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Preparation and application of collagen-based biomaterials in bone and cartilage regeneration and repair DUAN Jing, YANG Bing Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 707-.   Abstract （328）      PDF（pc） （1040KB）（748）       Knowledge map    Save Collagen-based biomaterials can be used as ideal biomaterials for bone and cartilage regeneration and repair due to their good biological activity and biocompatibility. However， due to the weak macroscopic mechanical properties of collagen， which cannot meet the mechanical requirements of natural cartilage and bone tissue， the mechanical properties are often improved by cross-linking modification. In addition， different material forms require different forming technologies. In this paper， the cross-linking methods and forming technologies of collagen-based biomaterials were reviewed， and the construction of collagen scaffolds and the selection of implanted cells for bone and cartilage regeneration and repair in recent years were summarized， aiming to provide clues and ideas for the development of collagen-based biomaterials preparation technology and the transformation of clinical applications. Related Articles | Metrics | Comments（0）

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The application of artificial intelligence（AI）in rhinoplasty PAN Xingyi, LI Zijun, ZHEN Yonghuan, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 659-.   Abstract （317）      PDF（pc） （1055KB）（639）       Knowledge map    Save With the advancement of medical technology and the improvement of patients' demand for personalized aesthetics， rhinoplasty has become one of the most challenging surgeries in plastic surgery. It requires not only precise anatomical knowledge but also a surgical plan that incorporates the patient's unique facial features and aesthetic expectations. In recent years， the application of artificial intelligence （AI） in the medical field has been booming， with its data analysis capabilities and automation offering new solutions for rhinoplasty. This review aimed to explore the specific applications of AI in rhinoplasty， including how AI can be used for preoperative planning， surgical navigation， and postoperative evaluation. By analyzing the key roles of AI in rhinoplasty， this paper highlighted the importance of AI in enhancing surgical precision， personalized design， and outcome predictability. Additionally， it discussed the potential value of AI in optimizing surgical plans， reducing complication risks， and improving patient satisfaction. Moreover， this paper addressed the limitations of AI in rhinoplasty， particularly its applicability in clinical decision-making and the challenge of insufficient personalization in aesthetic design， and prospected the future improvement direction of the AI technology and its further potential in rhinoplasty. Related Articles | Metrics | Comments（0）

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Preliminary clinical efficacy observation of lymphatic/lymph node-venous shunt surgery in cervical region Ⅱ/Ⅲ for the treatment of Alzheimer’s disease· LI Ke, WEN Ke, AI Songtao, et al Journal of Tissue Engineering and Reconstructive Surgery    2025, 21 (1): 10-.   Abstract （298）      PDF（pc） （807KB）（73）       Knowledge map    Save Preliminary clinical efficacy observation of lymphatic/lymph node-venous shunt surgery in cervical region Ⅱ/Ⅲ for the treatment of Alzheimer’s disease LI Ke1 ， WEN Ke1 ， AI Songtao2 ， YAN Jia3 ， WANG Haiya4 ， LI Zan5 ， LI Bin6 ， MIN Peiru1 ， MENG Xinxian1 ， WANG Xueqian1 ， HAN Chengyao1 ， SUN Yuhao1 ， LIU Jiaqi7 ， WU Jingshu1 ， WEI Yanxue1 ， SONG Xingqi1 ， ZHANG Yixin1 . 1 Department of Plastic and Reconstructive Surgery， Shanghai Ninth People’s Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200011， China；2 Department of Radiology， Shanghai Ninth People’s Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200011， China；3 Department of Anesthesiology， Shanghai Ninth People’s Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200011， China；4 Department of Geriatrics， Shanghai Ninth People’s Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200011， China；5 Department of Oncoplastic Surgery， Hunan Cancer Hospital， Changsha 410008， China；6 Department of OtorhinolaryngologyHead and Neck Surgery， Sichuan Provincial People's Hospital， Sichuan Academy of Medical Sciences， Chengdu 610041， China；7 Department of Burn and Plastic Surgery， Shanghai Ruijin Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200025， China. Corresponding author： ZHANG Yixin （E-mail： zhangyixin6688@163.com）. therapeutic principles and efficacy of this method in treating Alzheimer’s disease （AD）. Methods Patients underwent the lymphatic/lymph node-venous shunt surgery in the cervical Ⅱ/Ⅲ region. Cognitive assessments， including the MMSE，MoCA， and NPI， were conducted before and one month after surgery to evaluate symptom improvement. PET-CT was used to quantitatively analyze changes in intracranial Aβ protein levels. Color Doppler ultrasound was employed to measure the patency of the internal jugular vein and detect wall-adherent thrombus formation. Postoperative follow-up within one month included monitoring wound healing， hematoma or fluid accumulation， lymphatic leakage， postoperative delirium，headaches， and other discomforts. Results This study included 7 AD patients （3 males and 4 females） with an average age of 65（57-71） years. All patients had AD with dementia. Compared to preoperative scores， one month postoperatively， MMSE improved by 0（-2，1） points （P=0.581）， MoCA improved by 0（-1，1） points （P=1.000）， NPI （frequency× severity） improved by 9（9，20） points （P=0.043）， and NPI caregiver distress improved by 2（2，6） points （P=0.026）. Color Doppler ultrasound indicated patent internal jugular venous flow without wall-adherent thrombus. No adverse reactions were observed. Three patients underwent PET-CT one month postoperatively， showing an average reduction of 12.3% in whole-brain Aβ protein levels. Conclusion The proposed lymphatic/lymph node-venous shunt surgery in the cervical Ⅱ/Ⅲ region can divert the extracranial segment of intracranial lymphatic drainage， promote the clearance of pathological proteins， reduce intracranial Aβ protein levels， and improve clinical symptoms. This procedure may become a viable treatment option for AD， advancing the development of surgical interventions for AD. Related Articles | Metrics | Comments（0）

