross and histological examinations were carried out. At the same time, the expression levels of the key cytokines such as IGF-1, IL-1β, MMP-13 and TNF-α in synovial fluid were detected before and after operation. The repair effect was evaluated comprehensively. Results Six months after the operation, most of the experimental groups obtained ideal cartilage tissue repair, the general view and histological maturity of the cartilage tissue in the repair area was very close to the normal cartilage tissue. In the test of joint fluid, the expression of IGF-1 in the experimental group was significantly higher than that in the blank control group and PGA group(P <0.05), while the expression levels of IL-1β, MMP-13 and TNF-α were significantly lower than those in the blank control group and PGA group(P<0.05). Conclusion Tissue engineered cartilage constructed by pig’s BMSCs in vitro can effectively repair articular cartilage defects, thus avoiding or delaying the development of osteoarthritis. Related Articles | Metrics

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Experimental Study on the Osteogenic Induction by Transwell Co-Culture of Rat BMSCs and ADSCs in Vitro JIN Xin,ZHANG Zhiliang,LU Yi,HUANG Yixiong,FAN Zhihong,CHEN Rui 2019, 15 (5):  322-324.  DOI: 10.3969/j.issn.1673-0364.2019.05.003 Abstract ( 535 )   Objective To study the effect of Transwell co-culture of rat BMSCs and ADSCs on the osteogenic differentiation of ADSCs in vitro. Methods ADSCs and BMSCs were obtained from the same 6-week-old SD male rats. The second passage of ADSCs and BMSCs were harvested and placed into Transwell co-culture system at a ratio of 1 ∶1(co-culture group), ADSCs were placed in the bottom and BMSCs in the upper. Meanwhile ADSCs group and BMSCs group were served as control group. After 21 days of osteogenic induction and differentiation, the qualitative and semi-quantitative staining analysis of alizarin red were used to investigate the effect of BMSCs on ADSCs osteogenic differentiation. Results The results of semi-quantitative determination of alizarin red in the co-culture group were statistically different from that in the ADSCs group(P0.05). Conclusion Osteogenic mineralization ability was enhanced after in vitro Transwell co-culture of BMSCs and ADSCs, and BMSCs could promote osteogenic differentiation of ADSCs by paracrine. Related Articles | Metrics

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Effect of mi R-2053 on Fibroblasts in Oral Mucosal Repair and Its Mechanism YU Meichan,CHEN Kaiyan,LIU Siyuan 2019, 15 (5):  325-329.  DOI: 10.3969/j.issn.1673-0364.2019.05.004 Abstract ( 364 )   Objective To explore the effect of miR-2053 on fibroblasts in oral mucosal repair and its mechanism.Methods Forty patients with oral mucosal disease were selected as the case group, and 40 healthy subjects from the physical examination center were selected as the control group. Real-time quantitative PCR was used to verify the expression of miR-2053 in oral mucosa. The human gingival fibroblasts were transfected with miR-2053. The cell viability was detected by CCK-8 method. The cell mobilization rate was detected by flow cytometry and the cell migration rate was detected by Transwell chamber method. RT-PCR and Western-blot were used to detect the key transcription factors Collagen Ⅰ,Collagen Ⅲ, MMP-1, MMP-13 and their protein expression levels in tissue remodeling. Results Compared with the control group, the expression of miR-2053 in oral mucosa was significantly increased(P<0.05). Cell viability and migration rate of human gingival fibroblast model transfected with miR-2053 were both significantly lower than that of the control group and the negative analogue group(P <0.05), and the apoptosis was significantly increased(P <0.05). The expression levels of Collagen Ⅰ, Collagen Ⅲ, MMP-1 and MMP-13 in human gingival fibroblasts transfected with miR-2053 were significantly decreased compared with the control group and the negative analogue group(P <0.05). Further detection of protein levels revealed that the relative expression levels of Collagen Ⅰ, Collagen Ⅲ and MMP-13 in human gingival fibroblasts transfected with miR-2053 were significantly lower than those in the control group and the negative analogue group(P<0.05).Conclusion miR-2053 may affect the vitality, apoptosis and migration of human gingival fibroblasts by affecting the expression of Collagen Ⅰ, Collagen Ⅲ, MMP-1 and MMP-13, thus affect the rehabilitation of oral mucosal disease and the disease course. Related Articles | Metrics

