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    01 August 2022, Volume 18 Issue 4 Previous Issue    Next Issue
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    Novel man-machine cooperative robot-assisted craniotomy: A pilot animal experiment on feasibility and safety 
    LI Xingzhou, ZHANG Ziwei, YAN Yingjie, CHEN Xiaojun, ZHANG Yan
    2022, 18 (4):  289. 
    Abstract ( 402 )   PDF (900KB) ( 223 )  
    Objective To introduce a novel man-machine cooperative robot, and to carry out animal experimental study on its feasibility of assisting craniotomy. Methods Four beagles underwent craniotomy. Titanium nails were implanted into the skull before preoperative CT scans. During the craniotomy, the head was immobilized and cone beam computed tomography (CBCT) was adopted for intraoperative scans. The robot and head were registered by an end-effector with labeled steel balls. The pre-operative design was aligned to the titanium nails in intra-operative CT scans. Safety was assessed by rate of dural tears and postoperative complications. Accuracy was evaluated by coincidence rate of the cranial window and deviation between preoperative design and postoperative CT scans. Results All operations were successful. No dural tears or postoperative complications were observed. The mean coincidence rate of the cranial window was 94.08%±2.32%. The absolute error of the center point of the drilling holes was (0.9394±0.1497) mm, with an angle error of 3.37° ±1.47° . The mean linear deviation value was (1.056±0.129) mm in the upper surface, and (1.087±0.096) mm in the lower surface. Conclusion Thisnovel man-machine cooperative robot is feasible and safe for assisting craniotomy. More technique should be integrated in its next generation for more convenient use.
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    Effect of mesenchymal stem cells-derived exosomes on hyperglycemic injury of human peritoneal mesothelial cells
    JI Ouyang, FANG Junyan, SONG Ahui, TONG Yan, WEI Shan, CHEN Zhihao, LIU Yingli
    2022, 18 (4):  294. 
    Abstract ( 183 )   PDF (1275KB) ( 197 )  
    Objective To explore the effect of  mesenchymal stem cells derived exosomes (MSCs-Exo) on peritoneal mesothelial injury induced by high glucose. Methods MET-5A cells were divided into three groups: normal control group (no treatment), high glucose group (treated with high glucose) and co-culture group (treated with high glucose and MSCs-Exo). High glucose concentration and MSCs-Exo concentration with the best stimulation effect were selected by CCK-8 method. After 24 hours of treatment in each group, the cells and supernatant were collected. The cells proliferation activity was determined by CCK-8, cell apoptosis was tested by flow cytometry, the expression of cytokines IL-6 and TNF-α was detected by qRT-PCR, and the level of IL-6 in culture supernatant was detected by ELISA. Results The cell viability of high glucose group was significantly lower than that of normal control group (P<0.001), and the apoptosis rate, expression of IL-6 and TNF-α were significantly higher (P<0.001). Compared with the high glucose group, the cell viability of the co-culture group was increased (P<0.001), and the apoptosis rate, IL-6 and TNF-α expression were significantly down-regulated (P<0.05). Conclusion High glucose has significant pro-apoptotic and pro-inflammatory effects on mesothelial cells. While  MSCs-Exo can promote mesothelial cells proliferation and inhibit the occurrence of apoptosis and the expression of inflammatory factors caused by high glucose.
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    Construction of enhanced green fluorescent protein labeling bladder cancer exosomes and its tracing application in vitro
    CHEN Hui, HOU Aihua, WANG Peipei, MEI Xiaofeng.
    2022, 18 (4):  300. 
    Abstract ( 329 )   PDF (1686KB) ( 242 )  
    Objective To obtain EGFP labeling exosomes of bladder cancer cells for tracking exosomes in vitro by constructing a bladder cancer cell line stably expressing CD63-EGFP fusion protein. Methods The CD63 gene was inserted into pLVX-EGFP-puro plasmid by molecular cloning to construct the expression vector of CD63-EGFP fusion protein. Bladder cancer cell lines stably expressing CD63-EGFP fusion protein were obtained by lentivirus transfection. Exosomes were extracted by ultracentrifugation and identified by transmission electron microscopy, particle size analysis and Western blot. The uptake of bladder cancer cell exosomes by SV-HUC-1 cells and peripheral blood mononuclear cell (PBMC) was detected through co-culture. Results A Bladder cancer cell line stably expressing the CD63-EGFP fusion protein was constructed, and the secreted exosomes were consistent with the classic characteristics of exosomes. SV-HUC-1 cells and Monocytes in PBMC can uptake bladder cancer cell-derived exosomes in vitro. Conclusion Bladder cancer cells expressing CD63-EGFP fusion protein can secrete fluorescent exosomes, which can be used for tracing of exosomes in vitro.
