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23 July 2020, Volume 11 Issue 6 Previous Issue    Next Issue

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Biocompatibility Research of Three-dimensional Printed Polylactic acid/Hydroxyapatite Composite with Bone Marrow Stromal Cells in vitro ZHANG Haifeng,DU Zijing,JIANG Wenbo,HAN Dong 2015, 11 (6):  349-353.  DOI: 10.3969/j.issn.1673-0364.2015.06.001 Abstract ( 371 )   Objective To explore the biocompatibility of three-dimensional printed polylactic acid-hydroxyapatite（3D printed PLA-HA） as the composite scaffolds with bone marrow stromal cells for selecting the suitable scaffold in bone tissue engineering. Methods GPF positive bone marrow stromal cells were seeded on three-dimensional printed polylactic acidhydroxyapatite composite scaffolds and beta-tricalcium phosphate（β-TCP） and then cultured in vitro. The cell adhesion and proliferation were observed by fluorescence microscope, and cell morphology was scrutinized by scanning electron microscopy. Cell Counting Kit 8（CCK8） were used to test the adhesion rate and proliferation of cells on two kinds of scaffolds at different time points; Alkaline phosphatase（ALP） activity were detected on the third, seventh and fourteenth day.Results Bone marrow stromal cells exhibited the adhesion and proliferation ability well in both three-dimensional printed polylactic acid-hydroxyapatite composite scaffolds and beta-tricalcium phosphate; The adhesion rate in β-TCP group was higher than in PLA-HA group（P〈0.05）, while the adhesion rate in PLA-HA group was still more than 60% after cultured for12 hours; The amount of cell proliferation（the value of OD） 4 and 7 days after culture in PLA-HA group were both higher than in β-TCP and control group respectively（P〈0.05） in vitro. The secretion of ALP 7 and 14 days after culture in groupPLA-HA as well as β-TCP were both higher than in control group 3（P〈0.05）. Meanwhile, the ALP secretion in PLA-HA group was higher on the third day compared with β-TCP group（P〈0.05）. Conclusion Three-dimensional printed polylactic acid-hydroxyapatite composite scaffolds present good biocompatibility in vitro and it can be selected as biomaterials in bone tissue engineering. Related Articles | Metrics

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Efficacy Evaluation of Sclerotherapy for Venous Malformations on Head and Neck Region Based on ROI-volumentry of MRI Assessment by Three-dimensional Reconstruction Measurement QIU Shanda,ZHANG Zimin,TAO Xiaofeng,YANG Xi,HU li,JIN Yunbo,CHEN Hui,LIN Xiaoxi 2015, 11 (6):  354-357.  DOI: 10.3969/j.issn.1673-0364.2015.06.002 Abstract ( 320 )   Objective To evaluate the efficacy of sclerotherapy for venous malformations（VMs） on head and neck region using ROI（region of interest） volumentry of MRI assessment by three-dimensional reconstruction measurement. Methods A retrospective analysis was selected, composed of 18 patients with VMs and fully qualified MRI data from October 2012 to March 2015. All patients were treated with sclerotherapy either once or twice and were divided into two groups. Percutaneous sclerotherapy was performed by direct injection of Ethanol（group A, n=11） and Polidocanol foam sclerosants（group B, n=7）.Patients underwent MRI before and after sclerotherapy. All the patients were followed up for 2 to 30 months after treatment.The MRI data was imported into Mimics18.0 software. The threshold ranges of VMs in ROI MRI were selected. The regression rates of lesions were compared before and after sclerotherapy. And the results were evaluated by the response evaluation criteria in VMs. Results According to the ROI-volumentry of MRI assessment by three dimensional reconstruction measurement and the response evaluation criteria in VMs, the effective rate in Group A was 91%, and 86% in Group B. Conclusion The method of ROI-volumentry of MRI assessment by three-dimensional reconstruction measurement can reflect and evaluate the regression rates of the lesions after sclerotherapy for patients with VMs on head and neck region.Our expertence demonstrate the high practicability and potential of this method. Related Articles | Metrics