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Breast Implant Augmentation Via Endoscopic Assisted Axillary Approach: a Long-Term Retrospective Study in 53 Cases AN Juan,YU Li,LIN Huaian,ZHENG Danning,ZHANG Bo,WANG Jian,ZHOU Jia,ZHANG Yifan 2019, 15 (5):  330-334.  DOI: 10.3969/j.issn.1673-0364.2019.05.005 Abstract ( 339 )   Objective To evaluate the long-term effects of breast implant augmentation via endoscopic assisted axillary approach. Methods A retrospective analysis was conducted on 53 patients who underwent endoscopic assisted transaxillary double dual plane breast implant augmentation from July 2014 to December 2016. All patients were followed up for more than 18 months. Postoperative breast diameters were measured, the complications related to prosthesis augmentation was evaluated, and the postoperative satisfaction of patients was evaluated. Results The post-operative length of the suprasternal notch to nipple, clavicle midpoint-to-nipple distance and nipple-level chest circumference were significantly increased compared with the preoperative one(P 0.05). A total of 29 cases suffered complications after operations, of which the vast majority(98%) had mild complications(such as nipple sensory changes, the implant edge palpability), and only 1 patient(2%) had severe complications(such as Ⅲ-Ⅳ capsule contracture). Conclusion Endoscopic assisted transaxillary double dual plane breast implant augmentation is clearly able to increase the breast volume of patients with fewer complications, which is worthy of clinical application. Related Articles | Metrics

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Intense Pulsed Light Combined with Fractional Er:YAG Laser in the Treatment of Post-Traumatic Scar in Children CHEN Kun,WANG Yining,WANG Yanni,HU Nan,LI Dan,QI Hongyan 2019, 15 (5):  335-348.  DOI: 10.3969/j.issn.1673-0364.2019.05.006 Abstract ( 476 )   Objective To explore the clinical efficacy of intense pulsed light combined with fractional Er:YAG laser in the treatment of post-traumatic scar in children. Methods From July 2017 to June 2019, 107 children with post-traumatic scar were included in the study, with an average age of(3.97 ±3.14) years, and were all treated with intense pulsed light combined with fractional Er:YAG laser. The modified VSS and VRS were used to evaluate the clinical efficacy and the complications were recorded. Results A total of 107 children were treated by 353 laser treatments. According to the modified VSS, the score of hyperemia, thickness, flexibility and total score decreased significantly after treatment(P<0.05).The VRS score before scar treatment was 1.55±1.16, and after scar treatment was 0.86±0.93, which was significantly lower(P <0.05). There were 16 cases of adverse reactions after treatment. Conclusion Intense pulsed light combined with fractional Er:YAG laser in the treatment of post-traumatic scar in children has obvious curative effect and high safety, which is worthy of clinical application. Related Articles | Metrics

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Evaluation of Sensory Recovery of the Reverse Island Flap Based on the Digital Artery Distal Dorsal Branch for Fingertip Repair CAO Yi,DU Zijing,HAN Dong,JIANG Hao 2019, 15 (5):  338-341.  DOI: 10.3969/j.issn.1673-0364.2019.05.007 Abstract ( 483 )   Objective To evaluate the sensory recovery of both innervated and noninnervated homodigital island flaps based on the digital artery distal dorsal branch(DDB) for fingertip repair. Methods Twenty-seven cases of fingertip injuries repaired with DDB flaps were retrospectively investigated. Mean follow-up time was 13.2 months(6-28 months). Sensory recovery was assessed using SW monofilament, static and moving two-point discrimination tests. Pain and cold intolerance were respectively measured by visual analogue scale and Cold Intolerance Severity Score questionnaire. Results All flaps survived. Patients were satisfied with the fingertip appearance. Flap sensation had no significant difference in both innervated and noninnervated groups according to long-term follow-up. Conclusion The digital artery DDB flap is an alternative method for fingertip repair. Noninnervated DDB flap can restore flap sensation in long-term evaluation. Related Articles | Metrics

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Application of Nasolabial Flap in the Treatment of Large Nasal Skin Mass HOU Junjie,LIU Chang,CHEN Fengchao,ZHANG Siya,YAN Tongtong,JIA Yulei 2019, 15 (5):  342-344.  DOI: 10.3969/j.issn.1673-0364.2019.05.008 Abstract ( 460 )   Objective To explore the application of nasolabial flap in the repair of skin defect after the removal of large nasal mass. Methods From October 2015 to January 2019, twenty-two patients with large nasal skin mass were treated with nasolabial flap for wound repair after tumor resection. The repair efficacy, incision scar and postoperative morphology of nasal alar were retrospectively analyzed. Results All the nasal lesions were completely excised and no recurrence was observed.The appearance of the skin flap was satisfactory after repair, and the scar at cutting edge and donor area was not obvious.Good appearance of nasal alar was observed. Conclusion The nasolabial flap is a good method to repair the defect of the larger nasal skin mass after excision. According to the size and location of the lesion, different methods of flap transfer can be used. Related Articles | Metrics