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    Effect of ubiquinone on ischemia-reperfusion injury of rat dorsal skin flap
    ZHANG Xiaoyi, ZHANG Hao.
    2022, 18 (4):  305. 
    Abstract ( 148 )   PDF (1437KB) ( 112 )  
    Objective To explore the role of ubiquinone in ischemic reperfusion injury  by rat dorsal flap model. Methods A total of 120 healthy adult male SD rats were randomly divided into three groups: sham surgery group, experimental group and control group, with 40 rats in each group. A flap of 9.0 cm×6.0 cm was designed on the back of the rat and five of the six blood supply vessels were ligated, and the experimental group and the control group were molded. The experimental group received 50 mg/kg ubiquinone; The sham surgery group and the control group were given the same amount of cooking oil every day, and the gastric volume was 5 mL/kg per day. On the 1st, 3rd, 5th and 7th days after the model was established, 10 rats were randomly sacrificed in each group to observe the corresponding indicators: the flap survival rate, flap tissue HE staining, flap tissue SOD activity and MDA content. Results The flap survival rate, flap tissue HE staining, flap tissue SOD activity and MDA content of flap tissue were statistically analyzed. The sham operation group was superior to the experimental group and the experimental group was superior to the control group at all time points (P<0.05) except for the MDA content of skin flap between the sham operation group and the experimental group on the 7th day (P>0.05). Conclusion Ubiquinone can effectively alleviate ischemia-reperfusion injury of oversized flaps on the back of rats.
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    Keystone perforator flap in repairing soft tissue defect of hand and foot 
    CAI Feiyu, CHENG Erlin, Aihemaitijiang·Yusufu.
    2022, 18 (4):  311. 
    Abstract ( 293 )   PDF (687KB) ( 161 )  
    Objective To explore the application and clinical effect of keystone perforator flap in repairing soft-tissue defect of hand and foot. Methods From February 2015 to December 2020, a total of 24 patients (14 males and 10 females) with soft tissue defects in the hand and foot were repaired by the keystone perforator flaps. Of these, 18 had hand defects (13 palmar and 5 dorsal) and 6 had foot defects (3 plantar and 3 dorsal). The defects were located at the joint site in 7 cases. The defect area ranged from 1.0 cm×1.5 cm to 3.0 cm×5.5 cm, and the flap harvesting area ranged from 1.0 cm×2.0 cm to 4.5 cm×8.0 cm. Selective fixation was performed for 2 to 3 weeks according to the defect site and area after surgery. Results All patients were followed up for 13 months on average (6-24 months). All incisions healed by first intention. The surface of the flap was smooth and there was no color difference with the surrounding skin. The flap showed sensitivity to touch, good functional recovery, and high patient satisfaction. Conclusion Keystone perforator flap is a reliable method for repairing soft tissue defects in the hand and foot, which not only has few complications but also can meet the appearance and functional needs.
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    Effect contrast of different types of skin flaps in repairing soft tissue defects of the foot and ankle 
    SHI Jianguo, WANG Jihong, YANG Yanjun.
    2022, 18 (4):  315. 
    Abstract ( 150 )   PDF (905KB) ( 144 )  
    Objective The effects of sural nerve nutritional vascular flap, medial foot flap, lateral heel flap and foot and ankle epithelial flap for skin flap repair in patients with soft tissue defects of the foot and ankle were compared. To explore the effect of different types of skin flaps in repairing soft tissue defects of the foot and ankle. Methods The data of 80 patients with foot and ankle soft tissue defects was retrospectively analyzed. Among the 80 patients, 25 patients were repaired with sural nerve nutritional vascular flap (group A), 20 patients with medial foot flap (group B), 15 patients with lateral flap (group C), and 20 patients with ankle and ankle flap (group D). All the patients were followed up for 6 months after operation. The post-healing situation and the American Ankle Orthopedics Association's Ankle Scale (AOFAS) were compared among all the groups. Results There were no significant differences in the number of cases of infection, vascular crisis, good appearance, soft texture and recovery of walking function among all groups (P>0.05), the flap survival rate in group A were higher than that of group C and group D (P<0.05). The excellent and good rate of AOFAS ankle hindfoot score of group A was higher than that of the other three groups (P<0.05). Conclusion The soft tissue defect of foot and ankle should be repaired according to the defect location, the sural nerve nutritional vascular flap is a good method for repairing the soft tissue defect of foot and ankle with high survival rate, which is beneficial to the functional recovery of the affected limb.
