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23 July 2020, Volume 11 Issue 4 Previous Issue    Next Issue

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Chondrogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells Induced by Sox9 ZHANG Jun,ZHANG Yaoming,WANG Zhenghui,ZHANG Qing,LUO Huanan,ZHENG Guoxi,HOU Jin,WANG Botao,YANG Yeye,ZHAO Xiaoyan,SHI Yanxia 2015, 11 (4):  221-224.  DOI: 10.3969/j.issn.1673-0364.2015.04.001 Abstract ( 499 )   Objective To investigate the effect of Sox9 gene on inducing the chondrogenic differentiation of human umbilical cord mesenchymal stem cells. Methods Human umbilical cord mesenchymal stem cells (hUCMSCs) were obtained by microdissection and digestion, and cultured in monolayer. The growth velocity and morphological changes of hUCMSCs were observed after Sox9 transfection. The expression of collagen Ⅱ and aggrecan were detected by immunohistochemistry. The mRNA expression of collagen Ⅰ, collagen Ⅱ and aggrecan were detected by RT-PCR, and collagen Ⅱ content was detected by western blot. Results The Sox9 gene had no significant effect on the morphology of hUCMSCs, and significantly accelerated the differentiation of hUCMSCs to chondrocytes compared with the traditional induction group. Conclusion Sox9 gene can regulate the differentiation of hUCMSCs, and hUCMSCs can be used as seed cells for cartilage tissue engineering. Related Articles | Metrics

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Construction of Tubulous Cartilage with Chondrocyte-sheets and 3D Printed Supporter GE Yang,HAN Lu,FENG Bei,BAI Jie,HE Xiaomin,YIN Meng,WANG Wei,XU Zhiwei,FU Wei,LU Yanan 2015, 11 (4):  225-233.  DOI: 10.3969/j.issn.1673-0364.2015.04.002 Abstract ( 450 )   Objective To construct tubulous cartilage by porcine auricular cartilage-derived chondrocyte-sheets combing with 3D printed supporter for repairing tracheal cartilage defects. Methods Primary chondrocytes were isolated from porcine auricular cartilage with methods of trypsin enzyme digestion and collagenase enzyme digestion. After proliferation in vitro, the chondrocytes of passage 2 were seeded on culture dish with a high density of 5 ×105 cells/mL, and intact chondrocyte-sheets were detached after cultured in a specific cell sheet culture environment for 14 days in vitro. Four detached chondrocyte-sheets were rolled onto a 3D printed supporter of PA material one by one to construct a tubulous cartilage-like complex. Twelve weeks’ static culture in vitro as well as 8 weeks' subcutaneous culture in nude mouse were performed. Gross inspection and paraffin section staining were applied for evaluation. Results Both in-vitro and in-vivo culture contributed to formation of tubulous cartilage with specific mechanical property. The cartilage extracellular matrix secretion and mechanical property of tissue engineered tubulous cartilage tissue cultured in vivo were better than that cultured in vitro. Conclusion It is feasible to construct tissue engineered tubulous cartilage tissue by chondrocyte-sheets and 3D printed PA supporter for tracheal cartilage repair. Related Articles | Metrics

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The Biocompatibility of Zinc-modified CaSiO3 Bioceramic Coating in vitro XU Lizhang,YU Jiangming,LI Kai,ZHENG Xuebin,DENG Guoying,WANG Weiheng,HUANG Xiaodong,YE Xiaojian 2015, 11 (4):  229-233.  DOI: 10.3969/j.issn.1673-0364.2015.04.003 Abstract ( 501 )   Objective To study the biocompatibility of zinc-modified CaSiO3 bioceramic coating in vitro, and to provide experimental basis of its clinical application. Methods The physical characteristic of the coating and the concentration of differentions extracted from the coating were detected. Then the extract was used in MC3T3-E1 cells culture with different concentrations for cellular morphological observation, including the relative growth rate measured by MTT and the apoptosis rates detected by flow cytometry. Hemolysis ratio of the coating was also detected. Results Calcium, Silicon, Zinc ion concentrations of the coating's extract were significantly increased after immersion for 72 hours. All cells cultured with different proportions of diluent showed normal cellular morphology. Cells cultured in group 3.125% and 6.25% had larger quantity, higher relative increment rate and lower apoptosis rate, compared to positive control group (complete medium). Alone with the increasing concentrations, mild inhibition to the cells and increasing apoptosis rate were observed. The hemolysis rate of the coating's extract detected by hemolysis test was 0.5%. Conclusion The Zinc-modified CaSiO3 bioceramic coating with non-cytotoxicity and good biocompatibility has the biological basis of surface modification for bone implants and other biological material. Related Articles | Metrics