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Internal Fixation with PFNA and DHS +Cannulated Screw on Intertrochanteric Fractures in the Elderly ZHENG Yong,SHI Fajian,ZHAO Xiaolong,WANG Fang 2019, 15 (5):  345-348.  DOI: 10.3969/j.issn.1673-0364.2019.05.009 Abstract ( 399 )   Objective To compare the efficacy of internal fixation with PFNA and DHS +cannulated screw in the treatment of intertrochanteric fractures in the elderly. Methods A retrospective study was conducted to enroll patients with intertrochanteric fractures in the elderly from January 2014 to March 2018. Finally, 64 patients were included, including 24 patients treated with PFNA fixation(PFNA group), and 40 patients treated with DHS combined with cannulated screw(DHS+cannulated screw group). The duration of surgery, intraoperative blood loss, postoperative complications, fracture healing time and the last recorded hip Harris score were recorded. Results Compared with DHS+cannulated screw group, PFNA group had shorter duration of surgery, less intraoperative blood loss and lower postoperative complications(P <0.01). Moreover,fracture healing in the PFNA group was faster than that in the DHS +cannulated screw group, and the last recorded Harris score was higher(P<0.01). Conclusion For elderly patients with intertrochanteric fractures, internal fixation with PFNA has better efficacy and fewer complications than internal fixation with DHS combined with cannulated screw, and is worthy of clinical promotion. Related Articles | Metrics

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Efficacy of Dexmedetomidine Combined with Dezocine Assisted Ultrasound-guided Brachial Plexus Block Anesthesia in the Treatment of Upper Limb Fracture Surgery SUN Mei,GU Shixian,WANG Qingbao,ZENG Rong,MAO Gu,GUO Rongchun,LIU Juan,GONG Xiaoyi,CAO Zhi,TAO Xiuting 2019, 15 (5):  349-355.  DOI: 10.3969/j.issn.1673-0364.2019.05.010 Abstract ( 438 )   Objective To observe the efficacy of dexmedetomidine combined with dezocine-assisted ultrasound-guided brachial plexus block anesthesia in the treatment of upper limb fracture surgery. Methods A total of 60 patients with upper limb fracture were enrolled in this study, who were treated from January 2017 to March 2019. All the patients were randomly divided into the experimental group and the control group, with 30 cases in each group. Patients in the experimental group were anesthetized with dexmedetomidine combined with dezocine for ultrasound-guided brachial plexus block, while patients in the control group were anesthetized with dezocine for ultrasound-guided brachial plexus block. Blood pressure, heart rate,oxygen saturation and Ramsay sedation scores were compared between the two groups during the perioperative period. The incidence of intraoperative adverse reactions and the VAS scores after operation were compared between the two groups.Results The oxygen saturation of the two groups were stable at each time point during the perioperative period with no significant difference(P>0.05). The blood pressure and heart rate of the experimental group were stable at each time point during the perioperative period with no significant difference(P >0.05). The blood pressure and heart rate fluctuated significantly in the control group during the perioperative period(P 0.05). Ramsay sedation scores immediately after nerve block,10 minutes after anesthesia, 5 minutes after operation, 30 minutes after operation and at the end of operation in the experimental group were all significantly higher than those in the control group with statistical significance(P0.05), and there were no serious adverse reactions in both groups. Postoperative VAS score showed that the VAS of the experimental group and the control group were(1.6 ±0.5) and(2.0 ±0.6) respectively, with no statistical significance(P >0.05). Conclusion Dexmedetomidine combined with Dizosin assisted ultrasound-guided brachial plexus block anesthe sia is safe and effective with better sedative and analgesic effect in the treatment of upper limb fracture surgery. Related Articles | Metrics

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Evaluation of Node Management in Reducing the Loss Rate of Orthodontic Articles in Orthognathic Surgery YING Yuanjie,YANG Xu,ZHANG Yong,YUAN Hao,YANG Yuelai,ZHANG Shilei 2019, 15 (5):  352-355.  DOI: 10.3969/j.issn.1673-0364.2019.05.011 Abstract ( 449 )   Objective To in vestigate the main causes for the loss of orthodontic items in orthognathic surgery, and to explore the effect of node management in reducing the loss rate of orthodontic articles. Methods A research team was set up to analyze the causes of the loss of orthodontic items during operation, and to explore the possible surgical procedures so as to develop control measures. From June 2018 to January 2019, 763 patients undergoing orthognathic surgery were selected,all of whom were wearing orthodontic items in their mouths. Among them, 372 cases undergoing orthognathic surgery from June to September 2018 were set as the control group, which adopted the traditional counting process. Three hundred and ninety-one patients undergoing orthognathic surgery from October to January 2019 were set as experimental group and node management was adopted. The loss rate of orthodontic items and work efficiency before and after intervention were compared.Results After the application of node management, the loss rate of orthodontic items in the orthognathic surgery were significantly reduced, and the ineffective anesthesia and operation time were shortened. The differences were all statistically significant(P<0.05). Conclusion The application of node management can effectively reduce the loss rate of orthodontic items in orthognathic surgery, promote the smooth operation of orthognathic surgery, and ensure the safety of patients. Related Articles | Metrics