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    Experience in replantation of severed fingertip with long-term warm ischemia
    YANG Shenglin, LIU Chengwei, YUAN Honghao, PENG Long, GONG Hewei, GUAN Hanhui, CHEN Yu
    2022, 18 (4):  319. 
    Abstract ( 114 )   PDF (538KB) ( 202 )  
    Objective To explore the methods of replantation of severed fingertip with long-term warm ischemia. Methods From March 2017 to December 2019, 17 patients (19 fingers) with warm ischemia time of 8-19 hours were reviewed. Replantation was performed by emergency microsurgery. Results In this group, 16 fingers were successfully replanted and 3 fingers failed, with a success rate of 84.2%. Conclusion There is still a high possibility of successful replantation of severed fingertip with long-term warm ischemia, which should not be easily given up and lead to fingertip defect and partial functional impairment.
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    Clinical effect of bilayer artificial dermis combined with autologous skin graft for repairing finger skin soft tissue defect with tendon and bone exposure
    LI Ming, GUO Zhaodi, WANG Wei, CHEN Dongsheng, LI Tao.
    2022, 18 (4):  322. 
    Abstract ( 315 )   PDF (985KB) ( 144 )  
    Objective To explore the clinical effect of bilayer artificial dermis combined with autologous skin graft in repairing skin and soft tissue defects of fingers with tendon and bone exposure. Methods A total of 43 patients of finger skin and soft tissue defects with tendon and bone exposure admitted from September 2020 to October 2021 were retrospectively analyzed. Bilayer artificial dermis combined with autologous skin graft was used as the observation group (n=16) and pedicled flap was used as the control group (n=27). The treatment of the two groups were observed and compared, including the interval between two surgeries, the survival rate of skin graft/flap and the length of hospital stay. Vancouver scar scale (VSS) was used for scar score 3 months after operation. The recovery of finger sensation was scored before surgery, 1 month and 3 months after surgery, and two-point resolution test was performed 3 months after surgery. Results The interval between two surgeries and  the length of hospital stay in the observation group were both significantly longer thanthose in the control group (P<0.05), while there was no significant difference in the survival rate of skin graft/flap between the two groups (P>0.05). All patients recovered well 3 months after operation without obvious skin ulceration. There were no significant differences in the color, vascular distribution and softness of VSS scores between the two groups (P>0.05), and the thickness of observation group was significantly lower than that of control group (P<0.05). The sensory recovery score of observation group was significantly higher than that of control group at 1 and 3 months after operation (P<0.05). The sensory recovery scores of the two groups 1 month and 3 months after surgery were significantly higher than before surgery, and the sensory recovery scores of the two groups 3 months after surgery were significantly higher than 1 month after surgery (P<0.05). Three months after surgery, there was no significant difference in patients' two-point resolution of the two groups(P>0.05). Conclusion Bilayer artificial dermis combined with autologous skin graft can effectively repair the finger skin and soft tissue defects with tendon and bone exposure. Compared with pedicled skin flap, domestic bilayer artificial dermis Lando can achieve shorter hospital stay and more beautiful appearance of fingers, providing a new choice for clinical repair of such wounds.
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    Antibiotic bone cement combined with other comprehensive interventions in the treatment of diabetic foot osteomyelitis 
    BU Fanyu, GUO Xiaofeng, XU Peng, WANG Jin, XUE Mingyu, PAN Xiaoyun.
    2022, 18 (4):  328. 