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Expression of TLR4/NF-κB signaling pathway in scar cancer tissues and effect of TAK-242 on scar cancer cells HUO Wenliang, TIAN Xiaorui, ZHANG Jinruo, et al Journal of Tissue Engineering and Reconstructive Surgery    2025, 21 (1): 14-.   Abstract （286）      PDF（pc） （7568KB）（313）       Knowledge map    Save Objective To analyze the expression of Toll like receptor 4（TLR4） and nuclear factor kappa B （NF-κB） in scar cancer tissues， and to investigate the effect of TLR4 inhibitor TAK-242 on scar cancer cells. Methods 20 cases of normal skin tissue， 20 cases of hypertrophic scar tissue， and 20 cases of scar cancer tissue were collected. Immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR） were used to detect the expression of TLR4 and NF-κB protein and mRNA. The relationship between the expression of TLR4， NF-κB and clinical pathological factors such as patient gender， age， and tumor differentiation degree was analyzed. Scar cancer cells were cultured in vitro and treated with TLR4 inhibitor TAK-242. Cell proliferation activity was detected by MTT assay， and the expression of TLR4， NF- κB， MMP9， and TGF-β1 proteins was detected by Western blot assay. Results Compared with the control group， the expression of TLR4， NF-κB protein and mRNA in scar tissue and scar cancer tissue was significantly increased（P< 0.05）； Compared with scar tissue， the expression of TLR4， NF-κB protein and mRNA in scar cancer tissue was significantly increased （P<0.05）. The expression of TLR4 and NF- κB in scar cancer tissue was related to the degree of tumor differentiation （P<0.05）. After intervention with TLR4 inhibitor TAK-242， the proliferation activity of scar cancer cells was  significantly reduced （P<0.05）， and the protein expression of TLR4， NF- κB， MMP9， and TGF-β1 was downregulated （P< 0.05）. Conclusion The TLR4/NF-κB signaling pathway is involved in the pathological process of scar tissue carcinogenesis， and the TLR4 inhibitor TAK-242 can inhibit scar cancer proliferation， which is expected to become a new target for scar tissue treatment. Related Articles | Metrics | Comments（0）