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Maintenance of Cell Surface Marker CD105 and Its Function by Hanging-drop Culture Method CAO Xin,XIAO Ran,FU Xin,YAN Li 2015, 11 (4):  234-238.  DOI: 10.3969/j.issn.1673-0364.2015.04.004 Abstract ( 757 )   Objective To investigate the maintenance of cell surface markers of keloid fibroblasts by hanging-drop culture method, and to preliminarily study the role of CD105 in regulating function of cells. Methods The fibroblasts were isolated from 3 keloid samples of ears, and the passage 5 cells were cultured using the adherent culture and the hanging-drop culture method for 1 week respectively. The passage 4 (P4) and 5 cells (P5) cultured by adherent culture method and the passage 5 cells cultured by hanging-drop culture method (P5HD)from the same patient were detected for the expression of cell surface markers including CD105, CD90, CD73 and CD44 by multicolor flow cytometry analysis. The mRNA expressions of surface markers and CTGF, Col IA1, Col IA2 were detected using real-time PCR. Results The positive cell percent of CD105+ and CD73+CD90+CD105+ in P4 and P5HD group were significantly higher than in P5 group by flow cytometry analysis, but there was no difference between P4 and P5HD group. The positive cell percent of CD73+, CD90+and CD44+ among three groups had no difference. The CD105 results of real-time PCR were in accordance with that of flow cytometry analysis. The mRNA expression tendency of CTGF and ColIA1 was similar to CD105. Conclusion The hanging-drop culture method is helpful to maintain the expression of CD105 surface marker and functional genes related to fibrosis and collagen production, then to keep the biological function of keloid fibroblasts, but the underlying mechanism needs to be discovered. Related Articles | Metrics

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The Designing of hADSCs Stacking Model by Utilizing PDMS Microwell Micro-reactor HE Yi,LUAN Jie 2015, 11 (4):  239-245.  DOI: 10.3969/j.issn.1673-0364.2015.04.005 Abstract ( 528 )   Objective To establish a three-dimensional cultivation platform for human adipose derived stem cells (hADSCs) by utilizing micro-scale technology, and to explore the influence of three-dimensional stacking condition to cell proliferation and apoptosis. Methods Micro-columnar structure were generated by micro-scale technology on silicon master with diameter of 60 μm, 80 μm, 100 μm and 150 μm respectively. The same specification micro-well arrays were acquired by reversing mould aperture using polydimethylsiloxane (PDMS), which equipped with PDMS ring on the outer wall to constitute micro-reactor. The error rate of this platform was analyzed. Then the third generation of human adipose-derived stem cells (hADSCs) were selected and mixed into single cell suspension mixture with three kinds of concentration, including 3×104 cells/mL, 6×104 cells/mL and 1×105 cells/mL. And 200μL cell suspension was injected into micro-reactor to establish three-dimensional culture models with different stacked layers. Then 24 hours, 72 hours, 120 hours and 168 hours were choosed as sample collection time point. The morphology description of cell distribution in each group was analyzed with scanning electron microscopy, the arrangement inside the microwells and the cell distribution outside the microwells were described, and the live/dead cells were counted and tested in each aperture and micro hole by using laser scanning confocal microscope. Results The error rate of diameter and pore diameter were no more than 0.2%. When hADSCs were planted by the density of 6 ×104 cells/mL, the stacking layer condition can be acquired. The number of cell within microwells was at static condition no more than 7 days. The 60 μm group had the highest number of out-of-well cell (P<0.05) and lowest 05). The highest apoptosis ratio was also observed in the 60 μm group (P<0.05). Conclusion The application of microscale technique can acquire extremely low error rate, which can satisfy the requirements of high precision design. The static proliferation condition of planted hADSCs can be maintained within microwells, which can stabilize the condition of cells and be designed as a perfect three dimention model for cell research at early stage of plant. The stackable condition of hADSCs can be acquired by planting into microwells with different diameters, which can significant affect the rate of apoptosis under this three dimentional cultivate condition. Thinner layer of stack may reduce the rate of apoptosis. Related Articles | Metrics