    Abstract ( 203 )   PDF (1307KB) ( 121 )  
    Objective To evaluate the efficacy of vancomycin bone cement combined with other comprehensive interventions in the treatment of diabetic foot osteomyelitis (DFO). Methods A retrospective analysis of 93 patients with DFO (112 affected feet) who received vancomycin bone cement combined with other interventions was performed. Duration of oral and intravenous antibiotic use, length of hospital stay, ulcer healing time, recurrence rates, and amputation rates above the ankle were assessed at the last follow-up. Bacterial culture of secretion from deep wound surface of foot ulcer was conducted. Recovery of foot function was assessed using the Maryland standard. Results Eighty-seven patients with 105 involved feet were followed up successfully over an average period of 14 months. All wounds exhibited good union on follow up, and DFO was cured. The average durations of oral and intravenous antibiotic administrations were (12.2±1.5) and (10.8±2.5) days, respectively. The average duration of hospitalization was 14.0±2.7 days and the healing time of the ulcers was (37.8±6.3) days. Twenty-one patients (21 feet) were re-hospitalized after recurrence of foot ulcers, with a recurrence rate of 20% (21/105). No patients required amputation above the ankle. One hundred and forty-four pathogenic bacteria were co-cultured from the secretions of deep wounds from foot ulcers. According to the Maryland criteria, 31, 45, 26, and 3 feet were rated as excellent, good, fair, and failure, respectively. Overall, 72% were rated as excellent-good. Conclusion Antibiotic bone cement combined with other comprehensive interventions is effective and comprehensive in the treatment ofDFO, which is worthy of clinical application.
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    Application of ultrasound-assisted liposuction to shape female firm abdominals
    WANG Jian, WANG Jing, LI Hengsheng, WANG Bin, HUANG Kai, WEI Yuanqiang, , DING Xiaobang, CAI Chang.
    2022, 18 (4):  333. 
    Abstract ( 142 )   PDF (524KB) ( 137 )  
    Objective To explore the effect of ultrasonic-assisted liposuction in shaping female firm abdominals. Methods The abdominal liposuction area was marked before surgery, swelling fluid was injected, fat emulsification was assisted by ultrasound, and the lateral margin of the rectus abdominis muscle and the abdominal midline above the navel were strengthened to clearly reveal the anatomical shape of the rectus abdominis muscle. Results Ultrasound-assisted liposuction was applied in 28 patients. The rectus abdominis muscle was exposed satisfactorily after operation, and no complications such as burn and skin necrosis occurred. Conclusion Ultrasound-assisted liposuction can be applied to abdominal liposuction to reveal the three-dimensional shape of rectus abdominis muscle and achieve the effect of shaping firm abdominals.
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    Study of temporomandibular joint morphology after condylar fractures by CBCT and condylar volume analysis
    LI Yu, CHEN Linlin, HE Pinghua.
    2022, 18 (4):  336. 
    Abstract ( 128 )   PDF (561KB) ( 112 )  
    Objective To explore the value of condylar volume analysis based on cone beam CT(CBCT) in the measurement of the temporomandibular joint (TMJ) morphology of patients with condylar fractures. Methods From January 2017 to May 2019, 28 patients with condylar fracture were enrolled. CBCT images were analyzed. Preoperative and postoperative TMJ bone morphology indexes (left condyle volume and right condyle volume) were compared between the fractured side and the non-fractured side. Results The condylar volume on affected side after fracture operation was larger than that on non-affected side (P<0.05), and the condylar volume was reduced after surgery (P<0.05). Conclusion The changes of condylar volume of patients with condylar fractures before and after surgery have significant difference between the fractured side and the non-fractured side. This may be a new standard for postoperative TMJ functional evaluation.
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    Effect of parental accompanying on peri-anesthesia nursing care of children with congenital ptosis under the background of COVID-19 
    GAO Weiling, HU Yingqing.
    2022, 18 (4):  339. 
    Abstract ( 159 )   PDF (410KB) ( 81 )  
    Objective To explore the effect of parental accompanying on peri-anesthesia nursing care of children with congenital ptosis under the background of COVID-19. Methods From March 2020 to March 2021, 100 children with congenital ptosis were selected and randomly divided into two groups: Intervention group and control group, with 50 children in each group. The children in the intervention group were accompanied by their parents during anesthesia induction and postoperative recovery, while the children in the control group were not accompanied by their families. The vital signs and agitation scores were compared between the two groups during anesthesia induction and anesthesia recovery. Results Compared with the control group, the vital signs of children in the intervention group were more stable during peri-anaesthesia (P<0.05). Compared with the control group, the score of agitation in the intervention group was lower during peri-anaesthesia (P<0.05). Conclusion During the period of COVID-19, parental accompanying can effectively reduce the crying in operation room, reduce the risk of anesthesia and reduce the adverse reactions during anesthesia period, so as to ensure the safety of the whole anesthesia period and improve the quality of anesthesia nursing.
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    Clinical application of cold compress facial mask in pain management after laser therapy
    LU Wenting, CHEN Ping, BIAN Weiwei
    2022, 18 (4):  342. 