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Application of cerium oxide nanoparticles in wound healing ZHU Yulin, LIU Jing, DENG Guoying Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 574-.   Abstract （268）      PDF（pc） （948KB）（893）       Knowledge map    Save Wound healing is a complex physiological process， which can be divided into four main stages： Hemostasis， inflammation， proliferation and migration， and tissue remodeling. At the same time， granulation formation， inflammatory cell infiltration， necrotic tissue cleaning， scar remodeling and other reactions are involved. In some pathological cases， the natural healing process of the wound may be stalled， resulting in repeated attacks and even death from shock. In recent years， the search for high safety and high efficiency medical materials has attracted much attention from researchers. Among them， cerium oxide nanoparticles are a unique material which can promote wound healing and play an important role in all stages of wound healing. Nano-cerium oxide particles not only have antioxidant， antibacterial and anti-inflammatory functions， but also can be used as drug carriers in wound repair to ensure the benign absorption of the body， and can be used as tissue scaffolds to promote the development and maturity of new structures. Therefore， exploring the role of cerium oxide nanoparticles in wound healing may be a new strategy for wound repair. In this paper， the effects of cerium oxide nanoparticles on wound repair were reviewed to provide references for their further application and development in the future. Related Articles | Metrics | Comments（0）

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Clinical application of artificial dermis in the surgery of recurrent finger/toe mucocele ·ZHAO Weichao, WEI Bin, LIU Jinyang, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 682-.   Abstract （263）      PDF（pc） （10937KB）（22）       Knowledge map    Save Objective To explore the clinical effect of artificial dermis Pelnac in repairing soft tissue defects after resection of recurrent digital/toe mucocele. Methods A total of 32 patients with recurrent digital/toe mucocele who were admitted from May 2015 to January 2024 were retrospectively analyzed. Cyst and osteophyte excision and the artificial dermis Pelnac repair were performed. Results All 32 patients were followed up for 3 to 36 months （average 21 months）. The artificial dermis was removed 4 weeks after surgery， and the wound was completely epithelialized 6 to 8 weeks after surgery. The wound healed well without infection or cyst recurrence. After surgery， the sensation and range of motion were scored according to the trial standard of the upper limb function assessment of the Chinese Medical Association of Hand Surgery. The sensory scores were S4 in 28 cases and S3+ in 4 cases. The range of motion score was excellent in 30 cases and good in 2 cases. The average Vancouver scar scale （VSS） score was 1.23. The visual analogue scale （VAS） score was 0. Conclusion The repair of soft tissue defect after finger/toe mucocele resection with artificial dermis Pelnac is simple in repair technique， short in operation time， without collateral damage， significantly reducing cyst recurrence， and has good clinical effect. Related Articles | Metrics | Comments（0）

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Preliminary research of Da Vinci robot-assisted nipple sparing mastectomy and immediate breast reconstruction with gel implant and the latissimus dorsi muscle flap Chen Kuo, LV Pengwei Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 522-.   Abstract （245）      PDF（pc） （14143KB）（14）       Knowledge map    Save Objective To explore the effectiveness and practicability of robot-assisted nipple sparing mastectomy and immediate breast reconstruction with gel implant and the latissimus dorsi muscle flap （LDMF）. Methods A retrospective analysis of 14 robot-assisted breast reconstruction with LDMF completed from September 2022 to September 2023 in a single medical center was conducted. The cohort was divided into two groups： those with skin islands （n=5） and those without （n= 9）. Variables such as surgical technique， operative duration and perioperative complications were scrutinized. Results The study encompassed 14 patients. Among these，8 underwent contralateral breast reconstruction， and one opted out of using a gel implant. Average total operative times were （392.1±61.2） minutes for the group with island flap and （351.6±67.4） minutes for the group without island flap. And indwelling drains and length of hospital stay were （11.2±0.7） d and （18.4±1.5）d for the group with island flap，（9.0±1.1） d and （11.3±1.1） d for the group without island flap. The findings were statistically insignificant due to the limited sample size and the bias between the two groups caused by the inclusion of contralateral surgery and axillary surgery. There were no postoperative necrosis and perioperative complications of the nipple areolar complex. No recurrence or death occurred during a mean follow-up of （3±1） months. All patients were satisfied with the aesthetic results after operation. Conclusion Robot-assisted nipple sparing mastectomy and immediate breast reconstruction with gel implant and LDMF is a safe surgical procedure. LDMF without island flap procedure is more advantageous in terms of drain retention time and hospitalization time， and has good cosmetic results. However， the technique  remains challenging and requires a long learning curve for the surgeon Related Articles | Metrics | Comments（0）