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The Long-term Research of Propranolol Treatment for Giant Parotid Infantile Hemangomas JIN Yunbo,CHANG Lei,ZOU Yun,LV Dongze,QIU Yajing,YING Hanru,CHEN Bin,MA Gang,CHEN Hui,LIN Xiaoxi 2015, 11 (4):  246-254.  DOI: 10.3969/j.issn.1673-0364.2015.04.006 Abstract ( 466 )   Objective To evaluate the long-term efficacy of propranolol for giant parotid infantile hemangiomas. Method From March 2009 to October 2009, 11 cases of giant parotid hemangiomas were treated with propranolol orally (2 mg/Kg·d). When children aged 8-12 months, propranolol treatment was stopped and the long-term efficacy was observed. Results More than 5 years follow-up had been completed in each patient (mean 69.5 ±4.8 months). Excellent results were observed in 10 cases (90.9%). Among them, a little skin laxity and red spot were residual in 6 cases, and further therapy was necessary to improve appearance. One case (9.1%) still had significant lump and erythema at 5 years follow-up. Conclusion The long-term efficacy of propranolol in treating gaint parotid infantile hemangiomas is not good enough. Other therapy is still necessary for the residual mass and skin lesion. Related Articles | Metrics

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Preliminary Study of Selected Temporal PNF Resection HU Xiaojie,GAO Yang,CHEN Hui,LIN Xiaoxi,LI Wei,WANG Wei,MA Gang,JIN Yunbo,WANG Tianyou,QIU Yajing,YANG Xi,CHANG Lei 2015, 11 (4):  248-251.  DOI: 10.3969/j.issn.1673-0364.2015.04.007 Abstract ( 505 )   Objective To explore the application of selective PNF resection in protecting the temporal branch of facial nerve. Methods From November 2011 to December 2012, 14 patients with PNF of the temporal area were operated. 9 patients were performed unselective tumor removal and 5 patients were performed selective tumor removal. The results were observed and compered between the two groups. Results All patients got face-looking improved after operation. But the function of temporal branch of facial nerve of non-selective removal patients was damaged and the eyebrows could not be raised after operation. The functions of facial nerve of selective removal patients were preserved. All the patients were followed up for 15 to 24 months, PNF recurrence was not observed. Conclusion The function of temporal branch of facial nerve can be preserved with the PNF selective removal. Related Articles | Metrics

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Application of Levator Veli Palatini Retropositioning Combined with Buccal Fat Pad Flap for Complete Cleft Palate Repair ZHANG Hongchuang,WAN Yanjun,ZHANG Yang,ZHANG Yang,CHENG Yusheng,DU Chunrong 2015, 11 (4):  252-254.  DOI: 10.3969/j.issn.1673-0364.2015.04.008 Abstract ( 420 )   Objective To explore a method of functional palatoplasty for reducing the incidence of palatal fistula without interfering the maxillary growth. Methods From 2013, a total of 38 patients with cleft palate received levator veli palatini retropositioning combined with buccal fat pad flap. Results All patients were healed by first intention, no palatal fistula was observed. All the patients were followed up for 1-2 years, significant improvement in speech intelligibility was achieved. Conclusion Levator veli palatini retropositioning combined with buccal fat pad flap is a useful procedure for cleft palate repair with low incidence of palatal fistula and satisfying rate of velopharyngeal competence. Furthermore, this technique affects growth of maxillary very slightly and is worthy of further application. Related Articles | Metrics