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Effect of Periostin on the Survival Rate of Autologous Fat Transplantation LI Dong,HUANG Xiaoxiang,YUAN Jie,YU Zheyuan,XU Liang,XU Haisong,WEI Min 2015, 11 (6):  358-361.  DOI: 10.3969/j.issn.1673-0364.2015.06.003 Abstract ( 316 )   Objective To explore the effect of Periostin on the vascularization and survival rate of autologous fat transplantation, and to find out the role of Periostin in autologous fat transplantation. Methods The special effect of Periostin in inducing vascularization of adipose stem cells under hypoxia was observed in vitro. The expression of CD133 and CD144 of ASCs under hypoxia were tested by RT-PCR. Then, the adipose tissue compounded with Periostin was transplanted into nude mice, and the survival rate of the transplanted fat tissue was detected at different time points after transplantation.Results Periostin group showed a higher vascularization degree under hypoxia, compared with blank control group. The expression of CD133 and CD144 of ASCs under hypoxia in Periostin group were higher than in blank control group. Periostin composite with autologous fat transplantation improved the survival rate of fat transplantation at each time point（79.50% ±3.70%, 67.71% ±4.08%, 64.78% ±5.78%）, which were better than simple autologous fat transplantation（51.83% ±5.97%,39.45% ±10.32%, 34.20% ±10.61%）. Conclusion Periostin is able to improve fat survivability under hypoxia during the early stage, and promote the early stage vascularization of autologous fat granules after transplantation, which is beneficial to the survival of the transplanted fat tissue. Related Articles | Metrics

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Application of Autologous Fat Granules Injection for Nasolabial Groove Depression CHEN Qiang,MA Jiguang,Lv Changsheng,WANG Shujie 2015, 11 (6):  362-364.  DOI: 10.3969/j.issn.1673-0364.2015.06.004 Abstract ( 347 )   Objective To investigate the clinical application of autologous fat granules injection for nasolabial groove depression. Methods From November 2011 to December 2014, 91 cases with nasolabial groove depression were treated by autologous fat granules injection. The fat granule was harvested by liposuction from waist abdomen or lateral thigh under intravenous anesthesia and local infiltration anesthesia. After purified, the fat was injected into the nasolabial groove by multiple-layers, multiple-tunnels, multiple-points and low-amounts technique. Results After followed up for 6 to 36 months, 56 cases achieved the expected effect by injecting once and 35 cases achieved satisfactory results after reoperation.The amount of autologous fat for each side was 1.2 ml to 4.6 ml. No severe complication was observed. Conclusion Autologous fat granules injection for nasolabial groove depression is effective with few complication, and is worthy of clinical application. Related Articles | Metrics

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Clinical Application of Autologous Fat Grafting for Facial Contour Improvement and Facial Rejuvenation DENG Jingcheng,ZHANG Qi,CAO Weigang,LIU Lina,JIANG Zhaohua,LI Shengli 2015, 11 (6):  365-369.  DOI: 10.3969/j.issn.1673-0364.2015.06.005 Abstract ( 304 )   Objective To evaluate the clinical effects of autologous fat grafting for facial contour improvement and facial rejuvenation. Methods From January 2013 to December 2014, 140 cases aged 19 to 65 years with poor facial contour or aging face were treated by autologous fat grafting. After purification, washing and decanting, autologous fat harvested by liposuction from lower abdomen or thigh was injected into the recipient areas of face and neck, including fronto-temporal region, tear trough, malar and cheek areas, nose, nasolabial fold, chin, lip, earlobe and the other areas of face. Results All cases were followed up for 3 to 18 months, 130 cases gained satisfactory clinical results by injecting for once and the other10 cases acquired satisfactory clinical results with second injections. Except for 1 case of induration in the right temporal region and 5 cases of longer-lasting swelling over 2 months in naso-jugal creases, there were no obvious complications such as infection, fat necrosis and fibrosis. The satisfactory rate was 93.6% in total. Conclusion Autologous fat transfer is a simple, safe and quite effective method for facial contour improvement and facial rejuvenation, which can remodel facial appearance, recover facial volume and promote facial rejuvenation naturally. Most of the patients were satisfied with the soft,natural appearance of the face. Related Articles | Metrics