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Generating insulin-producing cells from human adipose-derived stem cells：Comparison of three differentiation protocols YANG Jing, LIU Xingran, YIN Xiya, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 507-.   Abstract （239）      PDF（pc） （6161KB）（418）       Knowledge map    Save Objective The induced differentiation of adipose stem cells into insulin-secreting cells is a new strategy for the treatment of diabetes mellitus. The efficiency of the induced differentiation schemes reported so far varies greatly. This study aims to compare the differentiation efficiency of three induced differentiation schemes. Methods Fat samples from the same batch were collected to extract fat stem cells for passage culture， and the third-generation fat stem cells were self-aggregated into balls for induction and differentiation in different phases （one-step method， two-step method and three-step method）. The expression levels of key genes and proteins in the products of the three schemes at the corresponding stages were detected by PCR and immunofluorescence， and the function of insulin secreting cells was detected by glucose stimulation of insulin secretion experiment. Results Compared with other schemes， the gene expressions of the key transcription factors INS，NKX6.1 and PDX1 in the insulin secreting cells induced by the three-step method were significantly increased， and there were significant differences. Key protein C-peptide and NKX6.1/PDX1 were co-expressed in insulin-secreting cells. Functionally， the insulin secreting cells induced by the three-step method can significantly increase insulin secretion under high glucose stimulation. Conclusion Under the same differentiation conditions， the differentiation efficiency of adipose stem cells induced into insulin secreting cells by the three-step method is superior to that by the two-step method and the one-step method， which provides a basis for establishing an efficient and safe induction differentiation scheme of insulin secreting cells. Related Articles | Metrics | Comments（0）

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Research progress on modified hydroxyapatite-based bone repair materials HUANG Leiyun, BAI Zhenglin, FU Guangjie, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 568-.   Abstract （237）      PDF（pc） （1027KB）（178）       Knowledge map    Save As a kind of biomedical material， modified hydroxyapatite-based material has good mechanical properties， biocompatibility and osteoinductive ability. It has the advantages of similar composition with natural bone， not easy to cause immune rejection， good biological activity and compatibility， non-toxic， degradable， easy to prepare and process， and makes up for the shortcomings of natural bone material transplantation. In this paper， the biological modification， physicochemical modification and composite modification methods of the material were introduced， and the effects of different modification methods on the properties of the material were compared. In addition， the current problems and challenges of modified hydroxyapatite-based material were presented in the discussion section， and the future development of this kind of material was also prospected. Related Articles | Metrics | Comments（0）

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Research progress of 3D adipose tissue model ZHU Congxiao, YIN Ningbei Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 562-.   Abstract （236）      PDF（pc） （975KB）（1181）       Knowledge map    Save As the largest endocrine organ and the main energy storage system in the human body， adipose tissue plays a pivotal role in regulating energy homeostasis. Pathological conditions such as insulin resistance， chronic inflammation and mitochondrial dysfunction are closely related to adipose metabolic disorders. In vitro models are significant tools for studying the mechanism of associated disorders. At present， three-dimensional culture is a common model for in vitro adipose tissue research because it can imitate the process of adipose metabolism to a certain extent. In order to serve as a resource for future scientific investigations， an overview of three-dimensional adipose tissue models and their properties was provided in this paper. Related Articles | Metrics | Comments（0）

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“Starship”in skin regeneration：Exploring the breakthroughs and potential of acellular dermal matrix applied in skin expansion LUO Xusong Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 503-.   Abstract （225）      PDF（pc） （950KB）（681）       Knowledge map    Save The simultaneous reconstruction of appearance and function following large-area skin defects remains an unresolved challenge in clinical practice. Although skin expansion technology has been widely employed in tissue repair and reconstruction， its development has stagnated， primarily due to low expansion efficiency， high complication rates， and poorly understood mechanisms. To address this issue， an innovative approach utilizing acellular dermal matrix was proposed to assist in skin expansion and achieved promising preliminary clinical results. Acellular dermal matrix provides a “biocompatible” interface， balances mechanical forces， and serves as an active scaffold to support various cell types. However， its specific mechanism of action during the skin expansion process warrants further investigation. By acting as an expansion companion， acellular dermal matrix synergistically enhances skin expansion and holds the potential to break through current expansion limitations， thereby unlocking the full potential of skin expansion technology. Related Articles | Metrics | Comments（0）