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Application of Laser Hair Removal in Preparation of Expanded Perforator Scalp Flap DONG Jiying,JIN Rui,XIE Feng,ZAN Tao,WANG Shen 2015, 11 (4):  255-257.  DOI: 10.3969/j.issn.1673-0364.2015.04.009 Abstract ( 380 )   Objective To study the safety and efficacy of laser hair removal during expansion of the expanded perforator scalp flap. Methods From January 2014 to April 2015, 22 patients received expanded scalp flap preparation for reconstruction of facial soft tissue deformation were included. Diode laser therapy was used to remove hair during tissue expansion period. Results All patients completed the multiple-stage treatment:expander implantation-saline injection-laser hair removal-flap translocation. All flaps were survived after flap translocation. No thick hair was observed in 19 patients, small area of hair remaining was observed in 2 patients, and psilosis at hair edge was observed in 1 patient. Transient blister was observed in one patient after laser hair removal, which subsided without treatment. No long-term adverse events were observed. Conclusion Laser hair removal technique can be safely and effectively used for the preparation of expanded scalp flap. Improvements of facial appearance and satisfaction can be achieved. Related Articles | Metrics

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Observation on the Efficacy of Skin Grinding Combined with External Application of Recombinant Human Basic Fibroblast Growth Factor in the Treatment of Superficial Scars LI Zhiming,CHAI Yong,QIAN Niwen,HUANG Yuanfa,FANG Yunde 2015, 11 (4):  258-260.  DOI: 10.3969/j.issn.1673-0364.2015.04.010 Abstract ( 458 )   Objective To explore the clinical efficacy of skin grinding combining with external application of recombinant human basic fibroblast growth factor (rh-bFGF) in the treatment of superficial scar. Methods From February 2010 to March 2015, 23 patients with superficial scar were treated with dermabrasion, then the wound was covered with vaseline gauze that accompanied by rh-bFGF solution, and bandaged. Follow-up and curative effect evaluation were carried out after operation. Results After treatment, markedly effective results were observed in 18 cases, effective results were observed in 3 cases, ineffective results were observed in 2 cases, and the effective rate was 90.9%. All the patients were followed up for 1 to 8 months. The wound showed different degree of erythema and pigmentation after operation, and all of them obtained satisfactory results through active nursing and treatment. Conclusion Skin grinding combining with external application of rh-bFGF can reduce the time of wound healing and inhibit the formation of scar tissue, the curative effect is reliable. Related Articles | Metrics

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Combination of Autologous Dermis Flap in Rhinoplasty MA Qun,JIANG Yanan,XU Yihao 2015, 11 (4):  261-265.  DOI: 10.3969/j.issn.1673-0364.2015.04.011 Abstract ( 389 )   Objective To explore the application of autologus dermis flap combining with other materials in rhinoplasty. Methods From January 2014 to January 2015, according to the condition of the patient’s nasal, 31 cases received rhinoplasty using autologous dermis flap combining with silicon prosthesis and auricle cartilage, and the clinical effects were observed. Results The dermis flaps were survived in all the cases. Satisfactory and natural nasal shape was obtained. All the patients were followed up for 6 months to 1 year. Only 1 patient got infection after surgery and healed after anti-inflammatory. The other patients were satisfied with the outcomes without complication. Conclusion Autologous dermis flap combining with silicon prosthesis and auricle cartilage for rhinoplasty can reduce the incidence of complications, like prosthesis exposure, and achieve natural nasal shape. Related Articles | Metrics

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Factors Affecting the Functional Recovery of Proximal Humerus Fractures of Elderly People ZHAN Zufeng,LIU Xiaoxia,YAO Zhongjun,LIAO Youqiao 2015, 11 (4):  263-265.  DOI: 10.3969/j.issn.1673-0364.2015.04.012 Abstract ( 465 )   Objective To explore the impact factors of functional recovery of proximal humerus fractures in elderly people. Methods From September 2012 to March 2014, 82 elderly patients with proximal humeral fractures treated in our hospital were retrospectively analyzed. The patient's age, sex, surgical approach, fracture type, injury factors, functional exercise and perioperative complications were recorded. DASH score and Constant shoulder scoring system were set up as the standards. Univariate analysis were used to explore the impact factors of functional recovery of proximal humerus fractures in elderly people. Results According to the DASH score and the Constant-Murley score, excellent results were showed in 66 cases, good in 11 cases, fair in 4 cases, poor in 3 cases, and the excellent and good rate was 93.90%. There were no significant differences between male and female patients, and also between the locking plate fixation group and traditional internal fixation group, P>0.05; The prognosis of patients in Neer Ⅰ, Ⅱ type group, non-high-energy injury group, timely rehabilitation group and no perioperative complication group were significantly better than patients in Neer Ⅲ,Ⅳtype group, high-energy injury group, no rehabilitation group and perioperative complication group respectively (P<0.05). Conclusion Fracture type, injury factors, functional training, perioperative complications are the main factors affecting the functional recovery of proximal humerus fractures in elderly patients, while gender and surgical approach have less influence. Related Articles | Metrics