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Super Long Pedicle Island Flap for Repairing Large Tissue Defect of the Heel JI Yi,CHEN Zhuo,CHEN Liang,LIU Jianyi,WANG Zhenxiang 2015, 11 (6):  370-372.  DOI: 10.3969/j.issn.1673-0364.2015.06.006 Abstract ( 386 )   Objective To explore the clinical effects of super long vascular pedicle island flap for repairing large tissue defect of heel. Methods From January 2012 to February 2014, 12 cases with large soft tissue defect of heels were treated with super long pedicle island flap. The nerve nutrient vessels of super long island flaps were designed on the calf and foot dorsal to repair the huge heel wound after rotation. And the donor wound was closed by skin graft. Results Wear-resisting transplant flap with good appearance were achieved, and no bloating and pigmentation were observed. All the patients were followed up for 6-12 months, the donor areas had no obvious secondary deformities and no complication was observed.Conclusion Once performed properly, super long vascular pedicle of island flap can provide reliable blood supply and very long graft, it is an ideal method for primary or secondary wound repair of heel. Related Articles | Metrics

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Clinical Application of the Expanded Neck Flap Prefabricated by Superficial Temporal Fascia for Facial Resurfacing BAI Guofang,XIE Feng 2015, 11 (6):  373-378.  DOI: 10.3969/j.issn.1673-0364.2015.06.007 Abstract ( 435 )   Objective To explore the clinical effect of the expanded neck flap prefabricated by superficial temporal fascia for facial resurfacing. Methods From June 2013 to March 2015, 10 patients with large facial deformity were treated with the expanded neck flap prefabricated by superficial temporal fascia. The superficial temporal fascia was separated, rotated reversely, and located beneath subcutaneous tissue of the planned expanded neck area over a tissue expander. After expansion for 5-6 months to achieve enough volume, the expected resurfacing shape was designed and raised, and an island flap based on superficial temporal fascia was harvested and rotated to resurface the facial defect. Results The expansion volume reached 400-800 ml after prefabrication. The flap size ranged from 8 cm × 9.5 cm to 14 cm × 14 cm, with an average area of 129 cm2. Ten patients were treated by this method, and there were no major complications in the expansion process, such as ischemia and necrosis. All the patients were followed up for 6 months to 1 year, there was an excellent color and texture match with surrounded facial skin. Conclusion The blood supply could increase effectively after being prefabricated with superficial temporal fascia, and more expanded skin could be harvested to repair large facial defect. Related Articles | Metrics

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Inferior Trapezius Myocutaneous Flap for Repairing High-Voltage Electrical Injury in Head and Neck Region XU Jin,YU Yong,WU Zhuming 2015, 11 (6):  376-378.  DOI: 10.3969/j.issn.1673-0364.2015.06.008 Abstract ( 394 )   Objective To explore the application of inferior trapezius myocutaneous flap for repairing high-voltage electrical injury in head and neck region. Methods Since 2007, 5 patients with high-voltage electrical injury in head and neck region were treated with inferior trapezius myocutaneous flap. Results All the inferior trapezius myocutaneous flaps were survived. The patients were followed up for 3-24 months, satisfactory appearance and functional recovery were achieved and no shoulder dysfunction was observed. Conclusion The inferior trapezius myocutaneous flap has the advantage of rich blood supply, simple operative procedure, powerful anti-infection ability and little effect on shoulder function, and is suitable for the repair of high-voltage electrical injury in head and neck region. Related Articles | Metrics

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Combined Treatment of Oblate Anterior Naris in Rhinoplasty SUN Xiaoyin,DUN Huichuan,YUAN Jie,WEI MIN 2015, 11 (6):  379-408.  DOI: 10.3969/j.issn.1673-0364.2015.06.009 Abstract ( 346 )   Objective To investigate the methodology of improving the morphology of oblate anterior naris. Methods From December 2014 to May 2015, 57 cases with congenital inclinate oval-shaped nostrils were measured by digital photographies before and after operation. The morphology of anterior naris was corrected by open approach rhinoplasty with interdomal suture, columellar-upper lip V-Y sliding flap and autologous cartilage/prosthesis implantation. Results The surgical incisions healed primarily in all patients. The nostril direction and angle was obviously improved. All the patients were followed up for 6 to 12 months, invisible scar was observed, no recurrence and undesirable complications were observed. Conclusion The interdomal suture and columellar-upper lip V-Y sliding flap can effectively correct the nostril orientation and the nasal base shape. Related Articles | Metrics