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Research on hair transplantation and hairline correction to reduce the facial apparent length of long-faced females SHEN Lihong, GUO Yaqi, JIANG Wenbin, et al Journal of Tissue Engineering and Reconstructive Surgery    2025, 21 (1): 55-.   Abstract （213）      PDF（pc） （8156KB）（7）       Knowledge map    Save Objective To introduce an innovative technique for improving the appearance of elongated faces by modifying the hairline via hair transplantation， thereby enhancing facial proportions across frontal， oblique， and lateral perspectives. Methods Fifty-three female patients were included in this study. Facial measurements included the midline length and the lengths of the upper， middle， and lower thirds of the face. Additionally， four distances were assessed in oblique and lateral views： The vertical distances from the temporal hairline to the menton and from the sideburn to the menton. The hairline was corrected with hair transplantation， and the results were assessed by the patient and surgeon using the Likert scale. Results Post-transplantation， the central hairline was advanced， shortening the upper third of the face and decreasing the vertical distances from the temporal hairline and sideburn to the menton. Both patients and surgeons evaluated the outcomes using the Likert scale， with patients reporting an average score of 4.5. No adverse events were noted. Two patients required additional procedures to enhance hair density in the transplant region. Conclusion Hairline modification through hair transplantation presents an effective strategy for achieving facial balance and symmetry， offering a promising solution for women seeking to correct an elongated facial appearance. Related Articles | Metrics | Comments（0）

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Clinical application of autologous cell micrograft extracts for the treatment of androgenetic alopecia in 20 cases TAN Pohching, LENG Bing, LIU Qing Journal of Tissue Engineering and Reconstructive Surgery    2025, 21 (1): 50-.   Abstract （205）      PDF（pc） （10811KB）（27）       Knowledge map    Save Objective To explore the efficacy and safety of autologous cell micrograft （ACM） extracts in the treatment of androgenetic alopecia （AGA）. Methods The situation of AGA patients receiving ACM extract injection treatment in alopecia region was retrospectively analyzed. The cell count， survival rate and cell subtypes of ACM extract suspensions were analyzed by flow cytometry. The patients were followed up for （12±2） weeks after treatment. The effectiveness of the treatment was comprehensively evaluated by the photos before and after treatment， dermoscopy images and patient satisfaction questionnaire. At the same time， the injection area was observed for infection， swelling， rupture and other adverse reactions to evaluate the safety of treatment. Results The study included 20 patients with AGA. The suspension of ACM extract contained up to 106 highly viable cells， with DPs comprising the majority. Treatment with ACM extract resulted in significant improvement in hair loss appearance and a notable increase in hair coverage （P<0.05）. Dermoscopic examination revealed a significant increase in overall hair density and a higher terminal-to-vellus hair ratio （T/V ratio）. Patient satisfaction was high， with reports of reduced scalp oiliness and itching post-treatment. Additionally， ACM extract treatment can improve depigmentation. None of the 20 patients experienced any treatment-related adverse reactions during follow-up. Conclusion　The application of ACM extract for the AGA treatment is safe and effective. This treatment method may serve as a novel approach to the AGA treatment as the injection of ACM extract was technologically simple and easy to use. Related Articles | Metrics | Comments（0）