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Transplantation Studies of Pluripotent Stem Cells at Different Differentiated Stages LI Jun,DU Xiao,ZHOU Xiaonan,WU Yanshuang,LEI Lei 2015, 11 (4):  266-268.  DOI: 10.3969/j.issn.1673-0364.2015.04.013 Abstract ( 467 )   Induced pluripotent stem cells (iPSCs) derived from reprogrammed somatic cells, are totipotential cells similar to embryonic stem cells. Recently, iPSCs inducing technology has been promptly improved in methods of induction and differentiation in vitro. Consequently, iPSCs at different differentiated stages have been widely used in cell transplantation experiments. Here, the current researches of iPSCs transplantation were summarized, some novel methods comparing with the older one and some problems need to be resolved before iPSCs could be applied in clinic therapy were all reviewed. Related Articles | Metrics

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Stem Cells and the Repair of Spinal Cord Injury HAN Qianqian,XU Yan,WANG Hong,HUANG Jingchun 2015, 11 (4):  269-273.  DOI: 10.3969/j.issn.1673-0364.2015.04.014 Abstract ( 523 )   Spinal cord injury is still a disease which is hard to cure in clinic and a project under research. The operation treatment and corresponding auxiliary treatment did not achieve a satisfied result. The cell-therapy of stem cells transplants to the patients' spinal cord injury area is a promising method. By the replacement of damaged cells, reducing the formation of glial scar, promoting the survival of neurons axonal regeneration and the synapse's formation, stem cell promote the recovery of function and morphology. In thispaper, the application of stem cell therapy in treating spinal cord injury was reviewed and prospected. Related Articles | Metrics

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Angiogenesis of Adult Stem Cells Induced by Hypoxia WANG Qiong,LU Mujun 2015, 11 (4):  274-277.  DOI: 10.3969/j.issn.1673-0364.2015.04.015 Abstract ( 448 )   In recent years, tissue engineering and regenerative medicine provide a new method to treat the damaged tissues and organs, but under the pathological conditions, the low survival rate of the transplanted stem cells limits its function. Because of this, hypoxic preconditioning of the stem cells in vitro become a new solution to improve its ability to resist hypoxia and injury. In this paper, the advantages and disadvantages of different inducing conditions and different adult stem cells were reviewed to search the ideal conditions of angiogenesis. Related Articles | Metrics

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Research Progress of Navigation Technique in Soft Tissue Surgery Based on Three-dimensional Ultrasound Imaging YANG Chengshuai,ZHANG Shilei 2015, 11 (4):  278-281.  DOI: 10.3969/j.issn.1673-0364.2015.04.016 Abstract ( 521 )   With the rapid development of medical imaging technology in recent years, it can improve the surgical accurace, dexterity, safety and decrease the operative time by integrating high-quality medical images to the surgical navigation system. In this paper, the development of navigation technique in soft tissue surgery based on three-dimensional ultrasound imaging was reviewed and its technical principles, clinical applications, advantages and disadvantages were expounded systematically. Related Articles | Metrics

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Application of Autologous Platelet Concentrates in Plastic and Aesthetic Surgery GAO Yashan,LI Qingfeng 2015, 11 (4):  282-285.  DOI: 10.3969/j.issn.1673-0364.2015.04.017 Abstract ( 511 )   Autologous platelet concentrates could promote tissue healing and regeneration by the secretion of various bioactive molecules. On the basis of many pre-clinical researches, clinical studies have been conducted regarding their use in plastic and aesthetic surgery. However, their applications, efficiency and security are still controversial. In this paper, clinical studies of their use in fat grafting, facial rejuvenation and the treatment of scar and alopecia were reviewed. Related Articles | Metrics