    Abstract ( 153 )   PDF (390KB) ( 219 )  
     Objective To explore the clinical effect of cold compress mask on pain care after laser treatment. Methods A total of 40 patients who underwent facial laser treatment from May 2018 to May 2019 were selected as the research subjects, and were divided into a control group and an experimental group strictly according to the method of random stratified sampling, with 20 cases in each group. The control group was given routine facial laser care after treatment, and the experimental group was given cold compress mask on the basis of the control group. The clinical efficacy, visual analog pain (VAS) score and VISIA test results were compared between the two groups. Results The clinical efficacy of the experimental group after nursing was better than that of the control group, and the difference was statistically significant (P<0.05). The VAS scores of the experimental group immediately after operation, 30 minutes after operation, and 4 hours after operation were lower than those of the control group, and the VISIA index of the experimental group after nursing was higher than that of the control group, and the difference was statistically significant (P<0.05). Conclusion Postoperative nursing of cold compress mask can effectively relieve the symptoms of pain, burning, redness and swelling after laser treatment, and improve patients' satisfaction with treatment.
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    Application of bioactive glasses on soft tissue repair 
    LI Wenbo, ZHANG Haoran, GUO Feng, YANG Songlin.
    2022, 18 (4):  345. 
    Abstract ( 362 )   PDF (509KB) ( 419 )  
    Bioactive glasses (BGs) have been widely used in regenerative medicine and tissue engineering. It can produce hydroxy carbonate apatite (HCA) when contact with body fluids, and thus closely bind with bone tissue, so it is often used in the replacement, regeneration and repair of bone defects or teeth. With the development of technology and bioactive materials, more and more evidence has proved the potential of BGs in soft tissue repair. BGs itself has antibacterial effect, which has obvious advantages in soft tissue repair. By changing its composition or combining with different materials such as functional ions, polymers and biological factors, BGs can promote wound healing more quickly and effectively. In this paper, the development of BGs was reviewed, and the possible mechanism of BGs accelerating wound repair was discussed by comparing with the mechanism of wound repair. Finally, the research trend of BGs in wound repair was interpreted.
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    Research progress of supercritical fluid extraction technology in wound healing 
    JIAN Yang, HE Yong, WEI Zairong.
    2022, 18 (4):  350. 
    Abstract ( 352 )   PDF (692KB) ( 400 )  
    Wound healing involves complicate pathophysiological process. With the mechanism of wound healing elucidated, wound healing has improved in a large extent as the result of new techniques and materials application. This paper focused on concluding the feature, principle, influence factors of supercritical fluid extraction (SFE) technique of CO2 and the research progress of this technique in wound healing, and provided the information of SFE in wound healing for further research of SFE in this field. 
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    Research and application progress of animal models for arteriovenous malformation
    DAI Mengting, CUI Jie.
    2022, 18 (4):  355. 
    Abstract ( 305 )   PDF (988KB) ( 271 )  
    Arteriovenous malformations (AVM) is a relatively rare and high risk vascular malformations. Although endovascular therapy, surgical therapy and combined therapy have been used in clinical practice, there are still many difficulties and challenges in the diagnosis and treatment of this disorder. In recent decades, a variety of experimental models have been developed to study the pathogenesis, hemodynamic changes and histology of AVM, so as to deepen the understanding of the disease and achieve better therapeutic effects. These models have their own advantages and disadvantages, but there is still a lack of animal models that can perfectly simulate various AVM in humans. In this paper, the recent research on AVM related animal models and their advantages and disadvantages in application were all reviewed.
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    Progress on the mouse model of cleft lip and palate 
    MA Yaping, WU Di.
    2022, 18 (4):  360. 
    Abstract ( 353 )   PDF (401KB) ( 195 )  
    Cleft lip and palate is a kind of congenital maxillofacial malformation, which has a high incidence in Asia. Many researches show that maxillofacial development of mouse share lots of similarities with human. Therefore, cleft lip and palate mouse model has always been an important tool to study human cleft lip and palate. The mouse model has advantages such as large numbers, long life, short growth cycle and easy to obtain, but also has many disadvantages such as limited types of models. Nowadays, there are various methods to establish cleft lip and palate mouse model. Using surgery to build cleft lip and palate mouse model is commonly used; Genetic engineering technology, such as transgenic technology, gene knockout technology, was used to establish cleft lip and palate mouse model. To establish cleft lip and palate mouse model, teratogenic agents such as dexamethasone and dioxins were applied to pregnant mice to influence the development of embryonic mice. In this paper, the methods of establishing mouse model of cleft lip and palate were reviewed.