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The clinical efficacy of blepharoplasty combined SVF-gel grafting for upper eyelid rejuvenation in Asian mid-aged and elderly female NIE Lili1, LU Hua1, GUO Meili, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 542-.   Abstract （199）      PDF（pc） （9192KB）（33）       Knowledge map    Save Objective To explore the feasibility of SVF-gel fat grafting to sunken upper eyelid combined with blepharoplasty to improve upper eyelid laxity and depression in middle-aged and elderly women. Methods In this study， 214 patients who underwent blepharoplasty and ROOF layer SVF-gel fat filling were retrospectively studied. All patients underwent abdominal liposuction and blepharoplasty under local anesthesia， and the SVF-gel was injected into the layers between orbicularis oris muscle and periosteum layer of designed grafting area in the form of micro-droplets in ROOF layers by using 25g blunt needles， finished by overcorrection of sunken upper eyelid. The laxity of upper eyelid was then corrected by blepharoplasty with bridge skill， fully released septum fat and removed the appropriate amount of skin as well as muscle. Standard photos were taken before and on the day after surgery，6 months after surgery， and aesthetic evaluation of upper eyelid depression， satisfaction score and postoperative effect evaluation were also performed. Postoperative effect evaluation included improvement of depression， double eyelid symmetry and complications. Results The 214 patients were all female， with an average age of （38.61±10.22） years. The overall follow-up period was 6 to 23 months， and the average follow-up period was （12.36±3.132） months. All the patients underwent bilateral blepharoplasty with SVF-gel fat grafting to ROOF  layer， the amount of fat grafting ranged from 1.2-2 mL on one side， mean （1.535±0.282） mL on one side. The final overall ratio of upper eyelid depression scored 3.569±1.517， while the preoperative ratio scored 1.115±0.502（n=428，P<0.000 1）， indicating a significant improvement in upper eyelid depression. The GAIS score was 2.384±0.193 （n=214）. Only 14 （6.54%） patients presented with hematomas within 7 days of surgery which resolved completely within two weeks. Eventually at six months after surgery，47（21.96%） patients still had insufficient improvement of the upper eyelid depression and required secondary fat grafting to achieve a better result， with a reoperation rate of 21.96%. Majority of patients were satisfied with the results of the procedure， with an average satisfaction score of （3.974±0.473） out of 5（full mark）. Conclusion SVF-gel fat grafting to ROOF layer combined with blepharoplasty is safe and effective in improving upper eyelid laxity and depression in middle-aged and elderly women. SVF-gel has a good effect in the fine filling of the upper eyelid， and it can be further developed with other surgical methods in the future. Related Articles | Metrics | Comments（0）

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Advances in the application of accurate soft tissue measurement in facial aesthetics researc YAN Sijing, YU Nanze, LONG Xiao Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 648-.   Abstract （198）      PDF（pc） （1077KB）（63）       Knowledge map    Save With the rapid advancement of medical aesthetics， precise soft tissue measurement technologies have provided a new possibility for quantitative analysis of facial aesthetics. This review outlined common facial soft tissue measurement methods， highlighting their advantages and limitations. Additionally， progress in automated measurement technologies， particularly deep learning-based landmark extraction and measurement techniques， has emerged as a reliable and accurate approach to precision soft tissue analysis. This paper provided a comprehensive technical reference for facial aesthetics research and discussed the potential for wider application of automated measurement technologies in this domain. Related Articles | Metrics | Comments（0）

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Protective effects of Thanaka extract against skin photoaging damage of HaCaT cells caused by UVB XU Wanyu, ZHANG Yixin Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 516-.   Abstract （193）      PDF（pc） （3163KB）（108）       Knowledge map    Save Objective To investigate the protective effect of Thanaka extract against UVB-induced photoaging damage in skin. Methods HaCaT cells were treated with 0-25 μg/mL Thanaka extract and 30 mJ/cm2UVB. CCK-8 method was used to observe the effect of Thanaka extract and its effect on the viability of HaCaT cells after UVB irradiation. HaCaT cells were treated with low （3 μg/mL）， medium （6 μg/mL） and high （12 μg/mL） concentrations of Thanaka extract and 30 mJ/cm2 UVB. DCFH-DA fluorescent probe was used to detect intracellular reactive oxygen species （ROS） content； JC-1 fluorescent probe was used to detect mitochondrial membrane potential； Superoxide dismutase （SOD） and malondialdehyde （MDA） were used to detect the levels of antioxidant and oxidative damage. Results The treatment of 0-25 μg/mL of Thanaka extract at all concentrations had no significant effect on cell viability. The viability of HaCaT cells was increased after treatment with 12 μg/mL of Thanaka extract compared with that of the UVB group （P<0.05）. Further experiments showed that ROS content decreased （P<0.05）， SOD activity increased （P<0.05） in the medium and high concentration of Thanaka extract groups；MDA level decreased （P<0.05） and mitochondrial membrane potential rebounded in the low， medium， and high concentration of Thanaka extract groups compared with the UVB group. Conclusion Thanaka extract can promote the viability and antioxidant level of HaCaT cells after UVB irradiation， and reduce the oxidative stress damage produced by UVB， and its underlying mechanism may be related to mitochondrial function Related Articles | Metrics | Comments（0）