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Advances of Seed Cells for Constructing Biological Pacemaker JI Ruijuan,YANG Xiangqun,Research Center of Regenerative Medicine 2015, 11 (6):  381-385.  DOI: 10.3969/j.issn.1673-0364.2015.06.010 Abstract ( 314 )   With the rapid development of stem cells, tissue engineering and regenerative medicine, cell transplantation and tissue engineering to construct biological pacemaker are expected to become the future direction. It is very crucial to find the ideal seed cells. Nowadays, the seed cells used for constructing the biological pacemaker includes： adult cells and stem cells. In this paper, the advances of seed cells for constructing biological pacemaker were reviewed. Related Articles | Metrics

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Testing Methods for the Biological Performance of Antibacterial Materials XU Qiming,ZHOU Shengbo,MAO Hailei,WANG Bin 2015, 11 (6):  386-395.  DOI: 10.3969/j.issn.1673-0364.2015.06.011 Abstract ( 486 )   Antibacterial materials can be divided into three categories, inorganic, natural organic and synthetic organic.A series of biological performance testing are required for the new antibacterial materials, including antibacterial activity,biocompatibility and material properties. In this paper, the testing methods for their biological performance were reviewed. Related Articles | Metrics

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Etiology, Classification and Treatment of Brachydactyly YANG Xi,WANG Bin 2015, 11 (6):  389-395.  DOI: 10.3969/j.issn.1673-0364.2015.06.012 Abstract ( 750 )   Brachydactyly（BD） is a congenital deformity that refers to shortening of the hands/feet due to small or missing metacarpals/metatarsals and/or phalanges characterized by bone dysostosis. According to Bell＇s classification, BD can be divided into to five categories： A to E. Recent years, new subtypes were advocated. Among these, BDA3 and BDD may be the most common subtypes. The deformity was close related to familial inheritance and gene mutations. In this review, the advancements in etiology, classification and treatments of brachydactyly were summarized. Related Articles | Metrics

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Application and Research Progress on Transverse Myocutaneous Gracilis Flap in Breast Reconstruction XUE Bingjian,LIU Liqiang 2015, 11 (6):  396-399.  DOI: 10.3969/j.issn.1673-0364.2015.06.013 Abstract ( 378 )   Autologous tissue reconstruction is regarded as the standard for breast reconstruction. Since it was first proposed in 1992, the transverse myocutaneous gracilis flap（TMG flap）, as an alternative, has been developed a lot in autologous breast reconstruction for its distinct advantages and low donor-site morbidity. The anatomy, design, clinical application and research progress were mainly reviewed in this paper. Related Articles | Metrics

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The Influence of Biotic Factors and Medicines on the Survival Rate of Autologous Fat Grafting LI Pengfei,YU Li 2015, 11 (6):  400-403.  DOI: 10.3969/j.issn.1673-0364.2015.06.014 Abstract ( 348 )   Autologous fat grafting has been frequently used in clinic recently. Autologous fat can perfectly fit in the host area. What＇s more, it has characters like convenient access, abundant in amount, well-shaped, low-cost, as well as few complications and low risks. However, uncertain survival rate is a big problem for autologous fat grafting. In this paper the influence of biotic factors and medicines on autologous fat grafting were reviewed. Related Articles | Metrics

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Estrogen Effect and the Mechanism on Skin Aging MENG Fei,XI Linhe,ZHANG Jie,WANG Yan,WANG Li 2015, 11 (6):  404-408.  DOI: 10.3969/j.issn.1673-0364.2015.06.015 Abstract ( 476 )   Skin is the largest target organ for estrogen among reproductive organs. During postmenopausal period, the decline in estrogen level accelerates the aging of skin. Estrogen replacement therapy（HRT） or local application of estrogen in the treatment of female menopause symptoms can improve the skin condition, which increases the amount of collagen and elastic fibers in the dermis and extra cellular matrix, thus improves the thickness, elasticity and moisturizing ability of skin and reduces wrinkles, but its mechanism remains to be further researched. In this paper, the effect of estrogen on the skin and the mechanism were summarized. Related Articles | Metrics