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    Surgical treatment of traumatic earlobe cleft: A detailed description 
    LI Hanbo, ZHANG Qingguo.
    2022, 18 (4):  363. 
    Abstract ( 177 )   PDF (326KB) ( 139 )  
    Traumatic earlobe cleft is a common clinical earlobe injury, usually caused by external force pulling the earlobe. At present, there are many methods for the treatment of traumatic earlobe cleft, including straight-line technique, Z-plasty, L-plasty, flaps and non-surgical treatment techniques. In this paper, the concept, anatomy, type and classification, treatment principles and various treatment methods of traumatic ear lobe cleft were reviewed, in order to provide a clear overview and reference for clinicians.
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    Cause and treatment of olfactory dysfunction caused by facial contouring surgery
    SHU Kaiyi, ZHAO Jiuli, LIU Wei, ZHANG Zhiyong.
    2022, 18 (4):  366. 
    Abstract ( 136 )   PDF (365KB) ( 187 )  
     Olfactory function is one of the most important physiological functions. Facial contouring surgery is one of the most popular aesthetic surgeries. While olfactory dysfunction occasionally occurs after facial contouring surgery, which influences the living quality of cosmetic patients seriously. In this paper, the research reports on olfactory dysfunction after facial contouring surgery at home and abroad were reviewed, and its possible causes and treatments were discussed.
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    The impact of a plastic surgery elective course on medical undergraduates and a preliminary investigation of teaching mode 
    LIANG Hsin, KHOONG Yimin, HUANG Xin, LI Minxiong, LUO Shenying, LI Qingfeng, ZAN Tao
    2022, 18 (4):  369. 
    Abstract ( 165 )   PDF (821KB) ( 160 )  
    Objective To assess the necessity of introducing a plastic surgery course at the undergraduate level, which would enhance the current teaching method.  Methods The survey subjects were undergraduate students from Shanghai Jiao Tong University School of Medicine from the classes of 2017 to 2019, who were divided into three groups based on whether they had taken plastic surgery elective courses and/or clinical courses (internal medicine and surgical courses). Questionnaire was used to investigate the influence of the current elective course on students' comprehension level, interest, information acquisition channels, future career orientation in plastic surgery. Results A total of 111 questionnaires were distributed, with 103 of them being returned (98 of which were deemed valid). Following completion of the course, 92.31% of students expressed an increased interest in plastic surgery. The students are generally interested in common techniques of human body aesthetics and cosmetic surgery, and they desire clinical clerkships in addition to the current theoretical electives. Conclusion The introduction of a plastic surgery course at the undergraduate level is advantageous for enhancing students' understanding of plastic surgery and assisting them in choosing their future specialty. Furthermore, appropriate inclusion of the subject's frontier progress can broaden students' thinking and enrich the teaching mode, which is expected to improve overall teaching quality.
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    Impact of flipped classroom teaching model on clinical teaching effect of dermatovenereology
     CHEN Jun, XU Hui, SHEN Zhengyu, LI Meng, YANG Yali
    2022, 18 (4):  373. 
    Abstract ( 116 )   PDF (553KB) ( 170 )  
    Objective To investigate the impact of flipped classroom teaching model on clinical teaching effect of dermatovenereology. Methods A total of 40 eight-year students majoring in clinical medicine from Shanghai Jiao Tong University School of Medicine in the first semester of 2018-2019 academic year were selected and randomly divided into two groups. The observation group(n=20) adopted flipped classroom teaching mode and the control group(n=20) adopted traditional teaching mode. At the end of the course, the knowledge level (classroom performance, theoretical knowledge, clinical skills) of the two groups were compared. The cognition of medical education environment was evaluated by medical education environment measurement table. A questionnaire was designed to investigate students' satisfaction with teaching effect and study interest. Results Compared with the control group, the observation group had advantages in the level of knowledge, and the difference was statistically significant (P<0.05). The scores of learning, environment and social field and total score of teaching environment of students in the observation group was higher than those in the control group(P<0.05). The satisfaction of the students in the observation group was significantly higher than that in the control group(P<0.05). The learning interest  of the students in the observation group was significantly higher than those in the control group(P<0.05). Conclusion In the course of dermatovenereology teaching, the use of flipped classroom teaching method is more stimulate to improving students' interest in learning and cultivating their ability of self-learning, and the teaching effect is satisfactory.
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