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The Influence of Family Members’ Synchronous Instruction on Uncertainty in Illness Among Patients with Postoperative Limb Lymphedema Treated with Complex Decongestion Therapy WANG Hairong,YANG Jiafei,BIAN Weiwei,LU Wei 2019, 15 (5):  356-358.  DOI: 10.3969/j.issn.1673-0364.2019.05.012 Abstract ( 373 )   Objective To evaluate the influence of family members’ synchronous instruction education on uncertainty in illness among patients with postoperative limb lymphedema(PLL) treated with complex decongestion therapy(CDT). Methods From February 2018 to March 2018, convenience sampling was adopted to investigate 60 patients with PLL treated with CDT. The 60 patients were divided into control group and experimental group averagely and randomly. The 30 patients in the control group received routine health education, and the patients in the experimental group received routine health education and synchronous instruction for family members together. The patients in the two groups were investigated by Mishel disease uncertainty scale(Chinese version) before CDT treatment and after one course of CDT treatment. Results Before the first CDT treatment, the disease uncertainty scores of the control group and the experimental group were(70.60 ±4.78) and(69.67±3.72),respectively, with no significant difference between the two groups(P>0.05); after one course of CDT treatment, the disease uncertainty scores of the control group and the experimental group were(67.10 ±3.19) and(59.40±2.28), respectively, with statistical difference. Significance(P<0.05). Conclusion The uncertainty in illness for the PLL patients treated with CDT can be effectively reduced by the combination of routine health education and synchronous instruction for family members. Related Articles | Metrics

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Research Progress of Bio-3D Printing for the Repair of Cartilage Damage HAN Qianqian,WANG Miaomiao,SHI Jianfeng,WANG Ying,LIAN Huan,WANG Rui,LI Jingli,WANG Chunren 2019, 15 (5):  359-361.  DOI: 10.3969/j.issn.1673-0364.2019.05.013 Abstract ( 386 )   The self-repairing ability of cartilage tissue is limited due to the lack of blood supply. This makes it difficult to regenerate and repair after cartilage injury. The bio-3 d printing technology breaks through the limitations of bionic function design of traditional tissue engineering technology, and can be integrated into molding and accurately prepare complex structures. Moreover, it can also print cells into scaffold materials to construct microenvironment suitable for cell survival, which has advantages in the field of cartilage repair and regeneration. In this paper, the research progress of the cartilage repair using bio-3 D printing was reviewed. Related Articles | Metrics

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Research Progress of Tissue Engineering Myocardial Patch TAN Yao,FU Wei 2019, 15 (5):  362-367.  DOI: 10.3969/j.issn.1673-0364.2019.05.014 Abstract ( 481 )   Heart failure caused by myocardial infarction is the main cardiovascular disease that threatens human health.Tissue engineering myocardial patch prepared by stem cell combined with scaffold material is expected to become the hope of myocardial infarction treatment in the near future. At present, there are many researches on tissue engineering myocardial patches. The cells and scaffolds are diverse. In this paper, the various cell sources and scaffolds that may be used and various factors that influence the survival and function of tissue engineered myocardial patches were all reviewed. Related Articles | Metrics

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Research Progress of Microspheres in Bone Tissue Engineering CUI Yunqian,TAN Yu,WU Yiqun 2019, 15 (5):  368-372.  DOI: 10.3969/j.issn.1673-0364.2019.05.015 Abstract ( 409 )   Microspheres refer to the tiny spherical particles made from natural or synthetic polymers, whose diameters range from tens of microns to hundreds of microns.This material serves as an a bone tissue engineering material with great application potential due to its good porosity, biocompatibility, biodegradability, delivery and sustained release of biomolecules and osteoinductive properties. In the process of bone regeneration, microspheres can provide a microenvironment for the adhesion, proliferation and differentiation of seed cells, maintaining a certain three-dimensional configuration and guiding the migration and growth of tissue cells. In addition, microspheres can be used as a carrier of stem cells, growth factors and drugs to better regulate the formation of new bone. In this paper, the characteristics of microspheres and the research progress in bone tissue engineering were reviewed and the future application and development of microspheres were prospected. Related Articles | Metrics