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Analysis of continuing education for fellowship in plastic and reconstructive surgery at a tertiary hospital in Shanghai ZHOU Renpeng, YU Yan, WANG Danru Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (5): 595-.   Abstract （191）      PDF（pc） （920KB）（115）       Knowledge map    Save Objective　 To analyze the current situation and problems of continuing education for plastic surgery fellowships. Methods　The data and information of plastic surgery fellowships from a tertiary hospital in Shanghai from 2019 to 2023 were collected and analyzed. Results　In the past five years， a total of 546 fellowships have been trained in a tertiary hospital in Shanghai. The majority of training doctors were male， aged 31-40 years old， and most of them were the backbone of local business with intermediate titles. Bachelor's degree and master's degree were the main qualifications of fellowships， and most of them came from tertiary hospitals. Among them， less than half were major in plastic surgery， and the characteristic of interdisciplinary continuing education was obvious. Conclusion　 Plastic surgery covers a wide range of fields， and the teaching and training of fellowships should pay attention to systematization and specialization to achieve highquality continuing education goals. Related Articles | Metrics | Comments（0）

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#br# Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 702-.   Abstract （186）      PDF（pc） （1165KB）（1067）       Knowledge map    Save Distal arthrogryposis represents a group of congenital malformations with genetic factors， and distal arthrogryposis type 9 is attributed to mutations in the FBN2 gene. Differing from other subtypes where muscular tissues are affected，FBN2 gene mutation primarily impacts the embryonic development of connective tissues， giving rise to distinctive clinical phenotypes. To date， the specific pathogenic mechanisms of this condition remain elusive. In this paper， the recent research progress of distal arthrogryposis type 9 was reviewed， aiming to enhance our understanding of the disease. With the advancement and widespread use of sequencing technologies， the potential discovery of additional mutation loci and patient cohorts holds promise for elucidating genotype-phenotype correlations. Furthermore， there is a pressing need to promote the development of appropriate cellular and animal models to further elucidate its pathogenic mechanisms， offering novel avenues for disease diagnosis and therapeutic interventions. Related Articles | Metrics | Comments（0）

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Calendula moisturizing repair spray and facial mask after 1 064 nm dot matrix laser car LU Wenting, LU Nan, DONG Jiying Journal of Tissue Engineering and Reconstructive Surgery    2025, 21 (1): 65-.   Abstract （186）      PDF（pc） （994KB）（34）       Knowledge map    Save bjective To evaluate the efficacy and safety of calendula moisturizing repair spray and facial mask for postoperative care after 1 064 nm dot matrix laser. Methods Thirty-six patients with 1 064 nm facial dot matrix laser were divided into experimental group and control group. The control group received conventional care， and the experimental group received laser postoperative care with calendula moisturizing repair spray and facial mask based on the control group. The trans-epidermal water loss， skin water content， erythema index， light intensity and the occurrence of adverse reactions were analyzed and compared between the two groups. Results There was no significant difference in trans-epidermal water loss between experimental group and control group at each time point after treatment （P>0.05）. Immediately after treatment，10 days after treatment and 28 days after treatment， the skin water content of experimental groups was higher than that of control group （P<0.05）， and the erythema index of experimental groups was lower than that of control group （P<0.05）. There were no obvious adverse reactions in the face of patients of the two groups during the treatment. Conclusion Calendula moisturizing repair spray and facial mask applied to wounds after 1 064 nm fractional laser surgery are safe and effective auxiliary means with good nursing effect. Related Articles | Metrics | Comments（0）

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Keystone flap and latissimus dorsi flap in repairing popliteal fossa defect：A case report Aierbanjiang·Shali, Xiriaili·Kadier, CHENG Erlin, et al Journal of Tissue Engineering and Reconstructive Surgery    2024, 20 (6): 698-.   Abstract （185）      PDF（pc） （8044KB）（64）       Knowledge map    Save Related Articles | Metrics | Comments（0）